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The recombinant human ACE2 is prepared by the expression of a target fragment corresponding to 18-740aa of the human ACE2 protein with a human Fc tag at the C-terminus in the mammalian expression system.
The monocarboxypeptidase ACE2, a homolog of ACE, is a negative regulator of the renin-angiotensin-aldosterone system (RAAS). It plays a protective role in the cardiovascular system and many organs by balancing multiple functions of ACE. And ACE2 is also the primary target for SARS-CoV-2 and facilitates viral invasion into the host cells. Binding with an amino acid transporter, ACE2 is conducive to the absorption of amino acids in the kidney and intestines.
This human ACE2 contains endotoxin less than 1.0 EU/ug as determined by the LAL method, and its purity is over 90% as measured by SDS-PAGE. It has high reactivity and binding-affinity with SARS-CoV-2-S1 and SARS-CoV-2-S1-RBD, which were verified in the functional ELISA and LSPR assay, respectively. As is well-known, a recombinant protein tagged with Fc is relatively more stable & productive and efficient to be purified by protein A. And this ACE2 protein carries a hFc-tag, which can bind to the Fc receptor on the surface of immune cells, exerting multiple biological effects.
Applications : Surface plasmon resonance (SPR)
Review: Using real-time biomolecular interaction analysis with surface plasmon resonance (SPR), we further studied the interaction of HDP-2P with ACE2 protein. The results demonstrated that HDP-2P could bind to the ACE2.
Applications : Antigen
Review: I used CSB-MP866317HU protein and conducted ELISA experiments with antibodies from different manufacturers and batches, and all of them have good fitting curves, high protein activity, and excellent repeatability. The subsequent experiment progressed smoothly and saved a lot of time for me! I will continue to purchase in large quantities!
What QC test will you perform?
When making ACE2, do you use any protein denaturing purification steps?
I would like to know if this protein has information on tests or information that can verify that there are no differences between this recombinant protein and the protein in its physiological state.
Has this protein been verified for its activity?
Do you have ACE2 protein validated with the spike protein?
What's the difference between CSB-MP866317HU and CSB-AP005671HU?
Can you remove the endotoxin?
What is the general preservative? Which kind of preservative do you usually add?
What is the general protectant? What kind of protectant do you usually add?
Can you please respond to below mentioned queries:
1) The source is Mammalian cell. It is human origin or some other mammalian cell?
2) The protein is available in lyophilized form or liquid?
3) If it is in lyophilized form, can you please provide reconstitution instructions?