Product Details

Uniprot No.

Target Names

POU2F2

Alternative Names

POU domain, class 2, transcription factor 2 (Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2) (Octamer-binding protein 2) (Oct-2) (Octamer-binding transcription factor 2) (OTF-2), POU2F2, OCT2 OTF2

Species Reactivity

Human

Immunogen

A synthesized peptide from human POU2F2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

13B1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, FC

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Protocols

ELISA Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

POU2F2, also known as Oct-2, is a POU domain transcription factor that plays a critical role in B-cell development and immunoglobulin gene expression. As a lymphoid-restricted octamer-binding protein, POU2F2 regulates the transcription of immunoglobulin genes by binding to conserved octamer motifs in their promoter and enhancer regions. This makes it an essential target for researchers investigating B-cell biology, lymphocyte differentiation, and the molecular mechanisms underlying humoral immunity.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human POU2F2, offers the consistency and reliability that demanding experimental workflows require. Because the antibody sequence is defined and produced recombinantly in rabbit host cells, researchers benefit from exceptional lot-to-lot reproducibility, eliminating the variability often encountered with traditional hybridoma-derived antibodies. The monoclonal nature of clone 13B1 ensures specificity for a single epitope, providing clean and interpretable results across experiments.

Validated for flow cytometry applications, this antibody has been successfully tested on Raji cells, a well-established human B-lymphocyte cell line, at dilutions ranging from 1:50 to 1:200. The validation protocol employed fixation and permeabilization to access the nuclear transcription factor, demonstrating clear positive signal separation from isotype control, with acquisition of over 10,000 events confirming robust detection.

Supplied in a glycerol-containing buffer optimized for long-term storage stability, this affinity-purified antibody is well-suited for researchers studying B-cell transcriptional programs, lymphoma biology, or immune cell differentiation pathways where precise detection of POU2F2 expression levels is essential.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA225367A0HU
Size	US$210
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Image	Overlay Peak curve showing Raji cells stained with CSB-RA225367A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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POU2F2 Recombinant Monoclonal Antibody

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