NDUFAB1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA288465A0HU
Size	US$210
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Image	IHC image of CSB-RA288465A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA288465A0HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing Hela cells stained with CSB-RA288465A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Target Background

Product Details

Uniprot No.

O14561

Target Names

NDUFAB1

Alternative Names

ACP antibody; ACPM_HUMAN antibody; Acyl carrier protein antibody; Acyl carrier protein mitochondrial antibody; CI SDAP antibody; CI-SDAP antibody; FASN 2A antibody; FASN2A antibody; MGC65095 antibody; Mitochondrial acyl carrier protein antibody; mitochondrial antibody; NADH dehydrogenase (ubiquinone) 1 alpha/beta subcomplex 1 8kDa antibody; NADH dehydrogenase (ubiquinone) 1 alpha/beta subcomplex 1 antibody; NADH ubiquinone oxidoreductase 9.6 kDa subunit antibody; NADH ubiquinone oxidoreductase SDAP subunit antibody; NADH-ubiquinone oxidoreductase 9.6 kDa subunit antibody; NDUFAB 1 antibody; ndufab1 antibody; SDAP antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human NDUFAB1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

23B9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Carrier of the growing fatty acid chain in fatty acid biosynthesis. Accessory and non-catalytic subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain.

Gene References into Functions

The p49/STRAP and NDUFAB1 proteins interacted and co-localized with each other in the cell. PMID: 18230186
ACP has a dual role in mammalian mitochondrial function PMID: 19221180

Subcellular Location

Mitochondrion.

Protein Families

Acyl carrier protein (ACP) family

Database Links

HGNC: 7694

OMIM: 603836

KEGG: hsa:4706

STRING: 9606.ENSP00000007516

UniGene: Hs.189716

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Oxidative Phosphorylation

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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