Product Details

Uniprot No.

Target Names

IRF2

Alternative Names

DKFZp686F0244 antibody; Interferon regulatory factor 2 antibody; IRF 2 antibody; IRF-2 antibody; IRF2 antibody; IRF2_HUMAN antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human IRF2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

5H12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Interferon regulatory factor 2 (IRF2) functions as a transcriptional repressor that counterbalances IRF1-mediated activation of interferon-responsive genes, playing a critical role in immune regulation and cellular homeostasis. Beyond its classical role in interferon signaling, IRF2 has emerged as a significant factor in oncogenesis research, with altered expression patterns observed across multiple cancer types, making it a compelling target for investigators studying tumor immunology and transcriptional regulation.

This recombinant monoclonal antibody, clone 5H12, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and produced recombinantly in rabbit host cells, researchers can expect uniform performance across experiments and between lot numbers, eliminating the variability that can complicate long-term studies or multi-site collaborations. The antibody was raised against a synthetic peptide derived from human IRF2 and has been affinity-purified to ensure high specificity for your target.

Validation studies demonstrate reliable performance across complementary detection platforms. Immunohistochemistry on paraffin-embedded human colorectal cancer tissue reveals clear nuclear staining when used at 1:100 dilution with citrate buffer antigen retrieval, providing researchers studying IRF2 in tumor microenvironments with a validated protocol starting point. Flow cytometry analysis using Jurkat cells confirms intracellular detection capability, with distinct positive signal separation from isotype control, supporting applications in immune cell profiling where quantitative assessment of IRF2 expression is needed.

The unconjugated format provides flexibility for pairing with your preferred secondary detection systems. For immunology researchers investigating interferon signaling pathways, transcriptional regulation in immune cells, or IRF2's emerging roles in cancer biology, this antibody delivers the technical reliability needed to advance your work with confidence.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation.

Gene References into Functions

Data suggest that MIR1290 expression is remarkably upregulated in non-small-cell lung carcinoma tissues compared to adjacent normal lung tissues; IRF2 appears to be a direct target of MIR1290. (MIR1290 = microRNA-1290; IRF2 = interferon regulatory factor-2) PMID: 29275213
These finding suggests that miR-302b inhibits key transcription factors and cytokines by targeting ERBB4, IRF2 and CXCR4, implicating its role in the inhibition of CRI in EC. PMID: 28467773
Our study primarily suggests IRF-2 as a potential prognostic biomarker in colorectal cancer PMID: 28465494
The effects of IRF2 suppresses non-small cell lung cancer by promoting cell apoptosis, inhibiting cell proliferation and migration ability. PMID: 28471447
In a genome wide are study to identify acute kidney injury risk in critically ill patients, the locus on chromosome 4, located 150 kb upstream of IRF2 was identified to regulate immunity pathways related to kidney disease risk gene APOL1. Disruption of IRF-2 has been found to up-regulate the inflammatory response to infection. PMID: 27576016
Study shows that IRF2 knockdown inhibits growth, colony formation of OCI/AML-2, OCI/AML-3, and THP-1 cells. In addition, IRF2 knockdown induces apoptosis of acute myeloid leukemia (AML) cells by regulating apoptotic effectors. Further mechanism analysis shows that INPP4B contributes to the effects of IRF2 on apoptosis and growth of AML cells. Thus, IRF2 serves as an important regulator in AML by targeting INPP4B. PMID: 28579269
miR-450 targets IRF2 and thus supresses lung cancer cell proliferation and invasion. PMID: 27246609
It might play as a tumor suppressor by regulating P53 signaling in gastric cancer. PMID: 26173586
the association of IRF2 with susceptibility to systemic lupus erythematosus PMID: 25285625
IRF2genes were not associated with pancreatitis. 4 variants were found: c.123G>A (novel), c.651C>T, c.744G>A, and c.638C>T, p.P213L. PMID: 25207663
miR-221 directly inhibits the expression of SOCS3 and IRF2. PMID: 24607843
IFN regulatory factor 2 (Irf2) has a regulatory role in trypsinogen5 gene transcription, which is resistant to a major endogenous trypsin inhibitor, Spink3 PMID: 22042864
IRF2 overexpression protects against I/R injury by decreasing IRF1-dependent injury and may represent a novel therapeutic strategy. PMID: 22744333
The results suggested that distinct markers in IRF2 may be associated with atopic dermatitis and eczema herpeticum (which may depend upon ethnic ancestry) and genetic variants in IRF2 may contribute to an abnormal immune response to herpes simplex virus. PMID: 22113474
these results suggest that IRF-2 plays an important role in the tumorigenesis of pancreatic cancer and down-regulation of IRF-2 would be a new treatment target for pancreatic cancer. PMID: 22119988
IRF-2 activates the HPV-16 P97 promoter in vivo. PMID: 20129639
IRF-2 activates RIG-I promoter through ISRE-like site as well as IRF-1 and IFN-alpha stimulation. PMID: 20034464
PTB binding to multiple sites within IRF2 5'UTR leads to a conformational change in the RNA that facilitate binding of other trans-acting factors to mediate internal initiation of translation PMID: 19756143
IRF2 is a potential susceptibility gene for psoriasis. PMID: 14962090
IRF-2 is involved in up-regulation of nonmuscle myosin heavy chain II-A gene expression in cell differentiation PMID: 15496418
In cells transfected with both IRF-2 and p300/CBP-associated factor , IRF-2 associated with endogenous nucleolin. PMID: 16582966
IRF8 cooperates with PU.1 and IRF-2 to activate a composite ets/IRF-cis element in the NF1 promoter. The conserved IRF domain tyrosine in ICSBP/IRF8 is required for interaction with the DNA-bound PU.1-IRF2 heterodimer. PMID: 17200120
IRF-2 regulates NF-kappaB activity via the modulation of NF-kappaB subcellular localization. PMID: 18395009
IRF2 interacts with the SUMO-E3 ligase PIASy and is sumoylated in vivo PMID: 18514056

Hide All

Subcellular Location

Nucleus.

Protein Families

IRF family

Tissue Specificity

Expressed throughout the epithelium of the colon. Also expressed in lamina propria.

Database Links

HGNC: 6117

OMIM: 147576

KEGG: hsa:3660

STRING: 9606.ENSP00000377218

UniGene: Hs.654566

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Code	CSB-RA049800A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA049800A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing jurkat cells stained with CSB-RA049800A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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IRF2 Recombinant Monoclonal Antibody

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