MTA2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA568219A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Jurkat whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg) All lanes: MTA2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 75 kDa Observed band size: 70 kDa Exposure time：3min IHC image of CSB-RA568219A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA568219A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of A431 cell with CSB-RA568219A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing jurkat cells stained with CSB-RA568219A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

O94776

Target Names

MTA2

Alternative Names

Metastasis-associated protein MTA2 (Metastasis-associated 1-like 1) (MTA1-L1 protein) (p53 target protein in deacetylase complex), MTA2, MTA1L1 PID

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide from human MTA2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

11D11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

MTA2 (Metastasis-associated protein 2) serves as a critical component of the nucleosome remodeling and deacetylase (NuRD) complex, where it functions as a key regulator of gene expression through chromatin modification. Originally identified as a p53 target protein in deacetylase complexes, MTA2 plays significant roles in transcriptional repression and has been implicated in cancer progression, making it an important target for researchers investigating epigenetic regulation, tumor biology, and metastatic mechanisms.

This recombinant monoclonal antibody, clone 11D11, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined sequences rather than traditional hybridoma methods, researchers benefit from lot-to-lot uniformity that supports longitudinal studies and ensures comparable results across experiments. The antibody is raised in rabbit against a synthetic peptide derived from human MTA2 and demonstrates cross-species reactivity with both human and mouse samples.

Validation studies confirm robust performance across multiple applications. Western blot analysis detects MTA2 at approximately 70 kDa in both Jurkat and NIH/3T3 whole cell lysates, with the slight difference from the predicted 75 kDa molecular weight likely attributable to alternative splicing/post-translational splicing. Immunohistochemistry staining has been successfully performed on paraffin-embedded human glioma and small intestine tissues using citrate buffer antigen retrieval. The antibody also performs well in immunofluorescence applications, as demonstrated in A431 cells, and flow cytometry analysis of Jurkat cells shows clear positive signal separation from isotype controls.

This versatile reagent supports diverse research applications in chromatin biology, cancer epigenetics, and studies examining NuRD complex function in transcriptional regulation.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

May be involved in the regulation of gene expression as repressor and activator. The repression might be related to covalent modification of histone proteins.

Gene References into Functions

This study is the first to demonstrate that SNHG5 is a critical and powerful regulator that is involved in GC progression through trapping MTA2 in the cytosol. PMID: 27065326
Taken together with previous findings this suggests, that MTA-RBBP is a stable complex, with a central role in the initial assembly of the human NuRD complex. PMID: 28179136
These results indicated there might be a tight correlation among MTA2, Ki-67 and hepatocellular carcinoma prognosis PMID: 26722504
MTA2 is a crucial biomarker that is closely related with prognosis of colorectal carcinoma and also a potential molecular target for evaluating the prognosis and treatment of CRC. PMID: 26261611
MTA2 overexpression enhances colony formation and tumor growth of gastric cancer cells, but not plays important role in cancer cell migration and metastasis. IL-11 is one of the downstream effectors of MTA2 in regulating gastric cancer cells growth PMID: 25929737
MTA-2 protein may facilitate the invasive potential of non-small-cell lung cancer cells through the inhibition of the cell adhesion molecule Ep-CAM and E-cadherin, suggesting that it might be a potential therapeutic target in NSCLC. PMID: 25969565
High MTA2 protein and mRNA expression is associated with glioma. PMID: 25048531
MTA2 acts as a central hub for cytoskeletal organization and transcription and provides a link between nuclear and cytoskeletal organization. PMID: 25394532
MTA2 depletion could significantly inhibit human breast cancer cell growth and metastasis, implying that MTA2 might be involved in the progression of breast cancer. PMID: 25081667
Study demonstrates that hBD-3 inhibits the progression of colon cancer in a paracrine fashion through downregulation of MTA2 expression. PMID: 24969834
Mta2 and Tipin cooperate to maintain replication fork integrity, especially on regions that are intrinsically difficult to duplicate. PMID: 24830473
MTA2 knockdown impairs invasion and metastasis of gastric cancer cells. PMID: 24010737
results reveal a novel post-translational regulation of MTA2 by the way of p300-dependent acetylation, which is important for tumor cells growth and migration and provides a potential target for clinical cancer research PMID: 24468085
High expression levels of MTA2 is associated with pancreatic ductal adenocarcinoma. PMID: 23400716
MTA2 is highly expressed in the primary lesions of gastric cancer than that in adjacent non-cancerous tissues, and is closely related with tumor invasion. MTA2 expression is elevated in Sp1 positive gastric cancer. PMID: 23158992
Sertoli cell-specific expression of MTA2 is required for transcriptional regulation of FSHR gene during spermatogenesis. PMID: 23086931
High nuclear MTA2 expression is associated with thymoma. PMID: 22833212
Our data suggested that MTA2 might play roles in both the nucleus and cytoplasm in the progression of NSCLC. PMID: 22585429
The expression of MTA2 had positive correlation with clinical stage and lymph node metastasis. PMID: 20704817
interaction with mCpG-binding domain of MBD2 PMID: 12124384
MTA2 is a repressor of estrogen receptor (ER)alpha activity and that it could represent a new therapeutic target of ERalpha action in human breast tumors PMID: 16645043
Findings indicate a tight correlation between the MTA2 expression level and hepatocellular carcinoma size and differentiation. PMID: 19702911
Deacetylation and functional interactions by the PID/MTA2-associated NuRD complex may represent an important pathway to regulate p53 function. PMID: 11099047

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Subcellular Location

Nucleus.

Tissue Specificity

Widely expressed.

Database Links

HGNC: 7411

OMIM: 603947

KEGG: hsa:9219

STRING: 9606.ENSP00000278823

UniGene: Hs.173043

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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