Product Details

Uniprot No.

Target Names

Alternative Names

Eukaryotic translation initiation factor 5A-1 (eIF-5A-1) (eIF-5A1) (Eukaryotic initiation factor 5A isoform 1) (eIF-5A) (Rev-binding factor) (eIF-4D), EIF5A

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human eIF5A

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

5E1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:20-1:200
FC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Eukaryotic translation initiation factor 5A (EIF5A) stands as the only known protein to undergo hypusination, a unique post-translational modification essential for its function in translation elongation and termination. This distinctive biochemistry makes EIF5A a compelling target in studies of protein synthesis regulation, cell proliferation, and increasingly, cancer biology, where its expression patterns have drawn significant research attention.

This recombinant monoclonal antibody, clone 5E1, offers the reproducibility that demanding experimental workflows require. Generated through recombinant technology with a defined sequence, it eliminates the lot-to-lot variability inherent in traditional hybridoma production, ensuring your results remain consistent across extended studies and between laboratory groups.

Validation across multiple platforms demonstrates genuine experimental flexibility. Western blot analysis confirms reliable detection in diverse human cell lines including A2780 ovarian cancer cells, HeLa cervical carcinoma, MCF-7 breast cancer, and lymphoid lines Jurkat and Raji, with an observed band at approximately 18 kDa. The slight difference from the predicted molecular weight likely reflects post-translational modifications characteristic of this heavily modified protein. Immunohistochemistry testing in paraffin-embedded human colon cancer tissue shows clear staining with citrate buffer antigen retrieval, while immunofluorescence in HeLa cells and flow cytometry analysis of Jurkat cells further confirm the antibody's versatility for both fixed-cell imaging and quantitative single-cell applications.

For researchers investigating translational control mechanisms, epigenetic signaling pathways, or the role of EIF5A in disease contexts, this antibody provides a dependable tool with documented performance across the techniques most commonly employed in these fields.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.

Gene References into Functions

EIF5A1 is a vital regulator of epithelial ovarian cancer proliferation and progression. PMID: 29428664
Molecular structure of the exportin Xpo4 in complex with RanGTP and the hypusine-containing translation factor eIF5A has been reported. PMID: 27306458
the depletion of eIF5A leads to endoplasmic reticulum stress, an unfolded protein response and up-regulation of chaperone expression in HeLa cells. PMID: 27180817
Findings indicate that eIF5A-PEAK1-YAP signaling contributes to PDAC development by regulating an STF program associated with increased tumorigenicity. PMID: 28381547
eIF5A1 isoform A has a role in mitochondrial function. PMID: 27414022
eIF5A-2 protein was highly expressed in gastric cancer tissues PMID: 26282002
Upregulation of translation initiation factor 5a observed in thalassemia is a novel finding and plays a protective role toward cell survival under oxidative stress. PMID: 26403856
Our results suggest that functional, hypusinated eIF5A is necessary for HIF-1alpha expression during hypoxia and that eIF5A is an attractive target for cancer therapy. PMID: 26773503
findings also implicate the eIF5A/RhoA/ROCK module as a potential new therapeutic target to treat metastatic PDAC cells PMID: 26483550
eIF5A has been shown to regulate a number of gene products specifically, termed the eIF5A regulon, and its role in translating proline-rich sequences has recently been identified. PMID: 25979826
eIF5A proteins utilize PEAK1 as a downstream effector to drive pancreatic ductal adenocarcinoma (PDAC) pathogenesis. PMID: 25261239
Mature eIF5A (hypusinated form) is not involved in the autophagic pathway. PMID: 25218134
The findings that eIF5A and EF-P are important for specific cellular processes and play a role in the relief of ribosome stalling caused by specific amino acid sequences PMID: 24402910
Data suggest a regulatory mechanism for the pro-apoptotic protein eukaryotic translation initiation factor 5A1 (eIF5A1) in which its level is possibly modulated by NF-kappaB in lung cells. PMID: 24491565
Data indicate that the signal of EIF5A2, MYCN, and MCL1 genes is decreased in hydroxyurea (HU) and gemcitabine (GEM) treated UACC-1598 ovarian cancer cell line. PMID: 23991020
mature eIF5A controls a translational network of cancer-driving genes, termed the eIF5A regulon, at the levels of mRNA abundance and translation PMID: 24220243
The expression of eIF5A-2 was up-regulated following EMT phenotype changes in A549 cells, which correlated with enhanced tumor invasion and metastatic capabilities. PMID: 23733422
Altered expression of Hsc70 and eIF5A-1 may cause defects in nucleocytoplasmic transport and play a role in esophageal carcinogenesis. PMID: 23539416
Overexpression of EIF5A is associated with early-onset colorectal cancer. PMID: 23322277
acetylation regulates the subcellular localization of eIF5A2 PMID: 23132580
a novel role for miR-331-3p and miR-642-5p in the control of prostate cancer cell growth via the regulation of DOHH expression and eIF5A activity. PMID: 22908221
identified PCAF as the major cellular acetyltransferase of eIF5A, and HDAC6 and SIRT2 as its major deacetylases. Inhibition of the deacetylases or impaired hypusination increased acetylation of eIF5A, leading to nuclear accumulation. PMID: 22771473
HIV-1 Rev cofactors Sam68, eIF5A, hRIP, and DDX3 also function in the translation of HIV-1 RNA. PMID: 21360055
hybrid exercise increases expression of eukaryotic translation initiation factor 5A (EIFSA), peroxisomal biogenesis factor 6 (PEX6) and histone cluster 1 H4 (HIST1H4), compared with electrical stimulation alone PMID: 21778671
findings suggested that overexpression of eIF5A-2 correlates with local invasion of NSCLC, and might serve as an adverse prognostic marker of survival for stage I NSCLC patients PMID: 20830705
loss of eIF5A activity by this SSAT1-mediated acetylation confirms the strict structural requirement for the hypusine side chain and suggests a possible regulation of eIF5A by hypusine acetylation/deacetylation PMID: 20942800
High eIF5A expression is associated with hepatocellular carcinoma. PMID: 19998337
These observations collectively indicate that unhypusinated eIF5A1 plays a central role in the regulation of apoptosis. PMID: 20232312
These data support the importance of eIF5A and hypusine formation in HIV-1 gene expression. PMID: 19825182
Heat stress-induced loss of eukaryotic initiation factor 5A (eIF-5A) in a human pancreatic cancer cell line PMID: 11870779
Subcellular distribution of eIF-5A by indirect immunofluorescent staining and by direct visualization of green fluorescent protein tagged eIF-5A (GFP-eIF5A) PMID: 12210765
Patients having a higher eIF-5A protein expression showed a relatively poorer survival suggesting the use of eIF-5A as prognostic marker in lung adenocarcinoma. PMID: 12687616
EIF-5A expression inhibition by antisense oligodeoxynucleotides significantly enhanced the stimulating effect of GM-CSF on cell growth. Therefore, the eIF-5A accumulation played important roles in the apoptosis induced by UP inhibitors. PMID: 12894223
eIF5A may be a regulator of p53, and syntenin might regulate p53 by balancing the regulation of eIF5A signaling to p53 for apoptosis PMID: 15371445
These findings suggest that the failure to detect eIF5A-2 protein even in eIF5A-2 mRNA positive cells is, at least in part, due to inefficient translation. PMID: 16519677
Recombinant human eIF-5A was crystallized by the hanging-drop vapor diffusion method PMID: 16522190
molecular model for the human eIF5A protein based on the crystal structure of the eIF5A from Leishmania brasiliensis PMID: 16842744
data suggest that eIF5A mediates important cellular processes like cell viability and senescence through its effects on the stability of certain mRNAs PMID: 16987817
analysis of differences in global protein expression in BCR-ABL-positive K562 cells treated with or without imatinib revealed down-regulation of eIF5A; hypusination inhibitors exerted an antiproliferative effect PMID: 17008552
These findings collectively indicate that unhypusinated eIF5A may have pro-apoptotic functions and that eIF5A is rapidly translocated to the nucleus following the induction of apoptotic cell death PMID: 17187778
analysis of the deoxyhypusine hydroxylase-eukaryotic translation initiation factor (eIF5A) interaction PMID: 17213197
Mutational analyses of human eIF5A-1--identification of amino acid residues critical for eIF5A activity and hypusine modification PMID: 18067580
Results suggest that the stability of eIF5A may have an important role in determining the fate of the particular cell type after severe heat stress. PMID: 19160416
These findings provide strong evidence that the hypusine modification of eIF5A dictates its localization in the cytoplasmic compartment where it is required for protein synthesis. PMID: 19379712
Eukaryotic initiation factor 5A (eIF5A) (eIF-4D, eIF-5A) stimulates ribosomal peptidyltransferase activity, transport of HIV-1-mRNAs and binds exportins 1 and 4. Contains hypusine at lys 50. Human EIF5A1 and EIF5A2 encode two isoforms: eIF5AI and eIF5AII. PMID: 11161802

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Subcellular Location

Cytoplasm. Nucleus. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus, nuclear pore complex. Note=Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.

Protein Families

EIF-5A family

Tissue Specificity

Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).

Database Links

HGNC: 3300

OMIM: 600187

KEGG: hsa:1984

STRING: 9606.ENSP00000336702

UniGene: Hs.104825

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Code	CSB-RA932542A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: A2780 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate All lanes: EIF5A antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 17, 21 kDa Observed band size: 18 kDa IHC image of CSB-RA932542A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela Cells with CSB-RA932542A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). Overlay histogram showing Jurkat cells stained with CSB-RA932542A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/110⁶ cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/110⁶ cells) used under the same conditions. Acquisition of >10,000 events was performed.
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EIF5A Recombinant Monoclonal Antibody

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Target Background