MMP17 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA932884A0HU
Size	US$210
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Image	IHC image of CSB-RA932884A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing A431 cells stained with CSB-RA932884A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q9ULZ9

Target Names

MMP17

Alternative Names

Matrix metalloproteinase 17 antibody; Matrix metalloproteinase 17 membrane inserted antibody; Matrix metalloproteinase-17 antibody; Membrane type 4 matrix metalloproteinase antibody; Membrane type matrix metalloproteinase 4 antibody; Membrane-type matrix metalloproteinase 4 antibody; Membrane-type-4 matrix metalloproteinase antibody; MMP 17 antibody; MMP-17 antibody; Mmp17 antibody; MMP17_HUMAN antibody; MT MMP 4 antibody; MT MMP4 antibody; MT-MMP 4 antibody; MT4 MMP antibody; MT4-MMP antibody; MT4MMP antibody; MTMMP 4 antibody; MTMMP4 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human MMP17 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

3G3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

MMP17, also known as membrane-type 4 matrix metalloproteinase (MT4-MMP), represents a distinctive member of the membrane-anchored MMP family that plays important roles in extracellular matrix remodeling and cellular signaling processes. Unlike secreted MMPs, MT4-MMP is tethered to the cell surface through a glycosylphosphatidylinositol anchor, positioning it to regulate pericellular proteolysis in cardiovascular tissues and other biological contexts. This unique localization makes MMP17 a compelling target for researchers investigating vascular biology, tissue remodeling, and related pathological processes.

This recombinant monoclonal antibody, clone 3G3, offers the reproducibility and consistency that demanding experimental workflows require. Developed using recombinant technology with a defined sequence, this rabbit IgG eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The antibody was raised against a synthesized peptide derived from human MMP17 protein and purified by affinity chromatography to ensure specificity.

Validation across multiple platforms demonstrates this antibody's versatility in your research applications. Immunohistochemical staining of paraffin-embedded human brain tissue at 1:100 dilution reveals clear target detection using standard citrate buffer antigen retrieval on automated staining systems. Flow cytometry analysis using A431 cells confirms reliable performance in single-cell applications, with distinct positive signal separation from isotype control when used at 1:100 dilution on fixed and permeabilized cells.

Whether you are investigating MMP17's contributions to cardiovascular remodeling, examining its expression patterns across human tissues, or characterizing its role in cellular processes, this antibody provides a dependable tool with validated performance across immunohistochemistry and flow cytometry applications.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Endopeptidase that degrades various components of the extracellular matrix, such as fibrin. May be involved in the activation of membrane-bound precursors of growth factors or inflammatory mediators, such as tumor necrosis factor-alpha. May also be involved in tumoral process. Cleaves pro-TNF-alpha at the '74-Ala-|-Gln-75' site. Not obvious if able to proteolytically activate progelatinase A. Does not hydrolyze collagen types I, II, III, IV and V, gelatin, fibronectin, laminin, decorin nor alpha1-antitrypsin.

Gene References into Functions

MT4-MMP targeting may constitute a novel strategy to boost patrolling monocyte activity in early inflammation. PMID: 29500407
Three forms of MT4-MMP with molecular masses of 45 kDa, 58 kDa and 69 kDa were detected. Further, we demonstrate that the 58 kDa form is the mature protein in the cell membrane, while the 69 kDa form is its precursor found in intracellular compartments. PMID: 28531887
Low MT4-MMP expression is associated with erlotinib resistance in breast cancer. PMID: 28196064
The MT4-MMP is internalized by the clathrin-independent carriers/GPI-enriched early endosomal compartments pathway, a mechanism that differs from that responsible for the internalization of other membrane-type MMP members. PMID: 26663028
Screening of patients with inherited thoracic aortic aneurysms and dissections identified a missense mutation (R373H) in the MMP17 gene that prevented the expression of the protease in human transfected cells. PMID: 25963716
A functional link between MT4-MMP and the growth factor receptor EGFR. PMID: 25320013
CAV1 when found in lipid rafts does not allow the metalloproteinase MT4-MMP to localize into the lipid rafts, affecting its expression in the cell and probably its activity which is translated into the metastasis-associated activities of these cells. PMID: 22674854
It identifies MT4-MMP as a key intrinsic tumor cell determinant that contributes to the elaboration of a permissive microenvironment for metastatic dissemination. PMID: 22262494
MT4-MMP promotes lung metastasis by disturbing the tumour vessel integrity and thereby facilitating tumour cell intravasation PMID: 19426156
The data presented here provide a new insight into the characteristics of MT4-MMP and highlight the common and distinct properties of the glycosylphosphatidylinositol-anchored membrane type-matrix metalloproteinases. PMID: 21828052
Studies suggest a model of hypoxia induced metastasis through expression of HIF-1alpha, and SLUG regulation of MT4-MMP transcription. PMID: 20019845
Data show that eosinophils constitutively express membrane type-4 matrix metalloproteinase (MT4-MMP), which is increased upon stimulation with tumor necrosis factor-alpha. PMID: 12962706
MT4-MMP and the proteoglycan form of syndecan-1 have roles in ADAMTS-4 activation on the cell surface PMID: 14701864

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Subcellular Location

[Isoform Long]: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. Secreted, extracellular space, extracellular matrix.

Protein Families

Peptidase M10A family

Tissue Specificity

Expressed in brain, leukocytes, colon, ovary testis and breast cancer. Expressed also in many transformed and non-transformed cell types.

Database Links

HGNC: 7163

OMIM: 602285

KEGG: hsa:4326

STRING: 9606.ENSP00000353767

UniGene: Hs.709245

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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