IL12&IL23 Recombinant Monoclonal Antibody

CLMF antibody; CLMF p35 antibody; CLMF p40 antibody; CLMF1 antibody; CLMF2 antibody; Cytotoxic lymphocyte maturation factor 1, p35 antibody; Cytotoxic lymphocyte maturation factor 2 antibody; Cytotoxic lymphocyte maturation factor 35 kDa subunit antibody; Cytotoxic lymphocyte maturation factor 40 kDa subunit antibody; IL 12A antibody; IL 2 subunit p40 antibody; IL-12 subunit p35 antibody; IL-12A antibody; IL-12B antibody; IL12 antibody; IL12, p35 subunit antibody; IL12, subunit p40 antibody; IL12A antibody; IL12A_HUMAN antibody; IL12B antibody; IL35 subunit antibody; Interleukin 12 alpha chain antibody; Interleukin 12 beta chain antibody; Interleukin 12 subunit beta antibody; Interleukin 12, p40 antibody; Interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) antibody; Interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) antibody; Interleukin 12B antibody; Interleukin-12 subunit alpha antibody; Natural killer cell stimulatory factor 1, 35 kD subunit antibody; natural killer cell stimulatory factor 2 antibody; Natural killer cell stimulatory factor, 40 kD subunit antibody; NF cell stimulatory factor chain 1 antibody; NFSK antibody; NK cell stimulatory factor chain 1 antibody; NK cell stimulatory factor chain 2 antibody; NKSF antibody; NKSF1 antibody; NKSF2 antibody; P35 antibody;CLMF antibody; CLMF p40 antibody; CLMF2 antibody; Cytotoxic lymphocyte maturation factor 40 kDa subunit antibody; IL 12B antibody; IL-12 subunit p40 antibody; IL-12B antibody; IL12 p40 antibody; IL12B antibody; IL12B_HUMAN antibody; IMD28 antibody; IMD29 antibody; Interleukin 12 beta chain antibody; Interleukin 12 p40 antibody; Interleukin-12 subunit beta antibody; NK cell stimulatory factor chain 2 antibody; NKSF antibody; NKSF2 antibody

Interleukin-12 and Interleukin-23 are heterodimeric cytokines that share the p40 subunit (IL-12B), making them central players in immune regulation and inflammatory responses. IL-12, composed of p35 and p40 subunits, drives Th1 differentiation and IFN-γ production, while IL-23 promotes Th17 cell maintenance and is implicated in autoimmune pathologies. The shared p40 subunit makes these cytokines attractive therapeutic targets and essential subjects in immunology research exploring infection, cancer immunity, and inflammatory disease mechanisms.

This recombinant monoclonal antibody, clone 14D4, offers researchers the reproducibility and consistency that recombinant technology provides. Unlike traditional hybridoma-derived antibodies, this sequence-defined reagent ensures lot-to-lot uniformity, eliminating variability that can compromise longitudinal studies or multi-site collaborations. The human IgG1 isotype format and affinity-chromatography purification deliver a high-quality reagent suitable for demanding experimental workflows.

Validation data demonstrates robust performance across multiple applications. Functional ELISA testing confirms strong binding activity, with immobilized human IL-12B and IL-12A protein showing an EC50 of 1.042–1.545 ng/mL, indicating excellent sensitivity for detection assays. For tissue-based studies, immunohistochemistry validation in paraffin-embedded human lymph node tissue at 1:50–1:200 dilutions reveals clear target localization, with optimized antigen retrieval using citrate buffer at pH 6.0 on the Leica Bond system.

The antibody is supplied in a glycerol-based PBS buffer with Proclin 300 preservative, ensuring stability during storage at -20°C or -80°C. Researchers investigating cytokine biology, immune cell differentiation, inflammatory disorders, or therapeutic antibody development will find this reagent particularly valuable for characterizing IL-12/IL-23 pathway components in human samples.