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The production of the SARS-CoV-2 Spike RBD recombinant monoclonal antibody involves the application of DNA recombinant technology and in vitro genetic manipulation. Initially, an animal is immunized with a recombinant human SARS-CoV-2 Spike glycoprotein (S) (319-541aa) (CSB-YP3324GMY1 and CSB-MP3324GMY1b1), resulting in the isolation of B cells. From these B cells, positive B cells are selected for further screening and identification of single clones. The light and heavy chains of the SARS-CoV-2 Spike RBD antibody are then amplified through PCR and inserted into a plasmid vector to create a recombinant vector. This vector is subsequently transfected into a host cell line to facilitate antibody expression. The SARS-CoV-2 Spike RBD recombinant monoclonal antibody is collected and purified from the cell culture supernatant using affinity chromatography. This antibody exhibits reactivity towards human SARS-CoV-2 Spike RBD protein and is highly suitable for ELISA, GICA, and neutralizing applications.
The SARS-CoV-2 Spike RBD (receptor-binding domain) protein is a key component of the virus that enables it to enter host cells. The RBD binds to the human ACE2 receptor on the surface of host cells, initiating the process of viral entry. The interaction between the SARS-CoV-2 Spike RBD and the human ACE2 receptor is the target of several vaccines and therapeutic antibodies aimed at preventing viral entry and reducing the severity of COVID-19.
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Where was CSB-RA33245A2GMY purified from? cell culture / hybridoma or serum / ascites?
What is the concentration of this antibody, do you have this antibody in stock?
I am interested in this product. What is the detection limit? Is it below 100-1000 SARS-CoV-2 particles?
Is there any advantage of Nanobody compared with traditional hybridoma monoclonal antibody?
What's the meaning of "Nanobody"?
What is the research significance of SARS-CoV-2 Spike RBD Nanobody?
In the Colloidal Gold Immunochromatography Assay, the detection limit was as low as 25ng/ml (1.75ng). Does it mean a concentration of 25ng/ml?