CDC5L Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA858712A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate All lanes: CDC5L antibody at 1.3μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 93 KDa Observed band size: 100 KDa IHC image of CSB-RA858712A0HU diluted at 1:126.5 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-RA858712A0HU diluted at 1:126.5 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of A549 cells with CSB-RA858712A0HU at 1:42, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). Immunoprecipitating CDC5L in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-RA858712A0HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA858712A0HU (3μg) + Hela whole cell lysate (500μg) Lane 3: Hela whole cell lysate (20μg)
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Product Details
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Target Background

Product Details

Uniprot No.

Q99459

Target Names

CDC5L

Alternative Names

CDC5 antibody; CDC5 cell division cycle 5-like (S. pombe) antibody; Cdc5 related protein antibody; CDC5-LIKE antibody; Cdc5-like protein antibody; Cdc5l antibody; CDC5L_HUMAN antibody; CEF1 antibody; Cell division cycle 5 S. pombe homolog of antibody; Cell division cycle 5-like protein antibody; dJ319D22.1 antibody; KIAA0432 antibody; PCDC5RP antibody; Pombe Cdc5 related protein antibody; Pombe Cdc5-related protein antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human CDC5L

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

1E3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:20-1:200
IP	1:200-1:1000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

CDC5L plays a central role in pre-mRNA splicing as a core component of the Prp19 complex, making it essential for proper gene expression and cell cycle progression. This protein's involvement in spliceosome assembly and activation positions it as a significant target for researchers investigating RNA processing mechanisms, cell division regulation, and the broader landscape of epigenetics and nuclear signaling.

This recombinant monoclonal antibody, generated from clone 1E3 in rabbit host, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined sequences rather than traditional hybridoma methods, you can expect reliable performance across experiments and between lots, eliminating a common source of variability in long-term studies.

Validation data demonstrates robust performance across multiple applications. In western blotting, the antibody detects CDC5L across a diverse panel of human cell lines including HeLa, MCF-7, HepG2, 293, K562, and Jurkat lysates at dilutions ranging from 1:500 to 1:5000. The observed band at approximately 100 kDa runs slightly higher than the predicted 93 kDa molecular weight, which likely reflects post-translational modifications such as phosphorylation, a common feature of proteins involved in cell cycle regulation. Immunohistochemistry validation in human appendix and pancreatic tissues confirms utility for tissue-based studies, while immunofluorescence staining in A549 cells reveals the expected nuclear localization pattern. The antibody also performs effectively in immunoprecipitation assays using HeLa lysates, enabling protein interaction studies.

Whether you're investigating splicing factor dynamics, characterizing nuclear protein complexes, or exploring CDC5L's role in cellular proliferation, this antibody provides the versatility and validation depth needed for confident results.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

DNA-binding protein involved in cell cycle control. May act as a transcription activator. Plays role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR).

Gene References into Functions

Study demonstrates that the levels of Prp19 and Cdc5L are overexpressed in hepatocellular carcinoma (HCC). Prp19 binds with Cdc5L and its downregulation results in reduction of Cdc5L. Mechanistic insights reveal that silencing Prp19 compromises translation activity of Cdc5L and facilitates lysosome-induced degradation of Cdc5L in HCC cells. PMID: 28387715
CDC5L might play an important role in glioma cell survival and disease progression. PMID: 26490980
Cdc5L was highly expressed in hepatocellular carcinoma. Overexpression of Cdc5L favors cell cycle progress of HCC cells, while downregulation of Cdc5L results in cell cycle arrest at G2/M phase and reduced cell proliferation of HCC cells. PMID: 26553251
The results show that CTNNBL1 enhances the association of CWC15 and CDC5L, both core Prp19 complex proteins and identify an overlap in the region of CDC5L that binds either CTNNBL1 or CWC15 suggesting the two proteins might exchange places in the complex. PMID: 26130721
Cdc5L is a key regulator of mitotic progression. PMID: 24675469
The N-terminal ARM-repeat domain of CTNNBL1 provides a binding site for CDC5L, a binding partner in the Prp19-CDC5L complex. PMID: 24598747
CTNNBL1 is a novel nuclear localization sequence-binding protein that recognizes RNA-splicing factors CDC5L and Prp31 PMID: 21385873
Dbf4 regulates the Cdc5 Polo-like kinase through a distinct non-canonical binding interaction PMID: 21036905
A central region in hnRNP-M is required for interaction with CDC5L/PLRG1. PMID: 20467437
Blom7alpha is a novel splicing factor of the K homology domain family that might be implicated in alternative splicing by helping to position the CDC5L-SNEV(Prp19-Pso4) complex at the splice sites PMID: 19641227
CDC5 may function in pre-mRNA processing and cell cycle progression PMID: 14515018
the interaction between CDC5L and PLRG1 is essential for pre-mRNA splicing PMID: 14576297
Results indicate that CDC5L is the most likely candidate oncogene for the 6p12-p21 amplicon found in osteosarcoma. PMID: 18567798
Phosphorylation of CDC5L at threonines 411 and 438 within recognition sequences for CDKs are required for CDC5L-mediated pre-mRNA splicing. PMID: 18583928

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Involvement in disease

A chromosomal aberration involving CDC5L is found in multicystic renal dysplasia. Translocation t(6;19)(p21;q13.1) with USF2.

Subcellular Location

Nucleus. Nucleus speckle. Cytoplasm.

Protein Families

CEF1 family

Tissue Specificity

Ubiquitously expressed in both fetal and adult tissues.

Database Links

HGNC: 1743

OMIM: 602868

KEGG: hsa:988

STRING: 9606.ENSP00000360532

UniGene: Hs.485471

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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