Product Details

Uniprot No.

Target Names

APBB1

Alternative Names

Adaptor protein FE65a2 antibody; Amyloid beta (A4) precursor protein binding family B member 1 antibody; Amyloid beta A4 precursor protein binding family B antibody; Amyloid beta A4 precursor protein binding family B member 1 antibody; Amyloid beta A4 precursor protein-binding family B member 1 antibody; Amyloid beta precursor protein binding family B member 1 antibody; APBB 1 antibody; APBB1 antibody; APBB1_HUMAN antibody; FE 65 antibody; Fe65 protein antibody; Protein Fe65 antibody; RIR antibody; stat like protein antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human APBB1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

25D4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Transcription coregulator that can have both coactivator and corepressor functions. Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its transcription activation activity. Functions in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s). Involved in hippocampal neurite branching and neuromuscular junction formation, as a result plays a role in spatial memory functioning. Plays a role in the maintenance of lens transparency. May play a role in muscle cell strength.

Gene References into Functions

Fe65 is an adaptor protein involved in both processing and signaling of the Alzheimer-associated amyloid-beta precursor protein, APP. Here, the subcellular localization was further investigated using TAP-tagged Fe65 constructs expressed in human neuroblastoma cells. Our results indicate that PTB2 rather than the WW domain is important for the nuclear localization of Fe65. PMID: 28323844
Fe65 Ser289 phosphorylation did not affect the transcriptional activity of the Fe65-APP complex, in contrast to the previously described Ser228 site. PMID: 27176072
Our findings imply that APBB1 plays an important role in the maintenance of EMT-associated CSC-like properties and gamma-radiation resistance via activation of IGF1Rbeta/AKT/GSK3beta pathway in lung cancer cells, highlighting APBB1 as a potential target for therapeutic cancer treatment. PMID: 27836546
Targeted enhancement of the signaling through the Fe65-cortactin pathway, by either HDAC6 inhibition or Tip60 activation, may lead to the development of new therapeutic drugs that are effective for patients with metastatic breast cancers. PMID: 26166158
FE65 influences APP degradation via the proteasome, and phosphorylation of FE65 Ser(610) by SGK1 regulates binding of FE65 to APP, APP turnover and processing. PMID: 26188042
A novel phosphorylation site was identified within Fe65 which mediates gene transcription. PMID: 25397632
The SV2A/FE65 interaction might play a role in synaptic signal transduction. PMID: 24284412
Data indicate that Fe65 is a positive estrogen receptor alpha (ERalpha) transcriptional regulator. PMID: 24619425
FE65 interactions with BLM and MCM proteins may contribute to the neuronal cell cycle re-entry observed in brains affected by Alzheimer's disease. PMID: 23572515
A ternary complex consisting of AICD, FE65, and TIP60 down-regulates Stathmin1. PMID: 22902274
Both amyloid-beta precursor protein and Fe65 are co-localized in model Hirano bodies associated with Alzheimer's disease. PMID: 20133016
Fe65 carries out different functions depending on its location in the regulation of Notch1 signaling. PMID: 22199353
A novel FE65 isoform and the regulation of the splicing events leading to its production may contribute to elucidating neuronal specific roles of FE65 and its contribution to Alzheimer's disease pathology. PMID: 21824145
Phosphorylation of LRP1 regulates the interaction with Fe65. PMID: 21968187
Fe65 binds preferentially to low-density lipoprotein receptor-related protein (LRP) carboxyl terminus when phosphorylated at tyrosine-4507 and in complex with amyloid precursor protein (APP). PMID: 21650223
Fe65 and Dab1 compete for binding to APP. Dab1 significantly decreased the amount of APP bound to LRP and the level of secreted APP and APP-CTF in LRP expressing cells PMID: 20568118
reduced levels of Sp1 resulted in downregulation of endogenous FE65 mRNA and protein PMID: 20091743
The data of this demonstrated that the induction of AICD/Fe65 or transgelin significantly alters actin dynamics and mitochondrial function in neuronal cells PMID: 20405578
The transcriptional activity of the APP intracellular domain-Fe65 complex is inhibited by activation of the NF-kappaB pathway. PMID: 12653567
Adjusting for age and sex, we found a slight risk associated with the deletion in intron 13 of the APBB1 gene for subjects less than 65 years. PMID: 12727304
gamma-Secretase cleavage and binding to FE65 regulate the nuclear translocation of the intracellular C-terminal domain (ICD) of the APP family of proteins. PMID: 12779321
APP and Fe65 mediate transactivation with low density lipoprotein receptor-related protein PMID: 12888553
Abnormal accumulations of the amyloid-beta precursor protein associated with the aging cellular muscle fibers and appear to be the key pathogenic event in iclusion-body myositis. PMID: 14569203
Alcadein and amyloid beta-protein precursor regulates FE65-dependent gene transactivation PMID: 15037614
Fe65 is activated by the APP intracellular domain during transcriptional transactivation PMID: 15044485
p65FE65 may be an intracellular mediator in a signaling cascade regulating alpha-secretion of APP PMID: 15647266
The present work provides evidence that FE65 plays a role in the regulation of amyloid precursor protein processing in an in vivo transgenic mouse model. PMID: 15816856
multiple interactions of AICD with FE65 and 14-3-3gamma modulate FE65-dependent gene transactivation PMID: 16223726
FE65 is the key agent of Gal4DB-mediated transcriptional transactivation, whereas Tip60 is an FE65-associated repressor. PMID: 16332686
Notch1 intracellular domain plays the role of a negative regulator in AICD signaling via the disruption of the AICD-Fe65-Tip60 trimeric complex. PMID: 17368826
Nek6 binds to Fe65 through its (267)PPLP(270) motif; the protein-protein interaction between Nek6 and Fe65 regulates their subcellular localization and cell apoptosis PMID: 17512906
We demonstrated that treatment with EGCG reduced the A beta levels by enhancing endogenous APP nonamyloidogenic proteolytic processing. PMID: 17590240
Results describe the crystal structures of the human FE65 WW domain (residues 253-289) in the apo form and bound to the peptides PPPPPPLPP and PPPPPPPPPL, which correspond to human Mena residues 313-321 and 347-356, respectively. PMID: 17686488
Fe65 regulation of APP proteolysis may be integrally associated with its nuclear signaling function, as all antecedent proteolytic steps prior to release of Fe65 from the membrane are fostered by the APP-Fe65 interaction PMID: 17855370
APP-regulated FE65 plays an important role in the early stress response of cells and that FE65 deregulated from APP induces apoptosis. PMID: 18468999
Thyroid cancers are characterized by APP upregulation, increased membrane targeting of the APP ectodomain and significantly increased mRNA levels of the APP scaffold proteins JIP1, ShcA and Fe65. PMID: 18480379
crystallographic analysis of the human Fe65-phosphotyrosine domain PMID: 18550529
Single nucleotide polymorphisms in APBB1 gene is associated with nicotine dependence. PMID: 18777128
the beta-sheet edge in some natively folded amyloid oligomers is designed positively to prevent beta aggregation PMID: 18800165
Study determined the crystal structure of the carboxy-terminal APP intracellular domain in complex with the C-terminal phosphotyrosine-binding (PTB) domain of Fe65; The unique interface involves the NPxY PTB-binding motif and two alpha helices. PMID: 18833287
Dexras1 functions as a suppressor of FE65-APP-mediated transcription, and FE65 tyrosine 547 phosphorylation enhances FE65-APP-mediated transcription, at least in part, by modulating the interaction between FE65 and Dexras1 PMID: 18922798
The protein-protein interaction between the WW domain of Fe65 and the putative binding motif of Nedd4-2 down-regulates Fe65 protein stability and subcellular localization through its ubiquitylation, to contribute to cell apoptosis. PMID: 19381069

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Subcellular Location

Cell membrane. Cytoplasm. Nucleus. Cell projection, growth cone. Nucleus speckle.

Tissue Specificity

Highly expressed in brain; strongly reduced in post-mortem elderly subjects with Alzheimer disease.

Database Links

HGNC: 581

OMIM: 602709

KEGG: hsa:322

STRING: 9606.ENSP00000299402

UniGene: Hs.372840

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Code	CSB-RA981854A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA981854A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA981854A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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