Product Details

Uniprot No.

Target Names

RARB

Alternative Names

HAP antibody; HBV-activated protein antibody; NR1B2 antibody; Nuclear receptor subfamily 1 group B member 2 antibody; RAR B antibody; RAR beta antibody; RAR epsilon antibody; RAR-beta antibody; RAR-epsilon antibody; RARB antibody; RARB_HUMAN antibody; Retinoic acid receptor beta 2 antibody; Retinoic acid receptor beta 4 antibody; Retinoic acid receptor beta 5 antibody; Retinoic acid receptor beta antibody; Retinoic acid receptor beta polypeptide antibody; RRB2 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human RARB protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

18B11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, FC

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Protocols

ELISA Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Retinoic acid receptor beta (RARB) functions as a ligand-dependent transcription factor that mediates the biological effects of retinoids, playing essential roles in cellular differentiation, proliferation, and apoptosis. As a member of the nuclear receptor superfamily, RARB regulates gene expression programs critical to development and has garnered significant attention in cancer research, where its epigenetic silencing is frequently observed across multiple tumor types. Understanding RARB expression and activity provides valuable insights into retinoid signaling pathways and their therapeutic potential.

This recombinant monoclonal antibody, clone 18B11, offers the reproducibility and consistency that rigorous research demands. Developed using recombinant technology with a defined sequence, it eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies, ensuring your experimental results remain comparable across studies and over time. The rabbit IgG format, raised against a synthesized peptide from human RARB protein, has been affinity-purified to deliver reliable specificity for your target.

Validation studies confirm this antibody performs effectively in flow cytometry applications, with recommended dilutions ranging from 1:50 to 1:200. Testing in A431 human epidermoid carcinoma cells demonstrated clear positive staining compared to isotype controls, with cells fixed in formaldehyde and permeabilized with Triton X-100 to enable detection of this nuclear receptor. The antibody is also validated for ELISA, providing flexibility for researchers requiring multiple detection platforms.

For investigators exploring epigenetics, nuclear signaling, or retinoid biology, this RARB antibody delivers the technical performance needed to advance understanding of this important transcriptional regulator and its role in normal physiology and disease states.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. The RXRA/RARB heterodimer can act as a repressor on the DR1 element and as an activator on the DR5 element. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function.

Gene References into Functions

Hypermethylation of RARB gene is associated with high grade lung cancer. PMID: 29851970
RAR-beta expression state is one of the independent factors for the prognosis of lung squamous cell carcinoma patients PMID: 29077166
Although studies reported different rates for RAR beta promoter methylation in prostate can tissues, the total meta-analysis demonstrated that RAR beta promoter methylation may be correlated with prostate cancer carcinogenesis and that the RAR beta gene is particularly susceptible. PMID: 29554659
Hypermethylation frequency of CpG islands in the promoters of retinoic acid receptor beta gene (RAR-beta) gene was respectively 61 and 33% for diffuse and intestinal type n gastric cancer tissues. PMID: 28759951
There were significant associations between RARB promoter hypermethylation and Oral Cancer risk. PMID: 29538221
Results indicate that RARbeta hypermethylation correlates well with an increased risk in non-small cell lung cancer (NSCLC) patients. RARbeta gene inactivation caused by RARbeta methylation contributes the NSCLC tumorigenesis and may serve as a potential risk factor, diagnostic marker and drug target of NSCLC. [review] PMID: 28008143
retinoic acid receptor beta-retinoic X receptor alpha heterodimer quaternary architecture variable PMID: 29021580
Data shows that miR-106a directly targets RARB 3'-UTR and the miR-106a-RARB complex promotes the viability of thyroid cancer. PMID: 27342319
Study identified a novel mutation in RARB gene in patients with intellectual disability and progressive motor impairment which confers gain-of-function further promoting the retinoic acid (RA) ligand-induced transcriptional activity by twofold to threefold over the wild-type receptor. These results providing novel insight into the role of RA in neural networks in humans. PMID: 27120018
mRNAs expression and methylation pattern of RARB, NR4A1 and HSD3B2 genes in human adrenal tissues (HAT) and in pediatric virilizing adrenocortical tumors (VAT) were analyzed. PMID: 27670690
RARb and FHIT promoter methylation may be associated with the carcinogenesis of cervical cancer. FHIT promoter methylation may play a crucial role in cervical cancer progression. Additional studies with large sample sizes are essential to confirm our findings. PMID: 28639889
Upregulation of RARB enhances the sensitivity of cholangiocarcinoma cells to chemotherapeutic agents in vitro. PMID: 27599527
Lack of RARbeta nuclear expression is associated with Non-small cell lung cancer development and associated with a worse prognosis. PMID: 27306217
methylated promoters of DAPK1 combined with MGMT, MGMT combined with RARB, DAPK1 combined with RARB were positive correlated with cervical disease grade PMID: 26823825
Overexpression of miR-146a-5p and miR-146b-5p caused a 31% and 33% decrease in endogenous RARB mRNA levels. PMID: 27011326
shows that RAR-beta methylation detected in lung tissue may be used as a predictive marker for non-small cell lung cancer diagnosis and that APC methylation in tumor sample may be a useful marker PMID: 26681652
RARbeta2 methylation is significantly increased in breast cancer samples when compared to non-cancerous controls PMID: 26451736
Whole-exome sequencing in a South American cohort links ALDH1A3, FOXN1 and RARB/retinoic acid regulation pathways to autism spectrum disorders. PMID: 26352270
Hypermethylation at promoters of RARB, Dietary folate and cobalamin intake is inversely associated with methylated RARB and BRCA1. High dietary intake of riboflavin and pyridoxine is associated with increased methylation in the RARB promoter. PMID: 25805039
Hypermethylation of RARB was associated with esophageal squamous cell carcinoma. PMID: 25514805
Retinoic acid receptor beta and angiopoietin-like protein 1 are involved in the regulation of human androgen biosynthesis PMID: 25970467
Methylated APC and RARbeta2 genes might be valuable serum-based molecular markers for early detection of breast cancer. PMID: 25684670
Curcumin upregulates RARbeta by inhibiting DNMT3b expression which reverses RARbeta promoter methylation. PMID: 25981383
revealed that HSP70 could directly regulate retinoid acid (RA)-induced retinoid acid receptor beta2 (RARbeta2) gene transcription through its binding to chromatin, with R469me1 being essential in this process PMID: 26080448
Methylation of the RAR-beta gene with cigarette smoking is associated with non-small cell lung cancer. PMID: 25605205
Data indicate a differential sensitivity of chondral versus endochondral differentiation pathways to retinoic Acid receptor-Beta (RARbeta) signaling. PMID: 24851851
RAR-beta, whereas ATRA alone had no effect on methylation. The RAR-beta gene was reexpressed following DAC-ATRA combination treatment, and both agents had no effect on p16 expression. PMID: 25413479
in the absence of retinoid signaling via RARbeta, reduced IGF-1 signaling results in suppression of epithelial-mesenchymal transition and delays tumorigenesis induced by the Wnt1 oncogene PMID: 25422594
promoter methylation may play an important role in carcinogenesis of the NSCLC. PMID: 24796328
RARbeta2 circulating DNA in lung cancer patients is at greater methylation rate than in healthy donors. PMID: 25110096
The total frequency of FHIT, RASSF1A and RARbeta gene methylation was significantly higher in lung cancer. PMID: 25040980
Analysis indicates that the genes BIRC5, HOXA1 and RARB are critical targets that play an important regulatory role in cervical cancer pathogenesis. PMID: 25069511
Our meta-analyses identified strong associations of four aberrantly methylated genes (GSTP1, RASSF1, p16, and RARB) with Prostate cancer PMID: 25053593
Results suggest that epigenetic alterations of P16INK4A (p16) and retinoic acid receptor beta (RAR-beta) have an important role in ovarian carcinogenesis. PMID: 24913706
RARB and STMN2 polymorphisms were not associated with sporadic CJD in the Korean population. PMID: 24414001
RARbeta promoter is hypermethylated in all studied prostate tumors and methylation levels are positively correlated with H3K27me3 enrichments. PMID: 24492483
These results highlight the relevance of MSK1 in the up-regulation of RARbeta by prostaglandin E2. PMID: 24953041
The data suggest that RARbeta and p14ARF may be linked in a signaling pathway that is operating in melanocytic cells and is lost in the majority of melanomas. PMID: 23851445
Differences in RAR and RXR subtype mRNA expression patterns in various PTCs may contribute to the immunochemistry data available, and may thus find exploitation in clinical oncology, particularly in the differential diagnosis of thyroid neoplasms PMID: 23969901
Both recessive and dominant mutations in RARB cause anophthalmia. PMID: 24075189
RARbeta2 methylation is associated with the risk of developing prostate cancer. (Meta-analysis) PMID: 23675444
The highest loss of heterozygosity and/or microsatellite imbalance is revealed in RARB locus in non-small cell lung cancer. PMID: 23504373
HHIP, HDAC4, NCR3 and RARB polymorphisms may have a role in impaired lung function that begins in early life PMID: 23456936
All-trans retinoic acid inhibits human scleral fibroblasts proliferation by a mechanism associated with modulation of ERK 1/2 and JNK activation and depends on stimulation of retinoic acid receptor beta. PMID: 23946634
Methylation of the RARB gene increases prostate cancer risk in black Americans. PMID: 23376149
EGFR transactivation by intracellular PGE2-activated EP receptors results in the sequential activation of RARbeta and HIF-1alpha PMID: 23644172
Data show that TLR3 activation by poly I-C induces up-regulation of microRNA-29b, -29c, -148b, and -152 targeting DNA methyltransferases, leading to demethylation and reexpression of the oncosuppressor retinoic acid receptor beta (RARbeta). PMID: 23716670
findings showed that hepatitis C virus Core induced promoter hypermethylation of RAR-beta2 to inhibit its expression; accordingly, Core-expressing cells exhibited resistance to ATRA-induced growth inhibition PMID: 23474497
Second RARbeta2 loss of expression was found associated with different epigenetic profiles in LNCaP and DU145 cells. PMID: 23299856
HIF-1alpha up-regulation is dependent on intracrine PGE2-RARbeta signalling. PMID: 22964004

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Involvement in disease

Microphthalmia, syndromic, 12 (MCOPS12)

Subcellular Location

Nucleus. Cytoplasm.; [Isoform Beta-1]: Nucleus.; [Isoform Beta-2]: Nucleus.; [Isoform Beta-4]: Cytoplasm.

Protein Families

Nuclear hormone receptor family, NR1 subfamily

Tissue Specificity

Expressed in aortic endothelial cells (at protein level).

Database Links

HGNC: 9865

OMIM: 180220

KEGG: hsa:5915

STRING: 9606.ENSP00000332296

UniGene: Hs.543218

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Code	CSB-RA249444A0HU
Size	US$210
	Order now
Image	Overlay Peak curve showing A431 cells stained with CSB-RA249444A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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RARB Recombinant Monoclonal Antibody

Product Details

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Customer Reviews and Q&A

Target Background