ANP32B Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA035034A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: HT-29 whole cell lysate(30µg), THP-1 whole cell lysate(30µg), U-251MG whole cell lysate(30µg), A549 whole cell lysate(30µg), K562 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), A431 whole cell lysate(30µg), HeLa whole cell lysate(30µg) All lanes: ANP32B antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time：30s IHC image of CSB-RA035034A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA035034A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing jurkat cells stained with CSB-RA035034A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

Q92688

Target Names

ANP32B

Alternative Names

Acidic leucine-rich nuclear phosphoprotein 32 family member B (Acidic protein rich in leucines) (Putative HLA-DR-associated protein I-2) (PHAPI2) (Silver-stainable protein SSP29), ANP32B, APRIL PHAPI2

Species Reactivity

Human

Immunogen

A synthesized peptide from human ANP32B protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8C9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

ANP32B, also known as acidic leucine-rich nuclear phosphoprotein 32 family member B, plays important roles in chromatin remodeling, cell proliferation, and apoptotic regulation. This multifunctional protein has garnered significant research interest due to its involvement in transcriptional regulation and its emerging connections to cancer biology, making it a valuable target for investigators studying cellular homeostasis and disease mechanisms.

This recombinant monoclonal antibody, clone 8C9, offers the consistency and reliability that demanding experimental workflows require. Produced using recombinant technology with a defined sequence, this antibody eliminates the lot-to-lot variability that can compromise longitudinal studies and reproducibility. The rabbit host-derived IgG format provides excellent signal-to-noise characteristics while maintaining compatibility with standard secondary detection systems.

Validation data demonstrates robust performance across multiple applications. Western blot analysis confirms specific detection of ANP32B at the expected 29 kDa molecular weight across an extensive panel of human cell lines, including HT-29, THP-1, U-251MG, A549, K562, MCF-7, Jurkat, A431, and HeLa cells, providing confidence when working with diverse cellular models. Immunohistochemistry validation in paraffin-embedded human lung cancer and testis tissues shows reliable tissue staining with citrate buffer antigen retrieval, while flow cytometry analysis in Jurkat cells demonstrates clear population separation from isotype controls, confirming utility for single-cell analysis applications.

The antibody is supplied in a glycerol-containing buffer optimized for long-term storage stability. Whether investigating ANP32B's role in chromatin dynamics, exploring its function in proliferative signaling, or examining its expression patterns in tumor biology, this antibody provides the technical foundation for meaningful experimental insights.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Multifunctional protein that is involved in the regulation of many processes including cell proliferation, apoptosis, cell cycle progression or transcription. Regulates the proliferation of neuronal stem cells, differentiation of leukemic cells and progression from G1 to S phase of the cell cycle. As negative regulator of caspase-3-dependent apoptosis, may act as an antagonist of ANP32A in regulating tissue homeostasis. Exhibits histone chaperone properties, able to recruit histones to certain promoters, thus regulating the transcription of specific genes. Plays also an essential role in the nucleocytoplasmic transport of specific mRNAs via the uncommon nuclear mRNA export receptor XPO1/CRM1. Participates in the regulation of adequate adaptive immune responses by acting on mRNA expression and cell proliferation.; (Microbial infection) Plays an essential role in influenza A and B viral genome replication. Plays also a role in foamy virus mRNA export from the nucleus to the cytoplasm.

Gene References into Functions

According to the cellular function of the characterized targets of ProTalpha, and the evolution in the composition of the diverse ProTalpha-complexes when proliferation activity was reduced or apoptosis induced, leads to hypothesized that ProTalpha interactions might be related to the proliferation activity and control of the cell survival. PMID: 29106904
ANP32B modulates Bad phosphorylation as well as Bak and Bax expression, resulting in regulation of apoptosis in HCC. These findings indicate the potential value of ANP32B as a therapeutic target for HCC PMID: 28486557
Caspase-3-resistant uncleavable form of acidic leucine-rich nuclear phosphoprotein 32B potentiates leukemic cell apoptosis. PMID: 25483709
ANP32B is a nuclear target of henipavirus M proteins. PMID: 24823948
this is the first demonstration that ANP32B expression was down-regulated during differentiation induction of leukemic cells by all-trans retinoic acid. PMID: 22705300
the interaction of ANP32B with the core histones H3-H4 occurs on its concave side, and both the acidic and hydrophobic residues that compose the concave surface are critical for histone binding. PMID: 20538007
ANP32B is a direct substrate of caspase-3. It is cleaved at the sequence of Ala-Glu-Val-Asp, after Asp-163. The reduced expression of endogenous ANP32B by specific siRNA enhances caspase-3 activation & apoptosis induction by NSC606985 & etoposide. PMID: 20015864
regulatory roles of oncoprotein ProT and tumor suppressor PHAP in apoptosis PMID: 12522243
recruitment of ANP32B onto the promoter region requires KLF5 and results in promoter region-specific histone incorporation and inhibition of histone acetylation by ANP32B PMID: 18039846
The CK2 alpha' phosphorylates APRIL and therefore is responsible for the regulation of the nucleocytoplasmic translocation of CD83 mRNA. PMID: 19130553

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Subcellular Location

[Isoform 1]: Nucleus. Cytoplasm.; [Isoform 2]: Cytoplasm. Note=Lacks a nuclear localization signal.

Protein Families

ANP32 family

Tissue Specificity

Expressed in heart, lung, pancreas, prostate and in spleen, thymus and placenta.

Database Links

HGNC: 16677

KEGG: hsa:10541

STRING: 9606.ENSP00000345848

UniGene: Hs.730654

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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