FUBP1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA157765A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Jurkat whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, Raji whole cell lysate, HepG2 whole cell lysate All lanes: FUBP1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 68, 69 kDa Observed band size: 69 kDa IHC image of CSB-RA157765A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA157765A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela Cells with CSB-RA157765A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). Overlay histogram showing Jurkat cells stained with CSB-RA157765A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/110⁶ cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/110⁶ cells) used under the same conditions. Acquisition of >10,000 events was performed. Immunoprecipitating FUBP1 in Jurkat whole cell lysate Lane 1: Rabbit control IgG instead of CSB-RA157765A0HU in Jurkat whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA157765A0HU(2µg)+ Jurkat whole cell lysate(500µg) Lane 3: Jurkat whole cell lysate (10µg)
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Product Details
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Target Background

Product Details

Uniprot No.

Q96AE4

Target Names

FUBP1

Alternative Names

Far upstream element-binding protein 1 (FBP) (FUSE-binding protein 1) (DNA helicase V) (hDH V), FUBP1

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human FUBP1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

7C3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:20-1:200
FC	1:20-1:200
IP	1:200-1:1000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

FUBP1, or Far Upstream Element-Binding Protein 1, serves as a critical transcriptional regulator that binds to the far upstream element of the c-myc promoter, playing essential roles in cell proliferation, differentiation, and apoptosis. This single-stranded DNA-binding protein, which also possesses DNA helicase activity, has emerged as a significant focus in cancer research and epigenetic studies, particularly given its involvement in regulating oncogene expression.

This recombinant monoclonal antibody, generated in rabbit against a synthetic peptide derived from human FUBP1, offers the reproducibility and consistency that demanding research applications require. Because the antibody sequence is defined and produced recombinantly, you can expect reliable performance across experiments and between lots, eliminating the variability often encountered with traditional hybridoma-derived antibodies.

Validation testing demonstrates robust performance across multiple experimental platforms. Western blot analysis detects a clean band at the expected 69 kDa molecular weight across diverse human cell lines including Jurkat, K562, HeLa, Raji, and HepG2, confirming reliable detection in both suspension and adherent cell types. Immunohistochemistry staining has been validated in paraffin-embedded human brain tissue and glioma cancer samples using citrate buffer antigen retrieval, providing researchers studying neural biology or CNS malignancies with a validated detection tool. Immunofluorescence analysis in HeLa cells reveals clear nuclear localization consistent with FUBP1's known function as a transcription factor. Flow cytometry and immunoprecipitation applications have also been validated in Jurkat cells, offering flexibility for researchers investigating FUBP1 protein interactions or expression levels at the single-cell level.

This antibody supports investigations into epigenetics, nuclear signaling, and oncogene regulation where precise FUBP1 detection is essential.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription.

Gene References into Functions

High FUBP1 expression is associated with low Chemosensitivity to Adriamycin in Gastric Cancer. PMID: 28667493
Low FUBP1 expression is associated with adenovirus infection. PMID: 29743362
These results suggest that the interference with the FUBP1/FUSE interaction as a further molecular mechanism that, in addition to the inactivation of TOP1, may contribute to the therapeutic potential of camptothecin/SN-38. PMID: 29031818
The findings demonstrate an association between FUBP1 levels and chordoma progression and prognosis, suggesting that FUBP1 can be used as a biomarker and a potential therapeutic target. PMID: 28780352
we identified cyclin J and far upstream element-binding protein 1 (FUBP1) as novel miR-16 targets, which mediate miR-16 antiproliferative effects. PMID: 27157613
FUBP1 acts as a potential oncogene in clear cell renal cell carcinoma (ccRCC) and may be considered as a novel biomarker or an attractive treatment target of ccRCC. PMID: 28076379
FBP1 expression in Bcell lymphoma was also associated with poor survival outcomes. Functionally, small interfering RNAmediated silencing of FBP1 was able to inhibit the proliferation of Bcell lymphoma cells, resulting in G0/G1 phase cell cycle arrest. PMID: 27599538
FUBP1 may potentially stimulate c-Myc expression in ESCC and its expression may promote esophageal squamous cell carcinoma progression. PMID: 26490982
With the advent of large-scale genome sequencing technology, molecular genetic alterations in FUBP1 promoter have now been identified in the majority of oligodendrogliomas PMID: 26545048
direct connection between the cellular PI3K/AKT/mTOR signaling pathway, frequently activated in human hepatocarcinogenesis, and the enrichment of oncogenic transcription factors of the FBP family PMID: 26901106
Concomitant overexpression of far upstream element (FUSE) binding protein (FBP) interacting repressor (FIR) and its splice variants induce migration and invasion of non-small cell lung cancer cells. PMID: 26177862
FBP1 promotes hepatitis C virus eplication by inhibiting p53 expression. PMID: 25995247
High FBP1 expression was observed in glioma. PMID: 24347226
Apoptosis-mediated cleavage of FBP1 and its decreased expression in epithelial cells induces cell cycle arrest, which may play an important role in colonic epithelial disruption in colitis. PMID: 24966911
We conclude that absent CIC and FUBP1 expressions are potential markers of shorter time to recurrence in oligodendroglial tumors. PMID: 24030748
These findings are the first report describing the regulation of alternative splicing of MDM2 mediated by the oncogenic factor FUBP1. PMID: 24798327
The data indicates an association between FUBP1 expression and proliferation in gliomas. PMID: 24117486
FUBP1 expression differs among gastric tissues; there is a correlation between overall survival rates and age, sex, lymph node metastasis, and distant metastasis. PMID: 24192769
Far upstream element-binding protein 1 and RNA secondary structure both mediate second-step splicing repression. PMID: 23818605
biochemical features of FBP1 PMID: 22926519
Analysis allowed us to define two highly recurrent genetic signatures in gliomas: IDH1/ATRX (I-A) and IDH1/CIC/FUBP1 (I-CF). PMID: 22869205
Found CIC and FUBP1 mutations in oligodendrogliomas and demonstrate the presence of these mutations in oligoastrocytomas. PMID: 22588899
increased polyubiquitination of FBP1 does not alter its protein stability, but instead modulates the stable recruitment of FBP1 to target loci PMID: 21779003
The central domain of FBP1, containing four K homology motifs, was required for p27 5'-UTR RNA binding and the N terminal domain was important for translational activation. PMID: 21855647
CIC gene was mutated in 6 oligodendrogliomas and FUBP1 gene was mutated in 2; 27 additional oligodendrogliomas showed 12 and 3 more tumors with mutations of CIC and FUBP1; results suggest role of these genes in biology and pathology of oligodendrocytes PMID: 21817013
The authors suggest that FUSE binding protein 1 binds with the Japanese encephalitis virus untranslated RNA and functions as a host anti-virus defense molecule by repressing viral protein expression. PMID: 21367899
Authors propose that FBP1 is a key regulator of cell growth and proliferation through its ability to selectively bind the NPM 3' UTR and repress NPM translation. PMID: 20802533
The noncoding strand FUSE recruits an activator FUSE-binding protein (FBP) and a repressor FBP-interacting repressor (FIR) to fine-tune c-myc transcription. PMID: 20420426
Results describe the roles of the FarUpStream Element (FUSE), FUSE Binding Protein (FBP), FBP Interacting Repressor (FIR), and TFIIH in the regulation of c-myc expression. PMID: 16628215
FUBP1 is an authentic substrate of Parkin that might play an important role in development of Parkinson disease pathology along with aminoacyl-tRNA synthetase interacting multifunctional protein type 2 PMID: 16672220
investigate the contributions of FBP's 4 K Homology (KH) domains to sequence selectivity. EMSA and missing contact point analysis revealed that FBP contacts 4 separate patches spanning a large segment of FUSE PMID: 19015535
study found that FBP1 as well as FBP3 are more frequently expressed in prostate and bladder cancer than in renal cancer; in addition, a positive correlation between levels of FBP1, FBP3 and c-Myc was exclusively detectable in renal cell carcinomas PMID: 19087307
oncogenic potential of c-Myc is 'switched off' after apoptosis induction as a consequence of the caspase-mediated cleavage of FBP-1. PMID: 19219071
FBP1 is an important oncoprotein overexpressed in hepatocellular carcinoma that induces tumor propagation through direct or indirect repression of cell cycle inhibitors and proapoptotic target genes. PMID: 19637194

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Subcellular Location

Nucleus.

Database Links

HGNC: 4004

OMIM: 603444

KEGG: hsa:8880

STRING: 9606.ENSP00000359804

UniGene: Hs.567380

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