Product Details

Uniprot No.

Target Names

Alternative Names

ARMD7 antibody; CARASIL antibody; High-temperature requirement A serine peptidase 1 antibody; HtrA antibody; HtrA serine peptidase 1 antibody; HTRA1 antibody; HTRA1_HUMAN antibody; IGFBP5 protease antibody; L56 antibody; ORF480 antibody; Protease serine 11 (IGF binding) antibody; protease serine 11 antibody; PRSS11 antibody; Serine protease 11 antibody; Serine protease HTRA1 antibody; Serine protease HTRA1 precursor antibody

Species Reactivity

Human

Immunogen

Recombinant Human HTRA1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

29C11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

HTRA1 is a secreted serine protease with significant roles in extracellular matrix remodeling, TGF-β signaling regulation, and cellular stress responses. This multifunctional enzyme has garnered considerable research attention due to its associations with age-related macular degeneration and cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy, making it a compelling target for investigators studying neurodegenerative conditions, vascular biology, and ocular disease pathways.

This recombinant monoclonal antibody, clone 29C11, offers the reproducibility and consistency that demanding research protocols require. Generated against recombinant human HTRA1 protein and produced using recombinant technology, this antibody provides sequence-defined specificity with minimal lot-to-lot variation, ensuring that your experimental conditions remain stable across extended studies or multi-site collaborations. The human IgG1 isotype format and affinity chromatography purification contribute to reliable performance in validated applications.

Validation testing has confirmed this antibody's utility in immunohistochemistry applications using human tissue samples. Staining performed on paraffin-embedded human placenta tissue at 1:200 dilution demonstrated clear target detection following citrate buffer antigen retrieval, with visualization achieved through HRP-polymer secondary detection and DAB chromogen development. The antibody is also suitable for ELISA-based detection, with recommended IHC dilutions ranging from 1:50 to 1:200 to accommodate optimization across different tissue types and experimental conditions.

For researchers investigating signal transduction pathways, particularly those involving IGF-binding protein processing or TGF-β family regulation, this antibody provides a dependable tool for characterizing HTRA1 expression and localization in human samples. The liquid format with glycerol-based storage buffer supports long-term stability when maintained at recommended temperatures.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets.

Gene References into Functions

Nuclear downregulation of HtrA1 is associated with a better prognosis in women with high grade serous ovarian carcinoma. PMID: 30131069
Case Report: Novel compound heterozygous mutations in HTRA1 causing CARASIL in Chinese patient. PMID: 30068478
regulates odontoblastic differentiation of dental pulp cells through activation of the TGF-beta1/Smad signaling pathway PMID: 29580722
the aberrant expression of HTRA1 or HTRA4 may be involved in the onset of preeclampsia, and increased HTRA1 or HTRA4 expression may affect trophoblast functions. PMID: 30015931
HtrA1 contributes to the development of keloid lesions as matrix protease by remodelling keloid-specific ECM or cell surface molecules. PMID: 29695130
Studies indicate a significantly different high-temperature requirement factor A1 (HtrA1) expression in cancer and non-cancer tissue [Meta-analysis]. PMID: 29409460
HtrA1 overexpression further leads to impaired apical processes and decreased phagocytosis, an essential function for photoreceptor survival. PMID: 29269042
Results suggest that HTRA1 is involved in the pathogenesis of scars through regulating activation of latent TGF-beta1 in keloid fibroblasts. PMID: 29412803
the CADASIL-like family disease may be caused by heterozygous HTRA1 gene mutation, which leads to autosomal dominant hereditary cerebral small vessel disease. PMID: 29561953
Case Report/Review: novel missense mutation in HTRA1 associated with phenotype of CARASIL. PMID: 28628911
The rs11200638-rs2672598 joint genotype AA-CC conferred higher risk to exudative exudative age-related macular degeneration (AMD) than polypoidal choroidal vasculopathy (PCV). PMID: 27338780
a possible proteolytic processing mechanism of mutant TGFBIp by HTRA1 and peptides generated by mutant protein may form the beta-amyloid core of corneal aggregates in Corneal dystrophic patients. PMID: 28689406
HtrA1 Proteolysis of ApoE In Vitro Is Allele Selective PMID: 27379525
The observation of this study further supports the pathogenic role of the heterozygous HTRA1 mutations in familial cerebral small vessel disease. PMID: 28782182
These findings suggest that the variation in the risk for age-related macular degeneration associated with chromosome 10q26 is likely due to variation in HTRA1 expression. PMID: 28659708
HtrA1 role in the cisplatin resistance in colon cancer PMID: 28667026
HtrA1 could serve as a marker to identify ulcerative colitis of >10 year duration patients at high risk of developing colorectal cancer. PMID: 28586045
To our knowledge, this is the first time the association between rs11200638 and overall age-related macular degeneration has been reported in South America. PMID: 28846052
because the ARMS2/HTRA1 genes are positioned at a locus on chromosome 10q26 in a region with strong linkage disequilibrium, it is difficult to distinguish the functions of the individual genes. a review of recent epidemiological studies of Age-related macular degeneration(AMD) is offered. An argument for a definite correlation between the ARMS2 gene and AMD is presented PMID: 28583181
The expression of HtrA1 was strongly related to the T2 value, suggesting that HtrA1 plays an important role in the pathological process of intervertebral disc degeneration. PMID: 28432852
These results suggest that the initiation stage of polypoidal choroidal vasculopathy is mediated by proteolytic degradation of extracellular matrix proteins attributable to increased HTRA1 activity PMID: 28941979
The findings of the present study provide evidence that CFH gene variants and ARMS2/HTRA1 genes play a major role in the genetic susceptibility to AMD in a Greek population. These findings are of direct relevance for disease and help mapping the genetic chart of AMD. PMID: 26848857
The function of the binding between MIF and HTRA1 is to inhibit the proteolytic activity of HTRA1. PMID: 28726057
Results show that HTRA1 is epigenetically silenced in HCT116 colon carcinoma cells and during early stages of tumorigenesis in a mouse model of intestinal cancer. Downregulation of HTRA1 causes a multiple phenotypes that are hallmarks of cancer cells including increased proliferation of mouse embryonic fibroblasts, as well as chromosome and centrosome amplifications. PMID: 27388476
High HTRA1 expression is associated with cervical cell proliferation. PMID: 27809811
Data indicate HtrA serine peptidase 1 (HTRA1) involvement in Age-related macular degeneration (AMD) pathogenesis. PMID: 27841854
Variants in HTRA1 are not associated with age-related macular degeneration. PMID: 27879347
Results show the heterozygous missense mutations p.G283E, p.P285L, p.R302Q, and p.T319I in the HTRA1 gene in 8 patients with symptomatic cerebral small vessel disease; mutant HTRA1s observed in manifesting heterozygotes might result in an impaired HTRA1 activation cascade of HTRA1 or be unable to form stable trimers PMID: 27164673
FN and HtrA1 are localized in the placental key growth zones suggesting a pivotal role in maintaining the balance among the molecules involved in the placental development and differentiation PMID: 28076935
correlation between TGFb1 and pSmad2 as well as between HtrA1 and TGFb1 and the very significant increase of Ki67 in stromal compartment of eutopic endometrium suggest a possible involvement of HtrA1 in the pathogenesis of endometriosis. PMID: 26708185
Two synonymous polymorphisms in exon 1 of the HTRA1 gene result in a protein with altered thermophoretic properties. PMID: 26310622
significant increase of serum HtrA1 in early-onset pre-eclampsia PMID: 26187609
a frameshift mutation in the HTRA1 gene detected in a CARASIL pedigree resulted in reduced HTRA1 protein and increased TGF-beta1 expression, which may cause severe CARASIL and peripheral small arterial disease PMID: 25772074
Human HTRA1 expression is enhanced by Age-related macular degeneration specific indel mutation in the promoter region of HTRA1 gene, and this enhanced HTRA1 may be concerned with induce retinal neovasucularization. PMID: 27125063
Results does not conclusively support HTRA1 role as a tumor suppressor but suggest its possible prognostic role in many human tumors. PMID: 26035313
Studies indicate that the high-risk allele of the 10q26 locus encompasses three genes, PLEKHA1, ARMS2, and HTRA1 with high linkage disequilibrium. PMID: 26427389
Low HtrA1 expression is associated with gastric cancer. PMID: 25761858
HtrA1 expression was closely related to EMT, which might be a potential mechanism underlying metastasis of HCC PMID: 26403966
Low HtrA1 expression is significantly related to breast cancer poor prognosis parameters, and HtrA1 loss in sentinel nodes is related to metastasis of non sentinel nodes. PMID: 25530301
It was concluded that high expression of HtrA1 could significantly reverse multidrug resistance of hepatoma cells by targeting XIAP. PMID: 25776486
The growth of choroidal neovascularization in AMD would be affected by 2 genes: MMP20, a newly confirmed gene expressed in the retina, and ARMS2/HTRA1, a well-known susceptibility gene for AMD. PMID: 26337002
HtrA1 appeared as an immunohistochemical marker to predict behaviour of the meningioma, mainly the recurrence PMID: 25687108
HtrA1 plays a positive role in human periodontal ligament cells osteogenic differentiation and may regulate this process by TGFB1. PMID: 25726184
In this study, we found that the interaction of ARMS2 and ARMS2/HTRA1 is significantly associated with nAMD, and the interaction of CFH and ARMS2 is pronounced in PCV development in Chinese population. PMID: 25771815
HtrA1 expression in plasma cells could be correlated with the destruction of pathological periodontal tissue. PMID: 24979214
Central but not peripheral drusen location was strongly associated with both [CFH HTRA1] and [CFH HTRA1]. Only [CFH HTRA1] was significantly associated with increased central drusen area. PMID: 25627090
heterozygous HTRA1 mutations are an important cause of familial small vessel disease PMID: 26063658
Silence of the HTRA1 gene was associated with significantly higher levels of TGF-beta1, BMP4, and BMP2 mRNA and reduction in the proliferation and migration of ARPE-19 cells. PMID: 25550099
Gene variants in CFH, ARMS2 and HTRA1 are related to an increased risk of age-related macular degeneration in a northern Chinese population. PMID: 24865190
alpha-1-antitrypsin as substrate of HTRA1 synthetic PMID: 25329061

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Involvement in disease

Macular degeneration, age-related, 7 (ARMD7); Cerebral arteriopathy, autosomal recessive, with subcortical infarcts and leukoencephalopathy (CARASIL); Cerebral arteriopathy, autosomal dominant, with subcortical infarcts and leukoencephalopathy, 2 (CADASIL2)

Subcellular Location

Cell membrane. Secreted. Cytoplasm, cytosol.

Protein Families

Peptidase S1C family

Tissue Specificity

Widely expressed, with strongest expression in placenta (at protein level). Secreted by synovial fibroblasts. Up-regulated in osteoarthritis and rheumatoid arthritis synovial fluids and cartilage as compared with non-arthritic (at protein level).

Database Links

HGNC: 9476

OMIM: 600142

KEGG: hsa:5654

STRING: 9606.ENSP00000357980

UniGene: Hs.501280

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Code	CSB-RA835695MA1HU
Size	US$210
	Order now
Image	IHC image of CSB-RA835695MA1HU diluted at 1:200 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
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HTRA1 Recombinant Monoclonal Antibody

Product Details

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Customer Reviews and Q&A

Target Background