Home
Antibody
Recombinant Antibodies
COL6A1/COL6A2/COL6A3 Recombinant Monoclonal Antibody

COL6A1/COL6A2/COL6A3 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA964035A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA964035A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA964035A0HU diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing 786-O cells stained with CSB-RA964035A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Customer Reviews and Q&A

Product Details

Uniprot No.

P12109 P12110 P12111

Target Names

COL6A1/COL6A2/COL6A3

Alternative Names

Alpha 1 (VI) chain (61 AA) antibody; CO6A1_HUMAN antibody; COL6A1 antibody; COL6A2 antibody; COL6A3 antibody; collagen 6 antibody; Collagen alpha 2(VI) chain antibody; Collagen alpha 3(VI) chain antibody; Collagen alpha-1(VI) chain antibody; collagen six antibody; Collagen type VI alpha 1 antibody; Collagen type VI alpha 2 antibody; Collagen type VI alpha 3 antibody; Collagen VI alpha 1 polypeptide antibody; Collagen VI alpha 2 polypeptide antibody; Collagen VI alpha 3 polypeptide antibody; CollagenVI antibody; Human mRNA for collagen VI alpha 2 C terminal globular domain antibody; OPLL antibody; PP3610 antibody; CO6A2_HUMAN antibody; COL6A2 antibody; Collagen alpha 2(VI) chain antibody; Collagen alpha-2(VI) chain antibody; collagen type VI alpha 2 antibody; Collagen VI alpha 2 polypeptide antibody; human mRNA for collagen VI alpha 2 C terminal globular domain antibody; PP3610 antibody; CO6A3_HUMAN antibody; COL6A3 antibody; Collagen alpha-3(VI) chain antibody; Collagen type VI alpha 3 antibody; Collagen VI alpha 3 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human COL6A1/COL6A2/COL6A3 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

15C5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address