DPF2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA795437A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Jurkat whole cell lysate(30µg), A431 whole cell lysate(30µg), K562 whole cell lysate(30µg) All lanes: DPF2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time：1min Immunofluorescence staining of U-251MG cell with CSB-RA795437A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing jurkat cells stained with CSB-RA795437A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

Q92785

Target Names

DPF2

Alternative Names

Zinc finger protein ubi-d4 (Apoptosis response zinc finger protein) (BRG1-associated factor 45D) (BAF45D) (D4, zinc and double PHD fingers family 2) (Protein requiem), DPF2, BAF45D REQ UBID4

Species Reactivity

Human

Immunogen

A synthesized peptide from human DPF2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

3E2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

DPF2, also known as BAF45D or Requiem, serves as a critical component of the SWI/SNF chromatin remodeling complex, where it functions as a tissue-specific subunit that helps regulate gene expression programs. This zinc finger protein plays important roles in apoptosis and cellular differentiation, making it a significant target for researchers investigating chromatin biology, transcriptional regulation, and cell fate decisions.

This recombinant monoclonal antibody, clone 3E2, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and produced recombinantly in rabbit, researchers can expect reliable performance across experiments and eliminate the lot-to-lot variability that often complicates long-term studies. The affinity-purified IgG format ensures high specificity for your target while minimizing background.

Validation across multiple platforms demonstrates this antibody's versatility in your research toolkit. Western blot analysis confirms detection of DPF2 at the expected 44 kDa molecular weight in human cell lines including Jurkat, A431, and K562, with recommended dilutions ranging from 1:500 to 1:2000. For cellular localization studies, immunofluorescence staining in U-251MG glioblastoma cells reveals clear signal when used at 1:50 to 1:200 dilutions. Flow cytometry applications have been validated in Jurkat cells, enabling quantitative analysis of DPF2 expression at the single-cell level.

Whether you are exploring SWI/SNF complex function, investigating chromatin remodeling mechanisms, or studying apoptotic pathways, this antibody provides a dependable tool for detecting endogenous DPF2 in human samples across multiple experimental approaches.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Plays an active role in transcriptional regulation by binding modified histones H3 and H4. Is a negative regulator of myeloid differentiation of hematopoietic progenitor cells. Might also have a role in the development and maturation of lymphoid cells. Involved in the regulation of non-canonical NF-kappa-B pathway.

Gene References into Functions

de novo variants in DPF2 cause Coffin-Siris syndrome and propose a dominant-negative mechanism of pathogenicity. PMID: 29429572
Authors show that DPF2 interacts with the acetylated tails of both histones 3 and 4 via bipartite binding pockets on the DPF2 surface. Blocking these interactions through targeted mutagenesis of DPF2 abolishes its recruitment to target chromatin regions as well as its ability to prevent myeloid differentiation in vivo. PMID: 28533407
Upon influenza virus infection, DPF2 dysregulated IFN-beta induction and expression of cytokines/chemokines and antiviral proteins. This study provides evidence that influenza virus utilizes DPF2 to escape host innate immunity. PMID: 28404846
The demonstrate that the histone acetylation-binding double PHD finger (DPF) domains of human MOZ (also known as KAT6A) and DPF2 (also known as BAF45d) accommodate a wide range of histone lysine acylation with the strongest preference for propionylation (Kcr). PMID: 27775714
the role of SMARCA4 and the two SWI/SNF subunits SMARCD2/BAF60B and DPF2/BAF45D in leukaemia, was investigated. PMID: 26571505
DPF2 decreases monomeric and mono-ubiquitinated OCT4, assembles poly-ubiquitin chains on OCT4 mainly through Ub-K48 linkage PMID: 26417682
crystal structure of the C2H2-type zinc finger domain of human DPF2 with a canonical C2H2 fold, which contains two beta strands and one alpha helix, was reported. PMID: 21888896
found that targeting Requiem and Alg-2 did not result in extended culture viability, but resulted in an increase in maximum viable cell numbers and cumulative IVCD under fed-batch conditions PMID: 20571937

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Subcellular Location

Nucleus. Cytoplasm.

Protein Families

Requiem/DPF family

Tissue Specificity

Ubiquitous.

Database Links

HGNC: 9964

OMIM: 601671

KEGG: hsa:5977

STRING: 9606.ENSP00000436901

UniGene: Hs.13495

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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