DSTN Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA440534A0HU
Size	US$210
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Image	IHC image of CSB-RA440534A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA440534A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing Raji cells stained with CSB-RA440534A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

P60981

Target Names

DSTN

Alternative Names

2610043P17Rik antibody; ACTDP antibody; Actin depolymerizing factor antibody; Actin-depolymerizing factor antibody; ADF antibody; AU042046 antibody; bA462D18.2 antibody; corn1 antibody; DEST_HUMAN antibody; Destrin (actin depolymerizing factor) antibody; Destrin antibody; DSN antibody; Dstn antibody; Epididymis luminal protein 32 antibody; HEL32 antibody; Sid 23 antibody; sid23p antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human DSTN

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

15B11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Destrin, also known as actin-depolymerizing factor (ADF), plays a fundamental role in regulating actin filament dynamics within cells. By severing and depolymerizing actin filaments, DSTN enables the rapid cytoskeletal remodeling essential for cell motility, division, and morphological changes. This makes destrin a protein of significant interest in cancer research, developmental biology, and studies of cellular mechanics, where understanding actin dynamics provides critical insights into disease progression and normal physiological processes.

This recombinant monoclonal antibody against human DSTN offers the consistency and reliability that demanding research protocols require. Developed using recombinant technology and derived from a single clone (15B11), this antibody provides sequence-defined specificity with minimal lot-to-lot variation, ensuring your experimental results remain reproducible across extended studies. The rabbit IgG format and affinity-chromatography purification deliver a reagent optimized for sensitive detection applications.

Validation studies demonstrate robust performance across multiple experimental platforms. Immunohistochemistry testing in paraffin-embedded human colorectal cancer and kidney tissues at 1:100 dilution reveals clear cytoplasmic staining patterns consistent with destrin's known cellular localization, using standard citrate buffer antigen retrieval protocols on automated Leica Bond systems. Flow cytometry analysis using Raji cells confirms the antibody's utility for single-cell analysis of intracellular destrin expression, with clear population separation demonstrated at 1:100 dilution following formaldehyde fixation and Triton X-100 permeabilization.

Whether investigating actin dynamics in tumor microenvironments, examining cytoskeletal regulation in normal tissue architecture, or characterizing destrin expression at the single-cell level, this antibody provides a dependable tool for advancing your research into cellular mechanics and motility.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Actin-depolymerizing protein. Severs actin filaments (F-actin) and binds to actin monomers (G-actin). Acts in a pH-independent manner.

Gene References into Functions

analysis of human Cof1, Cof2, and ADF effects on actin filament severing and turnover PMID: 26996939
Destrin is upregulated in nerve-invasive pancreatic cancer cells and its expression might be related to perineural invasiveness PMID: 22898637
The ADF/cofilin1-dependent severing of actin filaments exposes and promotes the activation of SPCA1, which pumps Ca(2+) into the lumen of the TGN for the sorting of the class of secretory cargo that binds Ca(2+). PMID: 21571222
Changes in the expression of cytoskeletal regulatory proteins such as LIMK and cofilin may play a role in weakening thoracic aortic medial tissue, as a precondition to thoracic aortic dissection. PMID: 20873970
The results of this study suggested that temporally regulated ADF/cofilin activities function in postsynaptic modifications of receptor number and spine size during synaptic plasticity. PMID: 20835250
differences in actin binding by human ADF and cofilin PMID: 11812157
Important sequence differences between actin-depolymerizing factor/cofilin were correlated with unique structural determinants in the F-actin-binding site to account for differences in biochemical activities of the two proteins. PMID: 14627701
downregulated following UV exposure in epidermis PMID: 15816841
In the absence of any crystal structures of ADF or cofilin in complex with actin, these studies provide further information about the binding sites on F-actin for these important actin regulatory proteins. PMID: 17196218
destrin is a significant regulator of various processes important for invasive phenotype of human colon cancer Isreco1 cells whereas cofilin-1 may be involved in only a subset of them PMID: 17583572

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Protein Families

Actin-binding proteins ADF family

Tissue Specificity

Widely distributed in various tissues.

Database Links

HGNC: 15750

OMIM: 609114

KEGG: hsa:11034

STRING: 9606.ENSP00000246069

UniGene: Hs.304192

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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