Product Details

Uniprot No.

P25705

Target Names

ATP5A1

Alternative Names

ATP synthase subunit alpha, mitochondrial, ATP5A1, ATP5A ATP5AL2 ATPM

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human ATP5A1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

5G11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

ATP5A1, also known as ATP synthase subunit alpha, serves as a critical component of the mitochondrial F1F0-ATP synthase complex responsible for the majority of cellular ATP production. This enzyme complex plays a fundamental role in oxidative phosphorylation, making ATP5A1 an essential marker for studying mitochondrial function, cellular metabolism, and bioenergetics. Dysregulation of ATP synthase activity has been implicated in various pathological conditions, including cancer metabolism alterations and neurodegenerative diseases, positioning this target at the intersection of multiple active research fields.

This recombinant monoclonal antibody, generated from clone 5G11, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined sequences rather than traditional hybridoma methods, researchers benefit from lot-to-lot uniformity that supports longitudinal studies and ensures comparable results across experiments.

Validation studies demonstrate robust performance across multiple applications. In western blot analyses at 1:2000 dilution, this antibody detects a clean 60 kDa band consistent with the predicted molecular weight of ATP5A1. Detection has been confirmed in diverse human cell lines including HepG2, MCF-7, 293, and HL60, providing flexibility for researchers working with different cellular models. Cross-species reactivity extends to mouse tissues, with successful detection demonstrated in both heart and brain lysates. For immunohistochemistry applications, validation in paraffin-embedded human liver and kidney tissues at 1:100 dilution shows reliable staining using standard citrate buffer antigen retrieval protocols.

Whether investigating mitochondrial dysfunction in cancer models, exploring metabolic signaling pathways, or characterizing tissue-specific expression patterns, this antibody provides a dependable tool for ATP5A1 detection across your experimental platforms.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites. Binds the bacterial siderophore enterobactin and can promote mitochondrial accumulation of enterobactin-derived iron ions.

Gene References into Functions

Using systems biology techniques to study gene coexpression network, ATP5A1 was identified and found highly expressed in normal kidney than clear cell renal cell carcinoma (ccRCC) tissues of each grade. Functional and pathway enrichment analysis demonstrated that ATP5A1 is overrepresented in pathway of oxidative phosphorylation, which associated with tumorigenesis and tumor progression. PMID: 29207195
This cohort study showed that the ATP5A1 gene was down regulation between patients with idiopathic Parkinson disease and controls PMID: 28916538
these data highlight a key role of the P2Y1/PI3Kbeta axis in endothelial cell proliferation downstream of ecto-F1-ATPase activation by apoA-I. Pharmacological targeting of this pathway could represent a promising approach to enhance vascular endothelial protection. PMID: 28578353
These findings, together with the previously reported inhibition of respiratory complex I, show that depression of the activity of oxidative phosphorylation enzymes is involved in the cell growth inhibitory action of ATRA. PMID: 27856255
High mRNA levels of ATP5A1 are associated with glioblastoma. PMID: 26526033
Hemoglobin - a novel ligand of hepatocyte ectopic F1-ATPase PMID: 26769832
Here, we found that ATP synthase subunit alpha (ATP5A) was O-GlcNAcylated at Thr432 and ATP5A O-GlcNAcylation was decreased in the brains of AD patients and transgenic mouse model PMID: 26358770
Mitochondrial calpain-1 disrupts ATP synthase, leading to mitochondrial reactive oxygen species generation, which promotes proinflammatory response and myocardial dysfunction during endotoxemia. PMID: 26246018
Studies indicate that the F-ATP synthase can reversibly undergo a Ca(2+)-dependent transition to form a channel that mediates the permeability transition. PMID: 25999424
A mutation in ATP5A1 causes a fatal neonatal mitochondrial encephalopathy. PMID: 23599390
Studies indicate that F1-ATPase (F1) is a rotary motor protein driven by ATP hydrolysis and the minimum complex of F1 for function as a rotary motor is the alpha3beta3gamma subcomplex. PMID: 23395605
Homozygous mutations in this gene are the likely cause of mitochondrial disease in two sisters, with support from a yeast model. PMID: 23596069
F1-ATPase at the cell surface of colonic epithelial cells has a role in mediating cell proliferation PMID: 23055519
An interactive proteomics study was done to examine proteins that bind heterocomplexes with ABCC1 using coimmunoprecipitation and MS/MS analyses. We found that ATP synthase alpha binds to ABCC1 in plasma membranes with a ratio of 2:1. PMID: 22188235
Antibodies to the beta- and gamma-subunits of F(1)-ATPase are further antimitochondrial antibodies in primary biliary cirrhosis. PMID: 22098431
A selective Cys in ATP synthase alpha subunit is targeted by multiple oxidative posttranslational modifications suggesting that this Cys residue may act as a redox sensor modulating ATP synthase function. PMID: 21817160
Data suggest that F1-ATPase catalytic site show the correlation between the phosphate binding and the tightening of the alphabeta-interface. PMID: 21481781
This protein has been found differentially expressed in the anterior cingulate cortex in men patients with schizophrenia. PMID: 20381070
The alpha-chain of ATP synthase is implicated in neurofibrillary degeneration of Alzheimer's disease that is illustrated by the cytosolic accumulation of this mitochondrial protein, which belongs to the mitochondrial respiratory system. PMID: 12614671
Within the structurally-confined internal aqueous cavity of the F1-motor of ATP synthase, function results from free energy changes that shift the balance between interfacial charge hydration and interfacial hydrophobic hydration. PMID: 16378738
analysis of vascular endothelial ectoadenylate kinase and plasma membrane ATP synthase PMID: 16714292
Higher levels of ATP5a1 expression are associated with certain Single Nucleotide Polymorphisms and with TP53 mutation. PMID: 19261598
These data show that an linoleic acid-phospholipid induced stimulation in hepatic HDL secretion is related to the expression and function of membrane ATP metabolizing proteins. PMID: 19717637
This protein has been found differentially expressed in the Wernicke's Area from patients with schizophrenia. PMID: 19405953
This protein has been found differentially expressed in the dorsolateral prefrontal cortex from patients with schizophrenia. PMID: 19110265

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Involvement in disease

Combined oxidative phosphorylation deficiency 22 (COXPD22); Mitochondrial complex V deficiency, nuclear 4 (MC5DN4)

Subcellular Location

Mitochondrion. Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. Cell membrane; Peripheral membrane protein; Extracellular side.

Protein Families

ATPase alpha/beta chains family

Tissue Specificity

Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord.

Database Links

HGNC: 823

OMIM: 164360

KEGG: hsa:498

STRING: 9606.ENSP00000282050

UniGene: Hs.298280

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Code	CSB-RA159926A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: HepG2 whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HL60 whole cell lysate, Mouse Heart tissue, Mouse Brain tissue All lanes: ATP5F1A antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 55, 58 kDa Observed band size: 60 kDa IHC image of CSB-RA159926A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA159926A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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ATP5A1 Recombinant Monoclonal Antibody

Product Details

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Target Background