IL12&IL23 monoclonal antibody

Datasheet

Code	CSB-RA011587MA1HU
Size	US$210
	Order now
Image	The Binding Activity of IL12B&IL12A with Anti-IL12/IL23 monoclonal antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human IL12B&IL12A (CSB-MP4155HU) at 1 μg/mL can bind Anti-IL12/IL23 recombinant antibody, the EC₅₀ is 1.042-1.545 ng/mL. IHC image of CSB-RA011587MA1HU diluted at 1:50 and staining in paraffin-embedded human lymphnode tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Customer Reviews and Q&A

Product Details

Uniprot No.

P29459&P29460

Target Names

IL12&IL23

Alternative Names

IL12&IL23 monoclonal antibody

Species Reactivity

Human

Immunogen

Recombinant Human IL12&IL23 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

14D4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The production of the IL12&IL23 recombinant monoclonal antibody involves several steps: separating B cells from the spleen of the immunized animal and using recombinant human IL12&IL23 protein as the immunogen during the immunization process. RNA was isolated from the B cells and converted into cDNA. With the cDNA as the template, the IL12&IL23 antibody-encoding gene was amplified through PCR and cloned into the vector. The recombinant vector was transfected into host cells for antibody expression. The IL12&IL23 recombinant monoclonal antibody was harvested from the cell culture supernatant and purified using affinity chromatography. The antibody was validated by demonstrating its reactivity with human IL12 and IL23 proteins in ELISA.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
If CAPTCHA verification fails, click to refresh the CAPTCHA and try again.

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address