IMMT Recombinant Monoclonal Antibody

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Code	CSB-RA623101MA1HU
Size	$49.9
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Image	IHC image of CSB-RA623101MA1HU diluted at 1:50 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA623101MA1HU diluted at 1:50 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing A431 cells stained with CSB-RA623101MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

Q16891

Target Names

IMMT

Alternative Names

Cell proliferation inducing protein 52 antibody; Cell proliferation-inducing gene 4/52 protein antibody; Heart muscle protein antibody; HMP antibody; IMMT antibody; IMMT_HUMAN antibody; Inner membrane protein mitochondrial antibody; Inner mitochrondial membrane antibody; MICOS complex subunit MIC60 antibody; MINOS2 antibody; Mitochondrial inner membrane organizing system 2 antibody; Mitochondrial inner membrane protein antibody; Mitofilin antibody; Motor protein antibody; p87 antibody; p87/89 antibody; p89 antibody; pig4 antibody; PIG52 antibody; Proliferation-inducing gene 4 antibody

Species Reactivity

Human

Immunogen

Recombinant Human IMMT protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

14E3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

IMMT, also known as Mitofilin or MIC60, serves as a critical component of the mitochondrial contact site and cristae organizing system (MICOS complex). This inner mitochondrial membrane protein plays an essential role in maintaining cristae architecture and mitochondrial morphology, making it a valuable target for researchers investigating mitochondrial dynamics, cellular metabolism, and proliferation pathways. Its involvement in organizing the inner membrane structure positions IMMT as a key player in understanding how mitochondrial ultrastructure influences cellular function and energy production.

This recombinant monoclonal antibody, clone 14E3, offers the reproducibility and consistency that demanding experimental workflows require. Generated using recombinant technology with a defined sequence, this antibody eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived reagents. The human IgG1 isotype format and affinity-chromatography purification ensure high specificity for your target while minimizing background interference.

Validation studies demonstrate reliable performance across multiple applications. Immunohistochemistry testing in paraffin-embedded human heart and kidney tissues at 1:50 dilution reveals clear detection using citrate buffer antigen retrieval, providing researchers studying cardiac or renal mitochondrial biology with a dependable tool. Flow cytometry analysis in A431 cells confirms intracellular detection capability, with distinct signal separation from isotype control when used at 1:100 dilution following formaldehyde fixation and Triton X-100 permeabilization.

Whether you're exploring mitochondrial organization in tissue sections or analyzing IMMT expression at the single-cell level, this antibody supports diverse experimental approaches within signal transduction research and beyond. The validated performance in both fixed tissues and permeabilized cells offers flexibility for researchers examining IMMT's role in cellular proliferation and mitochondrial function.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays an important role in the maintenance of the MICOS complex stability and the mitochondrial cristae morphology.

Gene References into Functions

Sub-mitochondrial localization of the genetic-tagged mitochondrial intermembrane space-bridging components Mic19, Mic60 and Sam50. PMID: 28808085
PKA-mediated phosphorylation of MIC60 negatively regulates mitochondrial clearance that is initiated by PINK1 and Parkin. PMID: 27153535
Mitofilin-knockdown cells showed decreased mitochondrial membrane potential (DeltaPsim) and intracellular ATP content, which were minimally affected in CHCHD6-knockout cells. PMID: 26530328
Mic60 interacted with mtDNA and was involved in the architecture of mtDNA D-loop region. Taken together, our findings reveal a previously unrecognized important role of Mic60 in mtDNA transcription. PMID: 25612828
Mitofilin, a mitochondria protein, is shown to be related to cardiac hypertrophy for the first time, which enhances our understanding of the role of mitochondria in cardiac hypertrophy. PMID: 24555791
Transgenic overexpression of mitofilin preserves mitochondrial structure, leading to restoration of mitochondrial function and attenuation of cardiac contractile dysfunction in the diabetic heart. PMID: 25463274
APOOL is a cardiolipin-binding component of the Mitofilin/MINOS protein complex. PMID: 23704930
mitofilin distinctly functions in mitochondrial cristae remodeling and controls cytochrome c release during apoptosis. PMID: 23058921
CHCM1/CHCHD6, novel mitochondrial protein linked to regulation of mitofilin and mitochondrial cristae morphology. PMID: 22228767
The mitochondrial dysfunction induced by DISC1 deficiency was partially reversed by coexpression of Mitofilin, confirming a functional link between DISC1 and Mitofilin for the normal mitochondrial function. PMID: 20880836
Data show that the nuclear matrix protein matrin 3, cytoskeletal motor protein HMP, and the circadian clock protein lark were significantly decreased in fetal Down syndrome brain. PMID: 12469345
mitofilin is a critical organizer of the mitochondrial cristae morphology and thus indispensable for normal mitochondrial function PMID: 15647377
role in protein import related to maintenance of mitochondrial structure is suggested; mitofilin helps regulate mitochondrial morphology and four of the associated proteins (metaxins 1 and 2, SAM50 and CHCHD3) have been implicated in protein import PMID: 17624330

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Subcellular Location

Mitochondrion inner membrane; Single-pass membrane protein. Mitochondrion.

Protein Families

MICOS complex subunit Mic60 family

Database Links

HGNC: 6047

OMIM: 600378

KEGG: hsa:10989

STRING: 9606.ENSP00000387262

UniGene: Hs.148559

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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