HNRNPC Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA010605A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, JK whole cell lysate, Raji whole cell lysate, MCF7 whole cell lysate All lanes: HNRNPC antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34, 33, 36, 28 kDa Observed band size: 42 kDa IHC image of CSB-RA010605A0HU diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody IHC image of CSB-RA010605A0HU diluted at 1:300 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody IHC image of CSB-RA010605A0HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody Immunofluorescence staining of Hela cell with CSB-RA010605A0HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of HepG2 cell with CSB-RA010605A0HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing MCF7 cells stained with CSB-RA010605A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was Rabit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed. Immunoprecipitating HNRNPC in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-RA010605A0HU in Hela whole cell lysate. Lane 2:CSB-RA010605A0HU（3µg）+ Hela whole cell lysate（500µg） Lane 3: Hela whole cell lysate(20µg) For western blotting, Goat polyclonal to rabbit IgG antibody was used as the secondary antibody (1/50000)
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

P07910

Target Names

HNRNPC

Alternative Names

C1 antibody; C2 antibody; Heterogeneous nuclear ribonucleoprotein C (C1/C2) antibody; Heterogeneous nuclear ribonucleoprotein C antibody; Heterogeneous nuclear ribonucleoproteins C1/C2 antibody; HNRNP antibody; hnRNP C1 / hnRNP C2 antibody; hnRNP C1/C2 antibody; Hnrnpc antibody; HNRPC antibody; HNRPC_HUMAN antibody; MGC104306 antibody; MGC105117 antibody; MGC117353 antibody; MGC131677 antibody; Nuclear ribonucleoprotein particle C1 protein antibody; Nuclear ribonucleoprotein particle C2 protein antibody; SNRPC antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human HNRNPC

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9G1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:20-1:200
IP	1:200-1:1000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

HNRNPC, also known as heterogeneous nuclear ribonucleoproteins C1/C2, plays a central role in pre-mRNA processing and nuclear RNA metabolism. As a key component of the spliceosome complex, this protein influences alternative splicing decisions that can profoundly affect gene expression programs. Researchers investigating epigenetics, nuclear signaling, and RNA biology will find HNRNPC an essential target for understanding how post-transcriptional regulation shapes cellular phenotypes.

This recombinant monoclonal antibody, generated from clone 9G1 in rabbit host, offers the reproducibility that rigorous research demands. Because recombinant technology produces antibodies from defined sequences rather than animal immunizations, you can expect consistent performance across experiments and over time, eliminating the lot-to-lot variability that can complicate longitudinal studies or multi-site collaborations.

Validation data demonstrates robust performance across diverse experimental platforms. Western blot analysis detects HNRNPC in multiple human cell lines including HeLa, 293, Jurkat, Raji, and MCF7, with an observed band at approximately 42 kDa. This migration above the predicted molecular weights of 28–36 kDa likely reflects post-translational modifications such as phosphorylation or other regulatory modifications common to hnRNP family members. Immunohistochemistry staining has been validated in paraffin-embedded human brain, breast cancer, and kidney tissues, providing options for studying HNRNPC expression across normal and pathological contexts. Immunofluorescence applications confirm nuclear localization patterns in HeLa and HepG2 cells, while flow cytometry and immunoprecipitation validations in MCF7 and HeLa cells respectively expand your methodological flexibility.

Whether you are mapping splicing factor networks, investigating nuclear organization, or exploring HNRNPC dysregulation in disease models, this antibody provides a reliable tool for your research workflow.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules. Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. N6-methyladenosine (m6A) has been shown to alter the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch', facilitating binding of HNRNPC, leading to regulation of mRNA splicing.

Gene References into Functions

APP levels then decrease progressively as a function of age in close relationship with the gradual normalization of FMRP and hnRNP C levels. PMID: 26048669
Endoplasmic reticulum resident chaperone GRP78, mitochondrial protein Prohibitin and heterogeneous nuclear ribonucleoprotein hnRNPC (C1/C2) have been shown to interact with viral RNA. Hence it is proposed that these are the principle candidates governing endoplasmic reticulum stress-induced apoptosis in JEV infection. PMID: 28102850
Our results indicate that a balance between lncRNA SNHG1 and hnRNPC regulates p53 activity and p53-dependent apoptosis upon doxorubicin treatment, and further indicate that a change in lncRNA subcellular localization under specific circumstances is biologically significant. PMID: 28264987
These data suggest an interplay between CELF2 and hnRNP C as the mechanistic basis for activation-dependent alternative splicing of TRAF3 exon 8. PMID: 28031331
These data indicate that hnRNPC1/C2 binds to both DNA and RNA and influences both gene expression and RNA splicing, but these actions do not appear to be linked through 1,25(OH)2D-mediated induction of transcription. PMID: 27672039
These findings suggest that hnRNP C1/C2 is involved in dengue virus replication at the stage of viral RNA synthesis. PMID: 25890165
HnRNP C, YB-1 and hnRNP L coordinately enhance skipping of human MUSK exon 10 to generate a Wnt-insensitive MuSK isoform. PMID: 25354590
Our results suggested that hnRNP C1 controls HPV16 late gene expression. PMID: 25878250
m(6)A-switch-regulated HNRNPC-binding activities affect the abundance as well as alternative splicing of target mRNAs, demonstrating the regulatory role of m(6)A-switches on gene expression and RNA maturation PMID: 25719671
PTBP1 and hnRNP C repress exon 3 inclusion, and that downregulation of PTBP1 inhibited BIM-mediated apoptosis. PMID: 24743263
down-regulation of MALAT-1 expression compromised the cytoplasmic translocation of hnRNP C in the G2/M phase and resulted in G2/M arrest PMID: 23973260
Heterogeneous nuclear ribonucleoprotein C is a key regulator of BRCA gene expression and homologous recombination-based DNA repair. PMID: 23585894
By preventing U2AF65 binding to Alu elements, hnRNP C plays a critical role as a genome-wide sentinel protecting the transcriptome. PMID: 23374342
The data indicated that hnRNPC controls the aggressiveness of glioblastoma cells through the regulation of PDCD4. Silencing of hnRNPC lowered miR-21 levels, in turn increasing the expression of PDCD4, suppressing Akt and p70S6K activation. PMID: 22907752
p27kip1 upregulated by hnRNPC1/2 antagonizes CagA-mediated pathogenesis. PMID: 22404445
study shows mechanism by which Pol II transcripts are classified according to length; heterotetramer of hnRNP C1/C2 measures the length of the transcripts like a molecular ruler, by binding to unstructured RNA regions longer than 200 to 300 nucleotides PMID: 22461616
One of the identified proteins, hnRNPC, was found to interact with small hepatitis delta virus antigen in vitro and in vivo in human liver cells. PMID: 21774814
Studies indicated that DDX21, HNRNPC, and RCC2 were isolated from Ku86 multicomponent complex in response to DNA damage. PMID: 20873769
HNRNP C recognizes uridine tracts with a defined long-range spacing consistent with HNRNP particle organization. PMID: 20601959
FMRP represses translation by recruiting APP mRNA to processing bodies, whereas hnRNP C promotes APP translation by displacing FMRP, thereby relieving the translational block. PMID: 20473314
The authors report here that the 5' end of poliovirus negative-strand RNA is capable of interacting with endogenous hnRNP C, as well as with poliovirus nonstructural proteins. PMID: 20164237
The authors propose that hnRNP C interacts with poliovirus RNA and replication proteins to increase the efficiency of viral genomic RNA synthesis. PMID: 20189623
phosphorylation of heterogeneous nuclear ribonucleoprotein C1/C2 in response to physiologic levels of hydrogen peroxide in endothelial cells PMID: 11877401
nuclear ribonucleoproteins C1 and C2 are part of the RNP complex that forms on XIAP IRES, the cellular levels of hnRNPC1 and -C2 parallel the activity of XIAP IRES and the overexpression of hnRNPC1-C2 specifically enhanced translation of XIAP IRES PMID: 12482981
hnRNP C, via internal ribosomal entry site binding, modulates translation of c-myc mRNA in a cell cycle phase-dependent manner PMID: 12509468
the acidic C-terminal domain of hnRNP-C1/C2 could be a regulatory domain and may play an important role in the regulation of mRNA binding by hnRNP-C1/C2. PMID: 12564933
a novel nuclear export is activated by the ROCK signaling pathway to exclude hnRNP C1/C2 from nucleus, by which the compartmentalization of specific hnRNP components is disturbed in apoptotic cells PMID: 15494373
CK1alpha-mediated phosphorylation modulates the mRNA binding ability of hnRNP-C PMID: 15687492
findings suggest that hnRNP C1 has a role in positive-strand RNA synthesis in poliovirus-infected cells, possibly at the level of initiation PMID: 15731220
upstream element in human papillomavirus type 16 interacted specifically with CstF-64, hnRNP C1/C2 & polypyrimidine tract binding protein, suggesting these factors were enhancing or regulating polyadenylation at the HPV-16 early polyadenylation signal PMID: 15767428
Direct in vivo interaction of hnRNPC with the urokinase receptor mRNA 3'UTR was demonstrated. PMID: 16010978
Results describe the purification of a locus control region-associated remodeling complex consisting of heterogeneous nuclear ribonucleoprotein C1/C2, SWI/SNF, and MeCP1 as a single homogeneous complex. PMID: 16217013
These results demonstrate that hnRNP C1/C2 is involved in maintenance of cellular homeostasis besides cellular differentiation and proliferation. PMID: 16960656
in addition to its RNA-processing functions, hnRNP C1/C2 may be a key determinant of the temporal patterns of VDRE occupancy PMID: 17071612
regulation of IRES-mediated translation by hnRNP C1/C2 and Unr might be important in mitosis PMID: 17159903
A novel cis-element in the 5' coding region of p53 mRNA and its interaction with heterogeneous nuclear ribonucleoprotein (hnRNP)C1/C2, is described. PMID: 18296503
Intracellular NS1 protein of dengue virus interacts with hnRNP C1/C2. PMID: 18471994
The RNA binding domain of heterogeneous nuclear ribonuclear protein C binds and stabilizes urokinase-type plasminogen activator receptor (uPAR) mRNA, attributing the key role of this domain in hnRNPC-mediated uPAR regulation in lung epithelial cells. PMID: 18494499
The H(2)O(2) responsive pre-mRNA binding protein hnRNP-C is up-regulated in atherosclerosis. PMID: 18508286
anti-hnRNP C1/C2 antibody hampered splicing of SMN1 exon 7, but did not affect splicing of SMN2 exon 7 PMID: 19628962
endogenous hnRNP C and PTEN interact and co-localize within the nucleus PMID: 19740742
This protein has been found differentially expressed in the Wernicke's Area from patients with schizophrenia. PMID: 19405953

Hide All

Subcellular Location

Nucleus. Note=Component of ribonucleosomes.

Protein Families

RRM HNRPC family, RALY subfamily

Database Links

HGNC: 5035

OMIM: 164020

KEGG: hsa:3183

STRING: 9606.ENSP00000319690

UniGene: Hs.508848

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address