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CUSABIO administered a human HNRNPC-derived peptide to an animal to elicit an immune response. B cells were subsequently isolated from the immunized animal and fused with myeloma cells, generating hybridoma cells. Through screening, a single hybridoma cell clone that produces the desired HNRNPC-specific antibody was identified and selected. RNA was extracted from the chosen hybridoma cells, and the variable regions of the HNRNPC antibody's heavy and light chains were isolated and amplified using reverse transcription PCR. These amplified HNRNPC antibody variable regions were then cloned into an expression vector and transfected into a host cell line for expression purposes. The resulting HNRNPC recombinant monoclonal antibodies were purified from the cell culture supernatant using affinity chromatography. Six applications including ELISA, WB, IHC, IF, FC, and IP were employed to confirm the binding specificity and affinity of this antibody. Notably, this antibody exhibits recognition specifically for human HNRNPC protein.
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