MYBBP1A Recombinant Monoclonal Antibody

Code CSB-RA978157A0HU
Size US$210
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  • IHC image of CSB-RA978157A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • Immunofluorescence staining of HepG2 Cells with CSB-RA978157A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Product Details

Uniprot No.
Target Names
MYBBP1A
Alternative Names
Myb-binding protein 1A, MYBBP1A, P160
Species Reactivity
Human
Immunogen
A synthesized peptide derived from human MYBBP1A
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
3A1
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:50-1:200
IF 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

To create the MYBBP1A recombinant monoclonal antibody, several steps are necessary. These include the harvest of the MYBBP1A monoclonal antibody, MYBBP1A monoclonal antibody gene sequencing, vector construction, and transfection of the constructed MYBBP1A monoclonal antibody gene-carrying vector into a host cell line for culture. The synthesized peptide from human MYBBP1A is used as an immunogen in the MYBBP1A monoclonal antibody production. The MYBBP1A recombinant monoclonal antibody is purified from the cell culture supernatant, and its specificity is evaluated through ELISA, IHC, and IF tests. It only reacts with human MYBBP1A protein.

The MYBBP1A is a transcriptional co-regulator that plays important roles in the regulation of gene expression, cell cycle progression, DNA repair, and apoptosis. MYBBP1A is involved in the regulation of gene expression by acting as a transcriptional co-regulator. It plays a role in the regulation of the cell cycle by interacting with E2F transcription factors. MYBBP1A interacts with the DNA repair protein Ku70 and is required for efficient non-homologous end-joining (NHEJ) repair of DNA double-strand breaks. Dysregulation of MYBBP1A activity has been implicated in various diseases, including cancer and neurological disorders.

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Target Background

Function
May activate or repress transcription via interactions with sequence specific DNA-binding proteins. Repression may be mediated at least in part by histone deacetylase activity (HDAC activity). Acts as a corepressor and in concert with CRY1, represses the transcription of the core circadian clock component PER2. Preferentially binds to dimethylated histone H3 'Lys-9' (H3K9me2) on the PER2 promoter. Has a role in rRNA biogenesis together with PWP1.
Gene References into Functions
  1. The results indicated that both the heterozygous genotype GC and homozygous genotype CC in rs3809849 in MYBBP1A had significant effects on the risk of pulmonary tuberculosis, and heterozygous genotype CT in rs9061 in SP110 also had similar effects. PMID: 25612917
  2. We propose that the nucleolus functions as a stress sensor to modulate p53 protein levels and its acetylation status, determining cell fate between cell cycle arrest and apoptosis by regulating MYBBP1A translocation. PMID: 26044764
  3. MYBBP1A(low)AKT(Ser473)(high) staining pattern serves not only as a marker for the pre-senescent stage but also as an indicator of OPSCC patients at high risk for treatment failure. PMID: 25543088
  4. TPPII, MYBBP1A and CDK2 form a protein-protein interaction network. PMID: 25303791
  5. MYBBP1A functions to enhance p53 tetramerization that is necessary for p53 activation. PMID: 24375404
  6. Mybbp1a is a novel negative regulator of Sirt7. PMID: 24134843
  7. Regulators, including BCL11A, MYB, and KLF1, hold great promise to develop targeted and more effective approaches for HbF induction. PMID: 23209159
  8. MYBBP1A-p53 binding property can account for efficient p53-activation by MYBBP1A under nucleolar stress. Our results support the idea that MYBBP1A plays catalytic roles in p53 acetylation and activation PMID: 23583237
  9. Suggest that MYBBP1A is required for p53 activation during anoikis; therefore, it is involved in suppressing colony formation and the tumorigenesis of breast cancer cells. PMID: 23388179
  10. Data show that down-regulation of MYBBP1A decreases the growth rate of wild type mouse embryonic stem cells, embryo fibroblasts (MEFs) and of human HeLa cells, where it also promotes apoptosis. PMID: 23056166
  11. Study suggests that a combination of SP110 and MYBBP1A gene polymorphisms may serve as a novel marker for identifying the risk of developing TB in the Chinese Han population. PMID: 23129390
  12. Results from our present work reveal a previously unrecognized co-repressor role of Mybbp1a in rRNA expression. PMID: 22686419
  13. Mybbp1a may play a dual role in the rRNA metabolism, potentially linking and coordinating ribosomal DNA transcription and pre-rRNA processing to allow for the efficient synthesis of ribosomes. PMID: 22645127
  14. We provide experimental evidence that MYBBP1A is an important molecular switch in the regulation of tumor cell proliferation versus migration in head and neck squamous cell carcinoma cells. PMID: 22339894
  15. When rRNA transcription was suppressed by nucleolar stress, MYBBP1A translocated to the nucleoplasm and facilitated p53-p300 interaction to enhance p53 acetylation. PMID: 21297583
  16. identified MYBBP1A as a novel Aurora B substrate and serine 1303 as the major phosphorylation site PMID: 20177074
  17. Myb-binding protein 1a was identified in anti-SMN pulldowns as interacting with SMN proteins. PMID: 19928837
  18. These results indicate that MYBBP1a is a novel NF-kappaB co-repressor of transcription that competes with p300 and may function to regulate cell type specific genes. PMID: 17196614
  19. Expression of Ets-2, SRC-1 and c-Myc individually are all associated with reduced disease-free survival in breast cancer PMID: 18059336
  20. SRC-1 is a strong independent predictor of reduced disease free survival, whereas the interactions of the p160 proteins with estrogen receptor alpha can predict the response of patients to endocrine treatment. PMID: 19276281

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Subcellular Location
Cytoplasm. Nucleus. Nucleus, nucleolus.
Database Links

HGNC: 7546

OMIM: 604885

KEGG: hsa:10514

STRING: 9606.ENSP00000370968

UniGene: Hs.22824

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