DNPH1 Recombinant Monoclonal Antibody

DNPH1, also known as deoxyribonucleoside 5'-monophosphate N-glycosidase, plays a critical role in nucleotide metabolism and has garnered significant attention as a c-Myc-responsive protein. This connection to Myc signaling pathways positions DNPH1 as a relevant target for researchers investigating cell proliferation, metabolic reprogramming, and oncogenic transformation. Understanding DNPH1 expression and localization can provide valuable insights into how cells manage nucleotide pools under normal and pathological conditions.

This recombinant monoclonal antibody, clone 13H11, offers the reproducibility and consistency that demanding experimental workflows require. Developed using recombinant technology with a defined sequence, this rabbit IgG antibody eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The antibody was raised against a synthetic peptide derived from human DNPH1 and has been validated for cross-species reactivity with both human and mouse samples, enabling comparative studies across these commonly used model systems.

Validation studies demonstrate reliable performance in immunofluorescence and flow cytometry applications. Immunofluorescence staining of NIH/3T3 mouse fibroblasts reveals clear cellular signal when used at dilutions between 1:50 and 1:200, with formaldehyde fixation and Triton X-100 permeabilization. Flow cytometry analysis using HeLa cells confirms specific detection, showing distinct separation between antibody-stained populations and isotype control samples at 1:100 dilution.

Supplied in a glycerol-containing buffer optimized for long-term storage stability, this affinity-purified antibody is well-suited for cell biology research exploring nucleotide metabolism, Myc-driven cellular responses, and related signaling networks.