Product Details

Uniprot No.

Q96RD7

Target Names

PANX1

Alternative Names

Pannexin-1, PANX1, MRS1

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human PANX1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

28G5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Pannexin-1 (PANX1) serves as a critical membrane channel protein that mediates ATP release and plays essential roles in purinergic signaling, inflammasome activation, and cell death pathways. In neuroscience research, PANX1 has garnered significant attention for its involvement in synaptic plasticity, neuroinflammation, and ischemic injury responses, making it a compelling target for investigators exploring both physiological and pathological brain processes.

This recombinant monoclonal antibody, clone 28G5, offers the reproducibility and consistency that demanding research protocols require. Because recombinant antibodies are produced from defined genetic sequences rather than traditional hybridoma methods, you can expect reliable performance across experiments and between lot numbers—a meaningful advantage when building longitudinal datasets or establishing standardized workflows in your laboratory.

The antibody has been validated across multiple detection platforms, providing flexibility for diverse experimental approaches. Immunohistochemistry studies demonstrate clear PANX1 detection in paraffin-embedded human brain tissue and human lung cancer specimens at dilutions of 1:50, using citrate buffer antigen retrieval. For cellular localization studies, immunofluorescence staining in U251 glioblastoma cells reveals distinct signal patterns when counter-stained with DAPI. Flow cytometry validation using A549 cells confirms the antibody's utility for surface staining applications, with clear separation between specific signal and isotype control populations.

Supplied in a glycerol-containing buffer optimized for long-term stability at -20°C or -80°C, this antibody arrives ready for integration into your existing protocols. Whether you're investigating PANX1's role in neurological disease models, characterizing its expression in tumor microenvironments, or exploring purinergic signaling mechanisms, this reagent provides a dependable foundation for your research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA549607A0HU
Size	US$210
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Image	IHC image of CSB-RA549607A0HU diluted at 1:50 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.1:50% DAB. IHC image of CSB-RA549607A0HU diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.1:50% DAB. Immunofluorescence staining of U251 with CSB-RA549607A0HU at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 502-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing A549 cells surface stained with CSB-RA549607A0HU (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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PANX1 Recombinant Monoclonal Antibody

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