Code | CSB-RA238318A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:5000 |
CUSABIO's product CSB-RA238318A0HU is prepared using protein and DNA recombinant technology. Initially, mice were immunized with a synthetic peptide from human DOT1L, and after a specific period, the spleen was removed aseptically. The RNA from the spleen cells was isolated, and the cDNA was generated through reverse transcription. The DOT1L antibody gene was amplified via PCR using this cDNA as a template. The obtained gene was inserted into a vector, which was then transfected into host cells for culturing. The DOT1L recombinant monoclonal antibody was purified from the cell culture supernatant using affinity chromatography and has been validated for use in ELISA and WB experiments to detect human DOT1L protein.
The DOT1L protein is specifically involved in the methylation of H3K79 on nucleosomes that contain histone H2B ubiquitinated at lysine 120 (H2Bub1). This modification is associated with the elongation phase of transcription, transcriptional activation, DNA repair, and maintenance of chromatin structure. The activity of DOT1L is thought to contribute to the proper regulation of gene expression during development and differentiation. Mutations in the DOT1L gene have been associated with certain types of leukemia.
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