CALM3/CALM2/CALM1 Recombinant Monoclonal Antibody

Calmodulin serves as a master regulator of calcium signaling, mediating the effects of intracellular calcium fluctuations on hundreds of downstream target proteins. The three human calmodulin genes—CALM1, CALM2, and CALM3—encode identical 17 kDa proteins, making this calcium sensor one of the most highly conserved proteins across eukaryotes. Given calmodulin's central role in processes ranging from cell cycle progression to neurotransmission, reliable detection of this ubiquitous signaling hub is essential for researchers investigating calcium-dependent pathways in health and disease.

This recombinant rabbit monoclonal antibody, clone 4D12, offers the reproducibility that calcium signaling studies demand. Because the antibody sequence is defined and production occurs in controlled recombinant systems, researchers can expect consistent performance across experiments and over time—eliminating the lot-to-lot variability that can complicate longitudinal studies or multi-site collaborations.

Validation data demonstrate this antibody's versatility across complementary detection platforms. Immunohistochemistry performed on paraffin-embedded human colorectal cancer tissue reveals clear target localization using citrate-based antigen retrieval, with effective staining at 1:100 dilution. Flow cytometry analysis of MCF-7 breast cancer cells confirms intracellular detection capability, showing distinct positive signal separation from isotype control when cells are fixed and permeabilized prior to staining.

This combination of validated applications provides researchers with flexibility to examine calmodulin expression at both tissue architecture and single-cell resolution levels. The antibody's demonstrated performance in human cancer specimens and cell lines makes it particularly relevant for oncology research, where dysregulated calcium signaling frequently contributes to tumor progression, as well as broader investigations into signal transduction mechanisms.