The recombinant human ACE2 is prepared by the expression of a target fragment corresponding to 18-740aa of the human ACE2 protein with a human Fc tag at the C-terminus in the mammalian expression system.
The monocarboxypeptidase ACE2, a homolog of ACE, is a negative regulator of the renin-angiotensin-aldosterone system (RAAS). It plays a protective role in the cardiovascular system and many organs by balancing multiple functions of ACE. And ACE2 is also the primary target for SARS-CoV-2 and facilitates viral invasion into the host cells. Binding with an amino acid transporter, ACE2 is conducive to the absorption of amino acids in the kidney and intestines.
This human ACE2 contains endotoxin less than 1.0 EU/ug as determined by the LAL method, and its purity is over 85% as measured by SDS-PAGE. It has high reactivity and binding-affinity with SARS-CoV-2-S1 and SARS-CoV-2-S1-RBD, which were verified in the functional ELISA and LSPR assay, respectively. As is well-known, a recombinant protein tagged with Fc is relatively more stable & productive and efficient to be purified by protein A. And this ACE2 protein carries a hFc-tag, which can bind to the Fc receptor on the surface of immune cells, exerting multiple biological effects.
||Less than 1.0 EU/ug as determined by LAL method.
||①Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S (CSB-MP3324GMY) at 2 μg/ml can bind human ACE2, the EC50 of human ACE2 protein is 56.64 - 103.6 ng/ml
②Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD (CSB-YP3324GMY1) at 5 μg/ml can bind human ACE2, the EC50 of human ACE2 protein is 31.80 - 44.69 ng/ml.
③Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD (CSB-MP3324GMY1b1) at 5 μg/ml can bind human ACE2, the EC50 is 2.785-9.139 ng/ml.
④Measured by its binding ability in a functional ELISA. Immobilized human ACE2 at 2 μg/ml can bind SARS-CoV-2-S1-RBD (CSB-MP3324GMY1), the EC50 of human ACE2 protein is 8.363-12.82 ng/ml.
⑤SARS-CoV-2 Spike protein RBD his/sumostar tag (CSB-YP3324GMY1) captured on COOH chip can bind Human ACE2 protein Fc tag (CSB-MP866317HU) with an affinity constant of 100 nM as detected by LSPR Assay.
⑥SARS-CoV-2 Spike protein RBD his/myc tag (CSB-MP3324GMY1b1) captured on COOH chip can bind Human ACE2 protein Fc tag (CSB-MP866317HU) with an affinity constant of 13.8 nM as detected by LSPR Assay.
||ACE 2; ACE related carboxypeptidase; ACE-related carboxypeptidase; ACE2; ACE2_HUMAN; ACEH; Angiotensin converting enzyme 2; Angiotensin converting enzyme homolog; Angiotensin converting enzyme like protein; Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2; Angiotensin I converting enzyme 2; Angiotensin-converting enzyme homolog; DKFZP434A014; EC 3.4.17; metalloprotease MPROT 15; Metalloprotease MPROT15; OTTHUMP00000022963; Processed angiotensin-converting enzyme 2
||Homo sapiens (Human)
|Target Protein Sequence
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
|| Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
||Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
||3-7 business days
||Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
|Datasheet & COA
|| Please contact us to get it.