MRPS15 Recombinant Monoclonal Antibody

MRPS15 (mitochondrial ribosomal protein S15) serves as an essential component of the 28S small subunit of the mitochondrial ribosome, playing a fundamental role in the translation of mitochondrial-encoded proteins critical for oxidative phosphorylation and cellular energy metabolism. Research into MRPS15 and other mitochondrial ribosomal proteins has gained significant attention for understanding mitochondrial dysfunction in cancer, metabolic disorders, and aging-related pathologies.

This recombinant monoclonal antibody, clone 9B11, offers researchers the reproducibility and consistency that comes with sequence-defined production. Unlike traditional hybridoma-derived antibodies, recombinant technology ensures that every lot performs identically, eliminating the variability that can compromise longitudinal studies or multi-site collaborations. The antibody was raised in rabbit against a synthesized peptide derived from human MRPS15, providing defined epitope targeting for human samples.

Validation across multiple detection platforms demonstrates this antibody's versatility in your experimental workflows. Immunohistochemistry studies using paraffin-embedded human pancreatic cancer tissue at 1:100 dilution revealed clear target detection following citrate buffer antigen retrieval. Immunofluorescence analysis in HepG2 hepatocellular carcinoma cells at 1:50 dilution showed specific staining patterns when counter-stained with DAPI. Flow cytometry validation in HeLa cells confirmed robust signal separation from isotype control, with clear positive peak shifts observed at 1:100 dilution.

The antibody is supplied in a glycerol-containing buffer optimized for long-term storage stability at -20°C or -80°C. For researchers investigating mitochondrial biology, cancer metabolism, or epigenetics and nuclear signaling pathways, this affinity-purified reagent provides a reliable tool for exploring MRPS15 expression and localization across diverse experimental contexts.