Product Details

Uniprot No.

P61978

Target Names

HNRNPK

Alternative Names

CSBP antibody; dC stretch binding protein antibody; FLJ41122 antibody; Heterogeneous nuclear ribonucleoprotein K antibody; hnRNP K antibody; HNRNPK antibody; HNRPK antibody; HNRPK_HUMAN antibody; Transformation up regulated nuclear protein antibody; Transformation up-regulated nuclear protein antibody; Transformation upregulated nuclear protein antibody; TUNP antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human HNRNPK

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

3G7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:20-1:200
IP	1:200-1:1000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Heterogeneous nuclear ribonucleoprotein K (HNRNPK) serves as a critical regulator of gene expression, functioning at multiple levels from transcription to translation. This multifunctional RNA-binding protein participates in chromatin remodeling, mRNA processing, and signal transduction pathways, making it a key player in cellular responses to stress and proliferation signals. Also known as transformation up-regulated nuclear protein (TUNP), HNRNPK has garnered significant attention in cancer research due to its altered expression patterns in various malignancies.

This recombinant monoclonal antibody, clone 3G7, offers the consistency and reproducibility that demanding research protocols require. Generated against a synthetic peptide derived from human HNRNPK and produced using recombinant technology in rabbit host, this antibody provides sequence-defined specificity with minimal lot-to-lot variation, ensuring your experimental results remain comparable across studies.

Validation data demonstrates robust performance across multiple applications. Western blot analysis confirms reliable detection in diverse human cell lines including HeLa, MCF-7, HepG2, 293T, 293, U87, and SH-SY5Y lysates. The observed band at approximately 60 kDa runs slightly higher than the predicted molecular weight of 49-52 kDa, a shift commonly attributed to post-translational modifications such as phosphorylation or methylation that HNRNPK is known to undergo. Immunohistochemistry validation in human pancreatic and cervical cancer tissues demonstrates clear nuclear staining patterns, while immunofluorescence studies in A549 cells reveal expected nuclear localization. Immunoprecipitation from HepG2 lysates further confirms the antibody's utility for protein interaction studies.

For researchers investigating epigenetics, nuclear signaling, or RNA biology, this antibody provides a versatile tool adaptable to multiple experimental workflows.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction. As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest.

Gene References into Functions

Data show that heterogeneous nuclear ribonucleoprotein K (hnRNP K) and influenza virus NS1A binding protein (NS1-BP) regulate host splicing events and that viral infection causes mis-splicing of some of these transcripts. PMID: 29921878
Study findings underscore the biological significance of MRPL33-L and hnRNPK in the tumor formation and identifies hnRNPK as a critical splicing regulator of MRPL33 pre-mRNA in cancer cells. PMID: 28869607
hnRNPK regulates PLK1 expression by competing with the PLK1-targeting miRNAs, miR-149-3p and miR-193b-5p. PMID: 28708135
we discovered that hnRNPK plays an important role in bladder cancer, suggesting that it is a potential prognostic marker and a promising target for treating bladder cancer. PMID: 27862976
The hnRNPK positively regulates the level of prostate tumor overexpressed 1-antisense 1 which harbors five binding sites for miR-1207-5p. The knockdown of hnRNPK or PTOV1-AS1 increased the enrichment of heme oxygenase-1 mRNA in miR-1207-5p-mediated miRNA-induced silencing complex and thus suppressed the expression of heme oxygenase-1. PMID: 28228215
High HNRNPK expression is associated with pancreatic cancer. PMID: 28423622
Down-regulation of DAB2IP correlated negatively with hnRNPK and MMP2 expressions in CRC tissues. In conclusion, our study elucidates a novel mechanism of the DAB2IP/hnRNPK/MMP2 axis in the regulation of CRC invasion and metastasis, which may be a potential therapeutic target. PMID: 28335083
Tumor cells bearing a p53 mutation showed increased damage levels and delayed repair. Knockdown of hnRNPK applied simultaneously with irradiation reduced colony-forming ability and survival of tumor cells. Taken together, our data shows that hnRNPK is a relevant modifier of DNA damage repair and tumor cell survival. We therefore recommend further studies to evaluate the potential of hnRNPK as a drug target for improvement PMID: 28426877
Data suggest that hnRNPK plays role in heat shock response of cells by regulating HSF1; hnRNPK inhibits HSF1 activity, resulting in reduced expression of HSP27 and HSP70 mRNAs; hnRNPK also down-regulates binding of HSF1 to heat shock response element. (hnRNPK = heterogeneous-nuclear ribonucleoprotein K; HSF1 = heat shock transcription factor 1; HSP = heat-shock protein) PMID: 28592492
KRAS-mutant colorectal carcinoma shows intrinsic radioresistance along with rapid upregulation of hnRNP K in response to ionizing radiation that can effectively be targeted by MEK inhibition. PMID: 27793696
Nujiangexathone A, a novel compound from Garcinia nujiangensis, down-regulates hnRNPK levels in cervical tumor cells, inducing cell cycle arrest. PMID: 27424288
Our study also suggests that loss of function variants in HNRNPK should be considered as a molecular basis for patients with Kabuki-like syndrome PMID: 26954065
these findings support a critical role for hnRNP K in the regulation of autophagy in drug-resistant leukemia cells, and this RNP is therefore a potential target of clinical drug-resistance treatment. PMID: 27155326
hnRNP K is a multifunctional protein that can regulate both oncogenic and tumor suppressive pathways through a plethora of chromatin-, DNA-, RNA-, and protein-mediated activates, suggesting its aberrant expression may have broad-reaching cellular impacts. (Review) PMID: 27049467
These results establish the role of hnRNP K and PCPB1 in the translational control of morphine-induced MOR expression in human neuroblastoma (NMB) cells as well as cells stably expressing MOR (NMB1). PMID: 27292014
HnRNP K is a promising tissue biomarker for diagnosing gastric cancer. PMID: 27278897
CASC11 can target heterogeneous ribonucleoprotein K (hnRNP-K) to activate WNT/beta-catenin signaling in colorectal cancer cells to promote tumor growth and metastasis. PMID: 27012187
Data show that heterogeneous nuclear ribonucleoprotein K (hnRNPK) stabilized of cellular FLICE-inhibitory protein (c-FLIP) protein through inhibition of glycogen synthase kinase 3 beta (GSK3beta) Ser9 phosphorylation during the TNF-related aoptosis-inducing ligand (TRAIL)-induced apoptosis. PMID: 26972480
HhnRNP-K-mediated regulation of NMHC IIA mRNA translation contributes to the control of enucleation in erythropoiesis. PMID: 26823606
hnRNP K binds miR-122, a mature liver-specific microRNA required for Hepatitis C virus replication. PMID: 26330540
The authors identified two new human proteins that interact with Ehrlichia chaffeensis EtpE-C: CD147 and heterogeneous nuclear ribonucleoprotein K (hnRNP-K). PMID: 26530384
Data show that proto-oncogene protein c-myc is upregulated by sumoylated heterogeneous nuclear ribonucleoprotein K (hnRNP K) at the translational level in Burkitt's lymphoma cells. PMID: 26317903
hnRNP K may be a key molecule involved in cell motility in RCC cells PMID: 26713736
It describes the identification of heterogeneous nuclear ribonucleoprotein K (hnRNPK) as one of the composite element binding factors(CEBF) that acts as transactivator of PXR promoter. PMID: 26586566
RTVP-1 regulates glioma cell spreading, migration and invasion and that these effects are mediated via interaction with N-WASP and by interfering with the inhibitory effect of hnRNPK on the function of this protein. PMID: 26305187
we investigate the role of hnRNP K in the radioresistance of malignant melanoma cells PMID: 26136337
These results indicate that dengue virus type 2 and Junin virus induce hnRNP K cytoplasmic translocation to favor viral multiplication. PMID: 25865411
Data implicate hnRNPK in the development of hematological disorders and suggest hnRNPK acts as a tumor suppressor. PMID: 26412324
Inhibition of CDK2 phosphorylation blocked phosphorylation of hnRNP K, preventing its incorporation into stress granules (SGs). Due to interaction between hnRNP K with TDP-43, the loss of hnRNP K from SGs prevented accumulation of TDP-43. PMID: 25410660
HNRNPK might determine efficiency of Hepatitis C virus particle production by limiting the availability of viral RNA for incorporation into virions. PMID: 25569684
Data indicate that twenty proteins were identified as binding partners of the primary activating element in the heterogeneous nuclear ribonucleoprotein K (hnRNP K) promoter. PMID: 25497182
hnRNP K interacts with EV71 5' UTR, which is required for efficient synthesis of viral RNA.[review] PMID: 26164948
hnRNPK is potentially implicated in the radiogenic response of HNSCC. PMID: 25281771
hnRNP K plays an important role in the mitotic process in colon cancer cells.hnRNP K upregulates NUF2 and promotes the tumorigenicity of colon cancer cells. PMID: 25701787
These findings functionally integrate K17, hnRNP K, and gene expression along with RSK and CXCR3 signaling in a keratinocyte-autonomous axis and provide a potential basis for their implication in tumorigenesis PMID: 25713416
HnRNP K can induce MMP12 expression and enzyme activity through activating MMP12 promoter, which promotes cell migration and invasion in nasopharyngeal carcinoma cells. PMID: 24885469
Results indicate that the interaction between the AR and hnRNP K has an important role in the progression of prostate cancer. PMID: 24626777
hnRNP K and PU.1 act synergistically during granulocytic differentiation, hnRNP K seems to have a negative effect on PU.1 activity during monocytic maturation PMID: 25005557
CNBP overexpression caused increase of cell death and suppression of cell metastasis through its induction of G-quadruplex formation in the promoter of hnRNP K resulting in hnRNP K down-regulation PMID: 24594223
Data show that SET accumulation up-regulated hnRNPK mRNA and total/phosphorylated protein, promoted hnRNPK nuclear location, and reduced Bcl-x mRNA levels. PMID: 24508256
NS1-BP-hnRNPK complex is a key mediator of influenza A virus gene expression. PMID: 23825951
These studies demonstrate hnRNP K to be a multifunctional protein that supports vesicular stomatitis virus infection via its role(s) in suppressing apoptosis of infected cells. PMID: 23843646
hnRNPK may play a role in recruitment of XRN2 to gene loci thus regulating coupling 3'-end pre-mRNA processing to transcription termination. PMID: 23857582
heterogeneous nuclear ribonucleoprotein K (hnRNPK), a protein known to integrate multiple signal transduction pathways with gene expression, as a SERT distal polyadenylation element binding protein PMID: 23798440
Prolonged downregulation of hnRNP K using small interfering RNA significantly decreased cell viability and increased apoptosis in HCC cell lines in a p53-independent manner. PMID: 23455382
Caspase-3 cleaves hnRNP K in erythroid differentiation. PMID: 23519117
HnRNP K controlled expression of IE2 protein of Human cytomegalovirus during viral replication. PMID: 23099853
hnRNP-K regulates extracellular matrix, cell motility, and angiogenesis pathways. Involvement of the selected genes (Cck, Mmp-3, Ptgs2, and Ctgf) and pathways was validated by gene-specific expression analysis PMID: 23564449
ATM-dependent phosphorylation of heterogeneous nuclear ribonucleoprotein K promotes p53 transcriptional activation in response to DNA damage. PMID: 23343766
SUMO modification plays a crucial role in the control of hnRNP-K's function as a p53 co-activator in response to DNA damage by UV PMID: 23092970

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Involvement in disease

Au-Kline syndrome (AUKS)

Subcellular Location

Cytoplasm. Nucleus, nucleoplasm. Cell projection, podosome. Note=Recruited to p53/TP53-responsive promoters, in the presence of functional p53/TP53 (PubMed:16360036). In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear (PubMed:18775702).

Database Links

HGNC: 5044

OMIM: 600712

KEGG: hsa:3190

STRING: 9606.ENSP00000365439

UniGene: Hs.522257

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Code	CSB-RA010611A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate, 293 whole cell lysate, U87 whole cell lysate, SH-SY5Y whole cell lysate All lanes: HNRNPK antibody at 1.3μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 51, 52, 49 KDa Observed band size: 60 KDa IHC image of CSB-RA010611A0HU diluted at 1:130.5 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-RA010611A0HU diluted at 1:130.5 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of A549 cells with CSB-RA010611A0HU at 1:43.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). Immunoprecipitating HNRNPK in HepG2 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-RA010611A0HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA010611A0HU (3μg) + HepG2 whole cell lysate (500μg) Lane 3: HepG2 whole cell lysate (20μg)
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HNRNPK Recombinant Monoclonal Antibody

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