Product Details

Uniprot No.

Target Names

EIF2B5

Alternative Names

CACH; CLE; EI2BE_HUMAN; EIF 2B; eIF 2B GDP GTP exchange factor; eIF 2B GDP GTP exchange factor subunit epsilon; eIF-2B GDP-GTP exchange factor subunit epsilon; EIF2B; EIF2B5; EIF2BE; EIF2Bepsilon; Eukaryotic Translation Initiation Factor 2 Beta Epsilon; Eukaryotic translation initiation factor 2B subunit 5 epsilon 82kDa; Eukaryotic translation initiation factor 2B subunit 5 epsilon; LVWM; Translation initiation factor eIF 2B subunit epsilon; Translation initiation factor eIF-2B subunit epsilon

Species Reactivity

Human

Immunogen

A synthesized peptide from human EIF2B5 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

1G9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, FC

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Protocols

ELISA Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

EIF2B5 encodes the epsilon subunit of eukaryotic translation initiation factor 2B, a guanine nucleotide exchange factor essential for protein synthesis initiation. This catalytic subunit plays a central role in regulating global translation rates in response to cellular stress, making it a critical target for researchers investigating translational control mechanisms. Mutations in EIF2B5 are associated with vanishing white matter disease, also known as childhood ataxia with central nervous system hypomyelination, positioning this protein as an important focus in neurological disorder research.

This recombinant monoclonal antibody, clone 1G9, offers the reproducibility and consistency that demanding experimental workflows require. Produced using recombinant technology in rabbit host, the antibody provides sequence-defined specificity against human EIF2B5, ensuring reliable performance across experiments and eliminating the lot-to-lot variability that can compromise longitudinal studies. The monoclonal nature guarantees recognition of a single epitope, generated against a synthesized peptide from the human EIF2B5 protein.

Validation through flow cytometry demonstrates clear detection of EIF2B5 in HeLa cells, with recommended dilutions ranging from 1:50 to 1:200. The flow cytometry data shows distinct separation between antibody-stained and control populations, confirming specific intracellular detection following fixation and permeabilization. The antibody is also validated for ELISA applications, providing flexibility for researchers employing multiple detection platforms.

Supplied in a glycerol-containing buffer optimized for long-term stability at -20°C or -80°C, this unconjugated antibody is compatible with standard secondary detection systems. Researchers studying translational regulation, integrated stress response pathways, or leukodystrophy mechanisms will find this antibody a dependable tool for characterizing EIF2B5 expression and function.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.

Gene References into Functions

Exon-level analyses classified > 1,000 mRNAs as alternatively spliced under hypoxia and uncovered a unique retained intron (RI) in the master regulator of translation initiation, EIF2B5. PMID: 28961236
This study demonstrated that no evidence for a role of Ile587Val polymorphism of EIF2B5 gene in multiple sclerosis in Kashmir Valley if India. PMID: 26671108
Patient exhibits an early-infantile onset and progressive disease course resembling Cree leukoencephalopathy, suggesting a severe functional disruption of eIF2Bepsilon caused by R195H as well as by I408T mutations. PMID: 25457085
Missense mutations in EIF2B5 are associated with multiple sclerosis. PMID: 24980014
Also a mutation c.1913G>A[p.Arg638 His] in exon 14 of the EIF2B5-Gens as single nucleotide polymorphism. PMID: 21503715
crystal structure of the guanine nucleotide exchange factor for elf2 PMID: 21204011
Functional analysis of recently identified mutations in eukaryotic translation initiation factor 2Bepsilon (eIF2Bepsilon) identified in Chinese patients with vanishing white matter disease PMID: 21307862
The clinical phenotype in vanishing white matter disease is influenced by the combination of both mutations(p.Arg113His and p.Thr91Ala/p.Arg339any). Females tend to do better than males. PMID: 20975056
Cree leukoencephalopathy and CACH/VWM disease are allelic at the EIF2B5 locus. PMID: 12325082
Mutation in EIF2B5 causes childhood ataxia with central nervous system hypomyelination/ vanishing white matter leukodystrophy. PMID: 12707859
We demonstrated that mutations in the gene EIF2B5 cause "leukoencephalopathy with vanishing white matter." This gene encodes one of the five subunits of the translation factor eIF2B. PMID: 14572143
mutations in EIF2b5 causes a specific reduction in the generation of GFAP+ astrocytes in vanishing white matter disease PMID: 15723074
Arg113His mutation in eIF2B5 associated with adult onset of vanishing white matter leukoencephalopathy is not present in multiple sclerosis patients PMID: 17439913
We describe progressive megalencephaly and severe brain abnormalities due to specific EIF2Bepsilon mutations in two unrelated families. PMID: 17646634
These data support the importance of the non-catalytic domain of the eIF2Bepsilon subunit in the eIF2B complex formation and activity. PMID: 18294360
no evidence for the involvement of EIF2B5 in multiple sclerosis susceptibility in France PMID: 18562513
Resistance exercise decreases eIF2Bepsilon phosphorylation and potentiates the feeding-induced stimulation of p70S6K1 and rpS6 in young men. PMID: 18565837

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Involvement in disease

Leukodystrophy with vanishing white matter (VWM)

Protein Families

EIF-2B gamma/epsilon subunits family

Database Links

HGNC: 3261

OMIM: 603896

KEGG: hsa:8893

STRING: 9606.ENSP00000273783

UniGene: Hs.283551

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Code	CSB-RA945280A0HU
Size	US$210
	Order now
Image	Overlay Peak curve showing Hela cells stained with CSB-RA945280A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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EIF2B5 Recombinant Monoclonal Antibody

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Target Background