Product Details

Uniprot No.

Target Names

HMGA1

Alternative Names

High mobility group protein HMG-I/HMG-Y (HMG-I(Y)) (High mobility group AT-hook protein 1) (High mobility group protein A1) (High mobility group protein R), HMGA1, HMGIY

Species Reactivity

Human

Immunogen

A synthesized peptide from human HMGA1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

1C6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

HMGA1, also known as High Mobility Group AT-hook protein 1, serves as a critical architectural transcription factor that modulates chromatin structure and gene expression. This non-histone chromosomal protein binds to AT-rich regions in DNA and plays significant roles in cell proliferation, differentiation, and transformation. HMGA1 overexpression has been documented across numerous malignancies, making it a compelling target for cancer biology research and studies investigating transcriptional regulation mechanisms.

This recombinant monoclonal antibody, clone 1C6, offers the reproducibility and consistency that demanding experimental workflows require. Generated against a synthetic peptide derived from human HMGA1, the antibody undergoes affinity chromatography purification to ensure high specificity. Because recombinant production relies on defined genetic sequences rather than traditional hybridoma methods, researchers benefit from lot-to-lot consistency that supports longitudinal studies and reproducible results across experiments.

Validation studies demonstrate robust performance across multiple detection platforms. Immunohistochemistry applications have been confirmed in paraffin-embedded human tissues, including pancreatic cancer and small intestine sections, with optimal results achieved at 1:100 dilution using citrate buffer antigen retrieval. Immunofluorescence staining in A431 cells reveals clear nuclear localization patterns consistent with HMGA1's known function as a chromatin-associated protein. Flow cytometry analysis using BxPC-3 pancreatic cancer cells shows distinct positive signal separation from isotype controls, confirming utility for quantitative single-cell studies.

This antibody supports researchers investigating chromatin dynamics, transcriptional regulation, and oncogenic mechanisms. Its validated performance in both tissue sections and cell-based assays provides flexibility for studies examining HMGA1 expression patterns in cancer progression, developmental biology, and cellular transformation pathways.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double-stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.

Gene References into Functions

Study reveals HMGA1, a CK2 Substrate, as a drug-resistant target in non-small cell lung cancer (NSCLC). Results delineate the tyrosine kinase inhibitors (TKI) resistance-associated kinase-substrate network, suggesting a potential therapeutic strategy for overcoming TKI-induced resistance in NSCLC. PMID: 28290473
HMGA1 overexpression increased FoxO1 mRNA and protein expression in vitro, in cultured HepG2 and HEK-293 cells by binding FoxO1 gene promoter, thereby activating FoxO1 gene transcription. PMID: 29052178
High HMGA1 expression is associated with Esophageal Squamous Cell Carcinoma. PMID: 27882471
The HMGA1 rs139876191 variant played a key role in a protective mechanism against proliferative diabetic retinopathy by downregulating the expression of vascular endothelial growth factor A (VEGFA), a major activator of neovascularization in diabetic retinopathy. PMID: 27991577
results from Transwell and woundhealing experiments indicated that HMGA1 participates cell invasion and migration through the ILK/Akt/GSK3beta pathway PMID: 29152644
Thrombocytosis was more prevalent in patients with inflammatory breast cancer (IBC)than in those with non-IBC and it was associated with poor prognosis. GRO and TGF-beta were associated with thrombocytosis in IBC. PMID: 28831670
Findings demonstrate that proper subcellular localization of High-mobility group A1 (HMGA1) is important for its function in trophoblast cells, and suggest that aberrant cytoplasmic expression of HMGA1 contributes to the pathogenesis of preeclampsia through impairment of trophoblast migration. PMID: 28345487
these results clearly indicate HMGA1 as a key regulator of the autophagic pathway in cancer cells. PMID: 28777374
IGF2BP2 protein is a tumor promoter that drives cancer proliferation through its client mRNAs IGF2 and HMGA1. PMID: 28753127
IL-24 inhibits AKT via regulating the HMGA1/miR-222 signaling node in human lung cancer cells and acts as an effective tumor suppressor. PMID: 27602961
These data reveal an essential role for the molecular chaperone GRP78 in IGF-IR signaling and implicate the use of GRP78 inhibitors in blocking IGF-IR signaling in hepatoma cells. PMID: 28389416
HMGA1 and MMP-11 may play a key role in the proliferation and progression of skin tumors in humans. PMID: 29374701
HMGA1 overexpression in adherent A2780 cells increased cancer stem cell properties, including proliferation, spheroid-forming efficiency and the expression of stemness-related genes. In addition, HMGA1 regulated ABCG2 promoter activity through HMGA1-binding sites. PMID: 27561949
Studies indicate that pseudogenes of the HMGA1 gene, that codes for the HMGA1a and HMGA1b proteins having a critical role in development and cancer progression. PMID: 26895108
study revealed a subset of potential development-associated miRNAs and suggests a novel regulatory axis for myogenesis in which miR-195/497 promote myogenic differentiation by repressing the HMGA1-Id3 pathway. PMID: 28255268
Findings demonstrate that a relationship exists between the HMGA1 rs146052672 variant and acute myocardial infarction, suggesting that defects at the HMGA1 locus may play a pathogenetic role. PMID: 27839822
SNP rs5498 in ICAM-1 gene and IVS5-13insC variant in HMGA1 gene were not associated with the susceptibility of DR in the Chinese T2DM cohort. PMID: 26717491
We show that the chromatin remodeling protein HMGA1 functions as a downstream effector of these biological responses to miR-296-5p and regulates Sox2 expression, a master driver of cell stemness, by modifying chromatin architecture at the Sox2 promoter PMID: 26898758
tissue microarray revealed that HMGA1 was expressed in thyroid carcinoma more than that in normal thyroid tissues ; expression of HMGA1 and MMP-2 was identified to be positively correlated . The present study established the first link between HMGA1 and TGF-b1 in the regulation of thyroid cancer proliferation and invasion PMID: 28393241
our research indicated an H19-miR138-HMGA1 pathway in regulating the migration and invasion of colon cancer, providing new insight for treatment of colon cancer. PMID: 28358427
The results reported here indicate a critical role of HMGA1 in the development of glioblastoma multiforme; its suppression impairs proliferation and self-renewal of brain tumor stem cells PMID: 27486901
miR-214 expression was poor while that of HMGA1 was high in cervical and colorectal cancer tissues. miR-214-re-expression or HMGA1 downregulation inhibited proliferation, migration and invasion of cancer cells while miR-214 inhibition had opposite effects. miR-214 was demonstrated to bind to the wild-type 3' untranslated region of HMGA1 but not with its mutant. PMID: 27537384
HMGA1 is frequently expressed in cirrhotic tissues and HCCs and its expression is associated with high Edmondson grade and worse prognosis in HCC. PMID: 27855356
Studies define a novel HMGA1-MMP-2 pathway involved in a subset of human carcinosarcomas and tumor progression in murine models. PMID: 27001612
HMGA1 may function as an oncogene and modulate endometrial cancer cell migration and invasion by activating Wnt/beta-catenin pathway. PMID: 27470368
Increased HMGA1 in pulmonary arterial endothelial cells resulting from dysfunctional BMPr2 signaling can transition endothelium to smooth muscle-like cells associated with pulmonary arterial hypertension. PMID: 27045138
in splenic hemangioma with t(3;6)(q26;p21), the entire coding region of HMGA1 comes under the control of the TBL1XR1 promoter, bringing about dysregulation of HMGA1 PMID: 26708416
This is the first report for the association between HMGA1 and S100A13 expression in the modulation of thyroid cancer growth and invasion. PMID: 27008379
Data indicate that the high mobility group A1 (HMGA1) proteomic signature has prognostic value in breast cancer. PMID: 26527623
In breast cancer patients, high levels of HMGA1 and CCNE2 expression are associated with the YAP/TAZ signature. PMID: 26265440
HMGA1 is overexpressed in human breast carcinomas and its levels are associated with a particular endocrine status. PMID: 26251519
Report epigenetic modification of miRNA targets HMGA transcripts on pituitary cells. PMID: 25557289
Our data indicates that measuring urine high mobility group AT-hook 1 may serve as a useful diagnostic tool for serous epithelial ovarian cancer. PMID: 25586095
The HMGA1 rs146052672 genotype variant had an overall statistically significant effect on increasing the risk of development of type 2 diabetes mellitus in Europeans. (Meta-analysis) PMID: 26296198
Somatic mutation of mtDNA was associated with better prognosis of head and neck squamous cell carcinoma. PMID: 24976238
Results show that HMGA1 binds CRMP1 promoter and regulates its expression in medulloblastoma cells. PMID: 26009886
HMGA1 plays an important role on breast cancer aggressiveness and prognosis and may act as a promising target for prognostic prediction. PMID: 25772486
CBX7/HMGA1b/NF-kappaB could take part in the same transcriptional mechanism that finally leads to the regulation of SPP1 gene expression in papillary thyroid carcinoma. PMID: 25595895
The results of the present study indicate that miR-26a may be associated with human breast carcinogenesis, which inhibits tumor cell proliferation by targeting HMGA1. PMID: 25755724
High expression of HMGA1 along with miR-26a downregulation indicated a worse prognosis for patients with urothelial bladder cancer. PMID: 25455159
High HMGA1 expression is associated with colorectal cancer. PMID: 24696416
Increased HMGA1 expression in human chondrocytes is associated with increased expression of IGFBP-3. PMID: 23036517
HMGA1 plays an important role on NSCLC progression and prognosis and may act as a convictive biomarker for prognostic prediction PMID: 25344216
results indicated that miR-142-3p may function as a tumor suppressor by targeting HMGA1 in osteosarcoma PMID: 24803022
The prognostic significance of the HMGA-1 antigen was not confirmed in pituitary adenomas with residual tumor growth. PMID: 24452592
our data indicate a critical role for HMGA1 in regulating both self-renewal and the symmetric/asymmetric division ratio in cancer stem cells PMID: 24833610
HMGA1 is a key molecular switch required by cancer cells for tumor progression and a poorly differentiated, stem-like state. The HMGA1 gene and proteins are expressed at high levels in all aggressive tumors studied to date. Review. PMID: 24684280
a differential control exerted by both Oct-1 and Oct-2 in the transcriptional regulation of the HMGA1 gene PMID: 24367622
HMGA1 gene variant may have a role in type 2 diabetes, body mass index, and high-density lipoprotein cholesterol in a Hispanic-American population PMID: 24148075
Results support a model in which the HMGA1/TAR interaction prevents the binding of transcription-activating cellular co-factors and Tat, subsequently leading to reduced HIV-1 transcription. PMID: 23392246

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Involvement in disease

A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51B.

Subcellular Location

Nucleus. Chromosome.

Protein Families

HMGA family

Database Links

HGNC: 5010

OMIM: 600701

KEGG: hsa:3159

STRING: 9606.ENSP00000308227

UniGene: Hs.518805

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Code	CSB-RA599206A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA599206A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA599206A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of A431 cell with CSB-RA599206A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing BxPC-3 cells stained with CSB-RA599206A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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HMGA1 Recombinant Monoclonal Antibody

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