FDFT1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA909932A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: THP-1 whole cell lysate, K562 whole cell lysate All lanes: FDFT1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 49, 41, 39, 36, 44 kDa Observed band size: 50 kDa IHC image of CSB-RA909932A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA909932A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Target Background

Product Details

Uniprot No.

P37268

Target Names

FDFT1

Alternative Names

Squalene synthase (SQS) (SS) (EC 2.5.1.21) (FPP:FPP farnesyltransferase) (Farnesyl-diphosphate farnesyltransferase), FDFT1

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human FDFT1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

10H12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

FDFT1, also known as squalene synthase, catalyzes the first committed step in cholesterol biosynthesis by converting two molecules of farnesyl pyrophosphate into squalene. This enzymatic checkpoint positions FDFT1 as a critical regulator of sterol metabolism, making it a compelling target for researchers investigating metabolic disorders, cardiovascular disease, and cancer biology, where altered cholesterol synthesis often supports rapid cell proliferation.

This recombinant monoclonal antibody, generated in rabbit against a synthetic peptide derived from human FDFT1, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and production is cell-based, you can expect uniform performance across lots, eliminating the variability that can complicate long-term studies or multi-site collaborations.

Validation data demonstrates reliable detection across multiple platforms. In Western blot applications, the antibody identifies FDFT1 in THP-1 and K562 whole cell lysates at dilutions ranging from 1:500 to 1:5000, producing a clear band at approximately 50 kDa. The slight difference from the predicted molecular weights likely reflects post-translational modifications or the detection of a predominant isoform. For immunohistochemistry, the antibody has been validated in paraffin-embedded human testis and human lung cancer tissue at 1:50 to 1:200 dilutions using citrate buffer antigen retrieval, showing specific staining patterns suitable for tissue-based studies.

Whether you are exploring cholesterol pathway dysregulation in tumor microenvironments, investigating metabolic reprogramming in hematological cell models, or examining FDFT1 expression in cardiovascular or neurological contexts, this antibody provides a dependable tool for both protein quantification and spatial localization studies.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Catalyzes the condensation of 2 farnesyl pyrophosphate (FPP) moieties to form squalene. Proceeds in two distinct steps. In the first half-reaction, two molecules of FPP react to form the stable presqualene diphosphate intermediate (PSQPP), with concomitant release of a proton and a molecule of inorganic diphosphate. In the second half-reaction, PSQPP undergoes heterolysis, isomerization, and reduction with NADPH or NADH to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway.

Gene References into Functions

The results indicate that the ennea-peptide CLSPHSMFC, tetra-peptides SMFC, CKTE, and WHQW can effectively inhibit Squalene Synthase activities (IC50 values equal to 64, 76, 87, and 90 muM, respectively) PMID: 26922723
The minor allele in FDFT1 was associated with advanced fibrosis in the non-steatotic but not in the steatotic subgroup in patients with chronic hepatitis C. PMID: 23870067
Up-regulation of SQS promotes metastasis of lung cancer by enhancing tumor necrosis factor-alpha receptor 1 and nuclear factor-kappaB activation and matrix metallopeptidase 1 expression. PMID: 25152164
Several critical residues that are involved in binding of NADPH have been identified in the active site of squalene synthase. PMID: 24531458
HCV infection increases FDFT1 protein level but not FDFT1 mRNA level. PMID: 24690320
this finding yields a new method of colorimetric screening for the cellular activity of squalene synthases, which are major targets for cholesterol-lowering drugs. PMID: 24333579
ABHD5, PGRMC1 and SQS are novel markers for sebaceous carcinoma and can reliably distinguish sebaceous neoplasms from non-sebaceous tumors, specifically BCC with clear cell features. PMID: 23557589
FDFT1 and its encoded enzyme, squalene synthase, may play an important role in prostate cancer development and its aggressive phenotypes. PMID: 22546838
CTSB and FDFT1 are excluded as candidates for keratolytic winter erythema PMID: 21945151
NASH is intimately related to fibrosis progression, the FDFT1 SNP is a good candidate for a link with inflammation and fibrosis. PMID: 21439984
In multivariate models adjusted for age, body mass index, diabetes, waist/hip ratios, and levels of glycated hemoglobin, the NAFLD activity score was associated with the SNP rs2645424 on chromosome 8 in farnesyl diphosphate farnesyl transferase 1 (FDFT1) PMID: 20708005
Results report the high-resolution x-ray structures of human farnesyl pyrophosphate synthase in complexes with risedronate and zoledronate, two of the leading nitrogen-containing bisphosphonates in clinical use. PMID: 16684881
in prostate cancer cells SQS expression is enhanced by androgens, channeling intermediates of the mevalonate/isoprenoid pathway toward cholesterol synthesis PMID: 17483544
lysine in codon 45 is conserved across 11 mammals and lies in an exonic splicing enhancer site, suggesting this coding variant in the squalene synthase gene influences plasma cholesterol levels, by affecting the intracellular production of cholesterol PMID: 18350552
the liver X receptor alpha directly silencing the expression of two key cholesterologenic enzymes (lanosterol 14alpha-demethylase (CYP51A1), and squalene synthase (farnesyl diphosphate farnesyl transferase 1)) via novel negative LXR DNA response elements PMID: 18676367
squalene synthase may be involved in the etiology of hypercholesterolemia PMID: 19054015
FDFT1 rs7001819 showed no association with obesity, neither when analysing quantitative traits nor when performing case-control studies of obesity PMID: 19245693

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Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Protein Families

Phytoene/squalene synthase family

Tissue Specificity

Widely expressed.

Database Links

HGNC: 3629

OMIM: 184420

KEGG: hsa:2222

STRING: 9606.ENSP00000220584

UniGene: Hs.593928

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