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RYBP Recombinant Monoclonal Antibody

Datasheet
Code CSB-RA250534A0HU
Size US$210
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Image
  • IHC image of CSB-RA250534A0HU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
  • Immunofluorescence staining of SH-SY5Y cell with CSB-RA250534A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • Overlay Peak curve showing A431 cells stained with CSB-RA250534A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Uniprot No.
Q8N488
Target Names
RYBP
Alternative Names
2410018J24Rik antibody; AAP 1 antibody; AAP1 antibody; APAP 1 antibody; APAP-1 antibody; APAP1 antibody; Apoptin associating protein 1 antibody; Apoptin-associating protein 1 antibody; cb337 antibody; Death effector domain associated factor antibody; Death effector domain-associated factor antibody; Death effector domain-associated factor B antibody; DED associated factor antibody; DED-associated factor antibody; DED-associated factor B antibody; DEDAF antibody; MGC62213 antibody; RING1 and YY1 binding protein a antibody; RING1 and YY1 binding protein antibody; RING1 and YY1-binding protein antibody; RING1 and YY1-binding protein B antibody; Ring1 interactor RYBP antibody; RYBP antibody; RYBP_HUMAN antibody; rybpa antibody; wu:fi34f03 antibody; YEAF 1 antibody; YEAF1 antibody; YY1 and E4TF1 associated factor 1 antibody; YY1 and E4TF1-associated factor 1 antibody; YY1- and E4TF1/GABP-associated factor 1 antibody; zgc:153842 antibody
Species Reactivity
Human
Immunogen
A synthesized peptide derived from human RYBP
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
15D5
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, IHC, IF, FC
Recommended Dilution
Application Recommended Dilution
IHC 1:50-1:200
IF 1:50-1:200
FC 1:50-1:200
Protocols
ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

RYBP (RING1 and YY1-binding protein) serves as a critical component of the Polycomb repressive complex 1, where it functions as a key regulator of gene silencing and chromatin remodeling. This multifunctional protein participates in diverse cellular processes including apoptosis, stem cell maintenance, and developmental gene regulation. Its interaction with transcription factor YY1 and involvement in death effector domain signaling pathways make RYBP a compelling target for researchers investigating epigenetic mechanisms, cancer biology, and cellular differentiation.

This recombinant monoclonal antibody, generated from clone 15D5 in rabbit host, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and produced recombinantly, researchers can expect reliable performance across experiments and over time, eliminating the lot-to-lot variability that can complicate longitudinal studies or multi-site collaborations.

Validation across multiple detection platforms demonstrates this antibody's experimental versatility. Immunohistochemistry studies in paraffin-embedded human placenta tissue at dilutions of 1:50–1:200 confirm robust target detection in fixed tissue contexts. Immunofluorescence analysis in SH-SY5Y neuroblastoma cells reveals clear signal with appropriate subcellular localization when used at 1:50. Flow cytometry validation using A431 epidermoid carcinoma cells shows distinct positive population shifts compared to isotype controls, confirming utility for single-cell analysis applications.

The antibody is supplied in a stabilized liquid format containing glycerol for convenient long-term storage at -20°C or -80°C. Whether investigating Polycomb-mediated gene silencing, exploring RYBP's role in apoptotic pathways, or characterizing its function in stem cell biology, this antibody provides a dependable tool for advancing understanding of this important epigenetic regulator.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1-like complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Component of a PRC1-like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females. May stimulate ubiquitination of histone H2A 'Lys-119' by recruiting the complex to target sites. Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. May also regulate the ubiquitin-mediated proteasomal degradation of other proteins like FANK1 to regulate apoptosis. May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. May bind to DNA. May play a role in the repression of tumor growth and metastasis in breast cancer by down-regulating SRRM3.
Gene References into Functions
  1. This review also discusses the role of RYBP in apoptosis through non-epigenetic mechanisms as well as recent investigations linking the role of RYBP to apoptosis and cancer. PMID: 29665352
  2. This study revealed the molecular mechanism responsible for RYBP nuclear translocation. PMID: 27989698
  3. this is the first study to show the correlation between RYBP polymorphisms with the risk and prognosis of HCC. We found that the RYBP SNP, rs2118593 can be a new predictor for poor prognosis of HCC, providing a scientific basis for improving the treatment and prognosis of HCC. PMID: 28369212
  4. findings support the idea that RYBP may represent a target for cancer therapy and suggest that it may be useful as a prognostic biomarker for HCC, either alone or in combination with KLF4 and Sp1 PMID: 28028181
  5. overexpression of RYBP suppresses breast cancer growth and metastasis both in vitro and in vivo. PMID: 27748911
  6. RYBP activates FANK1-mediated activator protein 1 (AP-1) signaling pathway which contributes to tumor cell apoptosis. PMID: 27060496
  7. Results identify a novel YY1 ~ miR-9 ~ RYBP axis involved in melanoma tumorigenesis and reinforce the idea that regulatory circuitries involving miRNAs and TFs are prevalent mechanisms. PMID: 26104682
  8. RYBP has a role in survival of patients with hepatocellular carcinoma (HCC) and responsiveness to chemotherapy PMID: 25344099
  9. Polymorphisms in RYBP and AOAH genes are associated with chronic rhinosinusitis in a Chinese population. PMID: 22723975
  10. interacts specifically with the E2F2 and E2F3 family members, dependent on the marked box domain in these proteins PMID: 12411495
  11. Apoptin is efficiently delivered and induced apoptosis in cancer cells when fused to the HIV-TAT protein transduction domain. PMID: 14691460
  12. DEDAF associates with a DED-containing DNA-binding protein in the nucleus and regulates the transcription activity [DEDAF human] PMID: 14765135
  13. Adenovirus expressing Rybp synergistically cooperates with the death receptor ligand, tumor necrosis factor-alpha, in the induction of apoptosis, and thus may have clinical applicability for the treatment of cancer. PMID: 18551146
  14. RYBP interacts with MDM2 via residues 1-73 and 144-228 & decreases MDM2-mediated p53 ubiquitination, stabilizing p53 increasing its activity. RYBP is decreased in human cancer tissues. PMID: 19098711

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Subcellular Location
Nucleus. Cytoplasm. Nucleus, nucleoplasm.
Tissue Specificity
Down-regulated in breast cancer tissues and in several breast cancer cell lines (at protein level). Widely expressed with highest levels in lymphoid tissues and placenta.
Database Links

HGNC: 10480

OMIM: 607535

KEGG: hsa:23429

STRING: 9606.ENSP00000419494

UniGene: Hs.7910

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