COPS3 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA556985A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: U251 whole cell lysate, HEK293 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, HL-60 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate All lanes: COPS3 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 48, 46 kDa Observed band size: 40-55 kDa IHC image of CSB-RA556985A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA556985A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of A549 cell with CSB-RA556985A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 535-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing MCF7 cells stained with CSB-RA556985A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

Q9UNS2

Target Names

COPS3

Alternative Names

COP9 signalosome complex subunit 3 (SGN3) (Signalosome subunit 3) (JAB1-containing signalosome subunit 3), COPS3, CSN3

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human COPS3

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

3F9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

To produce the COPS3 recombinant monoclonal antibody, a series of complex processes is required. First, the COPS3 monoclonal antibody is collected and its gene sequence is determined. A COPS3 monoclonal antibody gene-carrying vector is then constructed and introduced into a host cell line for culture. A synthesized peptide from human COPS3 is used as an immunogen during COPS3 monoclonal antibody production. Affinity chromatography is employed to purify the resulting COPS3 recombinant monoclonal antibody, which is then evaluated for specificity using multiple applications, including ELISA, WB, IHC, IF, and FC.

The COPS3 recombinant monoclonal antibody can react with human COPS3 protein.COPS3, also known as CSN3, is a component of the COP9 signalosome complex (CSN) which is a highly conserved protein complex found in eukaryotic cells. The CSN is involved in the regulation of various cellular processes such as protein degradation, cell cycle control, DNA repair, and signal transduction. COPS3 plays a role in the stability and activity of the CSN complex, and its function is essential for the proper function of the complex.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.

Gene References into Functions

Here the authors identify a potential mechanism by which desmosomes assist the de-neddylating COP9 signalosome (CSN) in attenuating EGFR through an association between the Cops3 subunit of the CSN and desmosomal components, Desmoglein1 (Dsg1) and Desmoplakin (Dp), to promote epidermal differentiation. PMID: 28891468
this study demonstrates Csn3 as an oncogene that regulates the tumorigenesis process in hepatocellular carcinoma (HCC) cells. PMID: 22237956
of the COPS3 gene might have important roles in metastasis of osteosarcoma cells. PMID: 21436869

Subcellular Location

Cytoplasm. Nucleus.

Protein Families

CSN3 family

Tissue Specificity

Widely expressed. Expressed at high level in heart and skeletal muscle.

Database Links

HGNC: 2239

OMIM: 604665

KEGG: hsa:8533

STRING: 9606.ENSP00000268717

UniGene: Hs.6076

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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