Product Details

Uniprot No.

O43683

Target Names

BUB1

Alternative Names

Mitotic checkpoint serine/threonine-protein kinase BUB1 (hBUB1) (EC 2.7.11.1) (BUB1A), BUB1, BUB1L

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human Bub1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9C11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

BUB1 serves as a critical mitotic checkpoint kinase that ensures accurate chromosome segregation during cell division. By phosphorylating key substrates at the kinetochore, BUB1 helps maintain the spindle assembly checkpoint, preventing premature anaphase onset until all chromosomes achieve proper bipolar attachment. Dysregulation of this checkpoint mechanism has been implicated in chromosomal instability and aneuploidy, making BUB1 a significant target for researchers investigating cancer biology and fundamental cell cycle control.

This recombinant monoclonal antibody, generated from clone 9C11, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined sequences rather than traditional hybridoma methods, you can expect reliable performance across experiments and between lots, eliminating a common source of variability in long-term studies.

Validated for Western blot and ELISA applications, this antibody has demonstrated robust detection of human BUB1 across multiple cell lines. Western blot analysis confirms specific detection in HeLa, K562, and HepG2 whole cell lysates at dilutions ranging from 1:500 to 1:5000, providing flexibility to optimize signal intensity for your specific experimental conditions. The observed band at approximately 130 kDa runs slightly higher than the predicted molecular weights of 116–123 kDa, which likely reflects post-translational modifications such as phosphorylation events that are characteristic of this active kinase during mitotic regulation.

Whether you are investigating spindle checkpoint mechanisms, characterizing mitotic defects in cancer models, or exploring BUB1's broader roles in genome stability, this antibody provides a dependable tool for advancing your cell biology research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Required for centromeric enrichment of AUKRB in prometaphase. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.

Gene References into Functions

Conserved domain 1 (CD1) in human Bub1 binds directly to Mad1 and a phosphorylation site exists in CD1 that stimulates Mad1 binding and SAC signalling. PMID: 28604727
Finally, the authors show that the kinetochore localization of BUB1 and BUBR1 decreases with the age of the oocyte donors. This could contribute to oocyte aneuploidy. PMID: 28287092
Small sequence differences in Bub1 and BubR1 direct Bub3 to different phosphorylated targets in the spindle assembly checkpoint signaling cascade. PMID: 28943088
MiR-490-5p could regulate TGFbeta/Smad signaling pathways by inhibiting BUB1. PMID: 28810242
Therefore, Mps1 promotes checkpoint activation through sequentially phosphorylating Knl1, Bub1, and Mad1. This sequential multi-target phosphorylation cascade makes the checkpoint highly responsive to Mps1 and to kinetochore-microtubule attachment. PMID: 28072388
Chromosomal rearrangement resulting in BUB1 haploinsufficiency identified in a patient with polycystic liver disease. BUB1 may imply germline causes of genetic instability in PLD. PMID: 27552964
Results report that BubR1 and RepoMan bind directly to PP2A-B56 using an LSPIxE short linear motif (SLiM), where phosphorylation of the Ser residue enhances binding. RepoMan and BubR1 bind B56 using both hydrophobic and electrostatic interactions. PMID: 27998540
Inhibition of Bub1 kinase impaired chromosome arm resolution but exerted only minor effects on mitotic progression or spindle assembly checkpoint function. PMID: 26885717
Study shows that BUB1beta and PDZ binding kinase are up-regulated in breast cancer tumors and their expression is associated with improved overall survival (OS) in basal-like tumors but worse OS in luminal and untreated patients. PMID: 26897635
Bub1 may be associated with cancer stem cell potential. PMID: 26522589
Both Bub1 and BubR1 bind stably to Bub3 that is required for kinetochore localization of the proteins PMID: 26260062
Bub1-Plk1-mediated phosphorylation of Cdc20 constitutes an anaphase-promoting complex or cyclosome-inhibitory mechanism that is parallel, but not redundant, to mitotic checkpoint complex formation. PMID: 26912231
Data show that mitotic checkpoint kinase Bub1 is an active kinase regulated by intra-molecular phosphorylation at the P+1 loop. PMID: 26658523
human BUB1 does not associate stably with the MAD2 activator MAD1 (also known as MAD1L1) and, although required for accelerating the loading of MAD1 onto kinetochores, BUB1 is dispensable for the maintenance of steady-state levels of MAD1 there PMID: 26148513
MAD2L1 and BUB1 may play important roles in breast cancer progression, and measuring the expression of these genes may assist the prediction of breast cancer prognosis. PMID: 26287798
Data provide novel insight into the regulation and kinetochore residency of Bub1 and indicate that its localization is dynamic and tightly controlled through feedback autophosphorylation. PMID: 26399325
Bub1 coordinates checkpoint signalling by distinct domains for RZZ and BubR1 recruitment and localizes antagonistic checkpoint activities. PMID: 26031201
BUB1B expression was highly correlated to CDC20 and CCNB1 expression in multiple myeloma cells, leading to increased cell proliferation.Bub1 expression promotes cell proliferation and leads to poor survival in multiple myeloma patients PMID: 25698537
BubR1 is a component of the Mitotic checkpoint complex and was reported to be overexpressed in Breast cancer. BubR1 overexpression was correlated with poor survival in early stage Breast cancer patients. PMID: 25731686
Bub1 in complex with LANA recruits PCNA to regulate Kaposi's sarcoma-associated herpesvirus latent replication and DNA translesion synthesis. PMID: 26223641
Study provides structural and functional insights into the regulation of the key mitotic kinase Bub1 and highlights a mode of substrate-specific kinase activation through intramolecular autophosphorylation of the substrate binding loop. PMID: 25308863
The kinase activity of the Ser/Thr kinase BUB1 promotes TGF-beta signaling. PMID: 25564677
Data show sequential multisite regulation of the microtubule-associated protein KNL1-kinase-adaptor complex BUB1/BUB3 interaction. PMID: 25661489
Bub1, but not BubR1, enhances binding of Bub3 to phosphorylated kinetochores. PMID: 25611342
ABBA motifs in BUBR1 and BUB1 are necessary for the spindle assembly checkpoint to work at full strength and to recruit CDC20 to kinetochore PMID: 25669885
The bioinformatics analysis suggested that this variant may regulate the transcriptional ability of BUB1 PMID: 24711138
BUB1 is co-expressed with AURKA and AURKB suggesting biological relationship between these spindle cell components in primary and metastatic advanced-stage ovarian serous carcinoma PMID: 24756216
BUB1 and BUBR1 inhibition decreases proliferation and shows radiosensitizing effects on pediatric glioblastoma cells PMID: 23728478
Bub1-mediated H2A phosphorylation penetrates kinetochores and that this histone mark contributes to a tension-sensitive Sgo1-based molecular switch for chromosome segregation. PMID: 24055156
Studied the significance of budding uninhibited by benzimidazoles-1 (Bub1) and mitotic arrest deficient-2 (Mad2) expression in endometrial carcinoma. PMID: 24654460
Mutations in BUB1 and BUB3 cause mosaic variegated aneuploidy and increase the risk of colorectal cancer at a young age. PMID: 23747338
These results all indicated that BUB1 played important roles in the proliferation and/or progression of the breast carcinoma, and nuclear BUB1 immunohistochemical status is also considered a potent prognostic factor in human breast cancer patients. PMID: 23288590
IRAK1, CHEK1 and BUB1 may play an important role in ovarian cancer. PMID: 23383610
the molecular mechanism of DNA damage-induced Bub1 activation and highlight a critical role of Bub1 in DNA damage response PMID: 22071147
ATM-mediated Bub1 Ser314 phosphorylation is required for Bub1 activity and is essential for the activation of the spindle checkpoint. PMID: 22099307
Bub1 expression could be regulated by estradiol and paclitaxel in endometrial carcinoma Ishikawa cells. PMID: 21092550
Results establish that Bub1 has oncogenic properties and suggest that Aurora B is a critical target through which overexpressed Bub1 drives aneuploidization and tumorigenesis. PMID: 21646403
Findings strongly suggest that the loss of spindle assembly checkpoint proteins, such as Bub1 and Mad2, may cause spontaneous miscarriages. PMID: 20643875
h-KNL1 functions to connect Bub1 and BubR1 with the hMis12, Ndc80, and Zwint-1 complexes. PMID: 21199919
findings show phosphorylation of histone H3-threonine 3 mediated by Haspin cooperates with Bub1-mediated histone 2A-serine 121 phosphorylation in targeting the chromosomal passenger complex to the inner centromere in fission yeast and human cells PMID: 20929775
the interaction between LANA and the kinetochore proteins CENP-F and Bub1 is important for KSHV genome tethering and its segregation to new daughter cells PMID: 20660191
Sequence alterations of mitotic checkpoint genes, hBUB1 and hBUBR1, were examined, and their gene transcripts were quantified using on-line, real-time quantitative reverse transcription-PCR in surgically resected human colorectal cancers PMID: 11782350
As part of a common complex in checkpoint-activated cells, Bub1 monitors different spindle events compared to BubR1 and integrates different signals into a single signal that is then relayed to the downstream cell cycle machinery. PMID: 11792804
Reduced expression and aberrant transcription of the hBUB1 gene were detected in acute myeloid leukemia specimens. PMID: 11999574
mutations in leukemia and lymphoma cells PMID: 12096343
Results suggest that mutation of the Bub1 gene might not play a role in human stomach and oral carcinogenesis. PMID: 12375025
mitotic checkpoint kinases BubR1 and BuB1, by binding to beta-adaptins, may play novel roles in the regulation of vesicular intracellular traffic PMID: 12419313
The checkpoint pathway can be abrogated by mutations in BUB1 in the pancreatic cancer cell line Hs766T. PMID: 12655561
No apparent selection trend was established with neoplastic progression or aneuploidy for BUB1 in Barrett esophagus. PMID: 15013707
Bub1 protects centromeric cohesion through Shugoshin in mitosis PMID: 15604152

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Subcellular Location

Nucleus. Chromosome, centromere, kinetochore.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family, BUB1 subfamily

Tissue Specificity

High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index.

Database Links

HGNC: 1148

OMIM: 602452

KEGG: hsa:699

STRING: 9606.ENSP00000302530

UniGene: Hs.469649

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Code	CSB-RA281837A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate All lanes: Bub1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 123, 116, 120 kDa Observed band size: 130 kDa
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BUB1 Recombinant Monoclonal Antibody

Product Details

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Customer Reviews and Q&A

Target Background