Product Details

Uniprot No.

Target Names

HIRA

Alternative Names

DGCR1 antibody; DiGeorge critical region gene 1 antibody; HIR antibody; HIR histone cell cycle regulation defective homolog A antibody; HIRA antibody; HIRA protein antibody; HIRA_HUMAN antibody; Histone cell cycle regulator antibody; Protein HIRA antibody; TUP 1 antibody; TUP1 like enhancer of split protein 1 antibody; TUP1-like enhancer of split protein 1 antibody; TUPLE1 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human HIRA protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

16E9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, FC

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Protocols

ELISA Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

HIRA plays a central role in chromatin dynamics as a histone chaperone responsible for replication-independent deposition of histone variant H3.3. This function is essential for transcriptional regulation, cellular senescence, and the maintenance of chromatin integrity at actively transcribed genes and regulatory elements. Originally identified within the DiGeorge syndrome critical region, HIRA has become an important focus for researchers investigating epigenetic mechanisms, developmental biology, and age-related chromatin changes.

This recombinant monoclonal antibody, clone 16E9, offers the consistency and reliability that demanding epigenetic studies require. Developed using recombinant technology with a defined sequence, it eliminates the lot-to-lot variability that can compromise longitudinal experiments or multi-site collaborations. The rabbit host and monoclonal nature ensure high specificity for human HIRA, while affinity chromatography purification delivers a clean reagent suitable for sensitive detection methods.

Validation by flow cytometry demonstrates clear performance in intracellular staining applications. Testing in Jurkat cells, a human T lymphocyte line commonly used in immunological and signaling research, shows distinct positive signal separation from isotype control when used at dilutions between 1:50 and 1:200. The protocol employed standard fixation with formaldehyde and permeabilization with Triton X-100, providing a reliable starting point for researchers establishing their own workflows.

The unconjugated format allows flexibility in secondary antibody selection, accommodating various detection systems and multiplexing strategies. For investigators exploring histone variant dynamics, senescence pathways, or chromatin remodeling mechanisms, this antibody provides a dependable tool for characterizing HIRA expression and localization in human cell systems.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.

Gene References into Functions

These data show that HIRA phosphorylation limits the expression of myogenic genes, while the dephosphorylation of HIRA is required for proficient H3.3 deposition and gene activation, demonstrating that the phosphorylation switch is exploited to modulate HIRA/H3.3-mediated muscle gene regulation during myogenesis. PMID: 27515126
Data show that histone chaperone HIRA co-localizes with viral genomes, binds to incoming viral and deposits histone H3.3 onto these. PMID: 28981850
Chromatin reassembly during double-strand break repair was dependent on the HIRA histone chaperone that is specific to the replication-independent histone variant H3.3 and on CAF-1 that is specific to the replication-dependent canonical histones H3.1/H3.2. PMID: 27269284
RPA, best known for its role in DNA replication and repair, recruits HIRA to promoters and enhancers and regulates deposition of newly synthesized H3.3 to these regulatory elements for gene regulation. PMID: 28107649
H3.Y discriminates between HIRA and DAXX chaperone complexes and reveals unexpected insights into human DAXX-H3.3-H4 binding and deposition requirements. PMID: 28334823
PHB has an unexpected nuclear role in human embryonic stem cells that is required for self-renewal and that it acts with HIRA in chromatin organization to link epigenetic organization to a metabolic circuit. PMID: 27939217
The abnormal lower expression of the HIRA gene in the myocardium may participate in the pathogenesis of Tetralogy of Fallot. PMID: 27748330
These results support a model in which OGT modifies HIRA to regulate HIRA-H3.3 complex formation and H3.3 nucleosome assembly and reveal the mechanism by which OGT functions in cellular senescence. PMID: 27217568
HIRA controls a specialized, dynamic H4K16ac-decorated chromatin landscape in senescent cells and enforces tumor suppression. PMID: 25512559
Mechanistic studies reveal that HIRA accumulates at sites of UVC irradiation upon detection of DNA damage prior to repair and deposits newly synthesized H3.3 histones. This local action of HIRA depends on ubiquitylation events associated with damage recognition. PMID: 24074863
HIRA is required for deposition of histone H3.3 at its binding sites. PMID: 23602572
NHRD domain of UBN1 as being an essential region for HIRA interaction and chromatin organization by the HUCA complex PMID: 22401310
HIRA plays a unique, ASF1a-independent role, which is required for the localization of HP1 PMID: 21347226
Data show that, like HIRA, UBN1, and ASF1a, CABIN1 is involved in heterochromatinization of the genome of senescent human cells. PMID: 21807893
phosphorylation of histone H4 Ser 47 catalyzed by the PAK2 kinase, promotes nucleosome assembly of H3.3-H4 and inhibits nucleosome assembly of H3.1-H4 by increasing the binding affinity of HIRA to H3.3-H4 and reducing association of CAF-1 with H3.1-H4 PMID: 21724829
the N-terminal half of HIRA should contribute positively to the growth rate via up-regulation of a set of cell cycle-related genes, whereas the C-terminal half down-regulated another set of them without exhibiting any effect on the cell growth PMID: 16024922
The N- and C-terminal regions of ASF1a and ASF1b determine the different affinities of these two proteins for HIRA, by contacting regions outside the HIRA B domain. CAF-1 also uses B domain-like motifs for binding to ASF1a, thereby competing with HIRA. PMID: 16980972
Hpc2-related domain of UBN1, UBN2, and Hpc2p is an evolutionarily conserved HIRA/Hir-binding domain, which directly interacts with the N-terminal WD repeats of HIRA/Hir. PMID: 19029251
FISH analysis of metaphases revealed a duplication of TUPLE1 probe on one chromosome 22q (Fig. 1). PMID: 19046189

Hide All

Subcellular Location

Nucleus. Nucleus, PML body. Note=Primarily, though not exclusively, localized to the nucleus. Localizes to PML bodies immediately prior to onset of senescence.

Protein Families

WD repeat HIR1 family

Tissue Specificity

Expressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart, liver, lung, and placenta.

Database Links

HGNC: 4916

OMIM: 600237

KEGG: hsa:7290

STRING: 9606.ENSP00000263208

UniGene: Hs.474206

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Code	CSB-RA103196A0HU
Size	US$210
	Order now
Image	Overlay Peak curve showing jurkat cells stained with CSB-RA103196A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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HIRA Recombinant Monoclonal Antibody

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Target Background