KRT13 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA049199A0HU
Size	US$210
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Image	IHC image of CSB-RA049199A0HU diluted at 1:100 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA049199A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of A-431 cell with CSB-RA049199A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Uniprot No.

P13646

Target Names

KRT13

Alternative Names

47 kDa cytokeratin antibody; CK-13 antibody; CK13 antibody; Cytokeratin 13 antibody; Cytokeratin-13 antibody; K13 antibody; K1C13_HUMAN antibody; Ka13 antibody; Keratin 13 antibody; Keratin antibody; keratin type I cytoskeletal 13 antibody; Keratin-13 antibody; Krt-1.13 antibody; Krt1-13 antibody; KRT13 antibody; MGC161462 antibody; MGC3781 antibody; type I cytoskeletal 13 antibody; Type I keratin Ka13 antibody; WSN2 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human KRT13 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

22C9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Keratin 13 (KRT13) is a type I intermediate filament protein that serves as a well-established marker of non-keratinizing stratified squamous epithelia. Expressed predominantly in the suprabasal layers of mucosal tissues, KRT13 plays essential roles in maintaining epithelial integrity and cellular architecture. Its expression patterns make it particularly valuable for researchers investigating epithelial differentiation, tissue homeostasis, and pathological changes in squamous cell carcinomas where altered keratin profiles often accompany disease progression.

This recombinant monoclonal antibody, clone 22C9, offers the reproducibility that demanding research applications require. Developed using recombinant technology with a defined sequence, this antibody eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The rabbit IgG format, raised against a synthetic peptide derived from human KRT13, provides consistent performance across experiments while the affinity-chromatography purification ensures high specificity for your target.

Validation studies demonstrate robust performance in both immunohistochemistry and immunofluorescence applications. In paraffin-embedded human skin tissue, the antibody produces clear staining at 1:100 dilution using citrate buffer antigen retrieval, confirming its utility for examining normal epithelial architecture. Testing in human cervical cancer tissue under identical conditions reveals reliable detection in pathological specimens, supporting its application in oncology research. Immunofluorescence studies in A-431 cells, a human epidermoid carcinoma line, further validate the antibody's performance in cell-based assays at 1:50 dilution.

Whether you are characterizing epithelial differentiation markers, investigating squamous cell carcinoma biology, or establishing tissue-specific expression profiles, this antibody provides the technical consistency needed for meaningful, reproducible results in your research workflow.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Gene References into Functions

Oral white sponge nevus may manifest variable clinical features. The novel mutation found in the KRT13 gene is believed to add evidence for a mutational hotspot in the mucosal keratins PMID: 29476668
this study reports that KRT13 plays a directive role in prostate cancer bone, brain, and soft tissue metastases PMID: 27835867
Keratin 13 gene is epigenetically suppressed during TGFB1-induced epithelial-mesenchymal transition in a human keratinocyte cell line. PMID: 29326042
Four rare missense variants were identified (ACTBL2 rs73757391 (5q11.2), BTD rs200337373 (3p25.1), KRT13 rs150321809 (17q21.2) and MC2R rs104894658 (18p11.21)), but only MC2R rs104894668 had a large effect size (OR = 9.66). PMID: 27378695
KRT13 expression is associated with poor prognosis at multiple stages of disease progression PMID: 27711225
Loss of CK13 expression is associated with invasive oral squamous cell carcinoma. PMID: 26225699
Low KRT13 mRNA expression is associated with oral squamous cell carcinoma. PMID: 25735388
Decreased KRT13 was associated with Esophageal Squamous Cell Carcinoma. PMID: 25851906
Our data provide mechanistic insights into the epigenetic silencing of KRT13 genes in OSCC cells and might be useful for the development of diagnostic markers and novel therapeutic approaches against OSCCs. PMID: 25527207
keratin14 expression can be used to detect early epithelial dysplasia, and that keratin13 and keratin17 expression are useful for detecting neoplastic changes PMID: 24471966
Immunoexpression of CK13 and CK17 in light green-stained superficial cells was associated with more severe morphological atypia in tongue squamous cell carcinoma PMID: 24247036
Fluorescent keratin 13 integrates into the endogenous keratin cytoskeleton in human vulva carcinoma-derived A431 cells, thereby serving as a reliable marker of keratin dynamics. PMID: 23757496
Loss of K13 in oral carcinoma in situ is partly due to its gene repression, but may also be due to some unknown post-translational events. PMID: 22301701
Keratin 13 is a more specific marker of conjunctival epithelium than keratin 19. PMID: 21738394
CK13 expression is elevated in KB cells treated with all-trans retinoic acid or arsenic trioxide. PMID: 19489262
exhibits a regular expression pattern in luminal epithelial cells of secretory phase human endometrium PMID: 11906920
Metaplastic squamous cells differentiate with a decrease of CK18 and an increase of CK13 expression. PMID: 16836912
CK13 positive staining occurs in cervical squamous tumors and in mucinous cervical type adenocarcinomas, while there was no positive staining in non-neoplastic cervical glandular elements PMID: 16949723
The different ligand regulations of KRT13 are due to ligand-differential recruitment of ER and coactivators. PMID: 18951949

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Involvement in disease

White sponge nevus 2 (WSN2)

Protein Families

Intermediate filament family

Tissue Specificity

Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta.

Database Links

HGNC: 6415

OMIM: 148065

KEGG: hsa:3860

STRING: 9606.ENSP00000246635

UniGene: Hs.654550

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Estrogen signaling pathway

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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