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| Code | CSB-RA987582A0HU |
| Size | US$350 |
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| Have Questions? | Leave a Message or Start an on-line Chat |
| Description |
SAE1 is an E-activating enzyme that plays a vital role in SUMOylation, a post-translational modification involved in cellular events such as regulation of transcription, cell cycle, and apoptosis. SAE1 forms a thioester bond between SUMO protein and UBA2/SAE2 by regulating the activation of ATP-dependent SUMO protein and then participates in the process of SUMO protein modification. SAE1 mainly mediates acetylation and phosphorylation and plays an important role in chromosome division and apoptosis. Upregulated expression of SAE1 has been implicated in the tumorigenesis and progression of several human malignancies, including glioma and gastric cancer. The main steps in the production of this SAE1 recombinant antibody include immunization; harvest of positive spleen cells; obtaining the antibody sequence by screening and sequencing; expression of the target antibody in mammalian cells; purification. The SAE1 antibody was produced recombinantly and has many advantages: high reproducibility, specificity and scalability. And has been validated in ELISA, WB, IF. |
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| Uniprot No. | Q9UBE0 | ||||||
| Target Names | SAE1 | ||||||
| Alternative Names |
SUMO-activating enzyme subunit 1 (Ubiquitin-like 1-activating enzyme E1A) [Cleaved into: SUMO-activating enzyme subunit 1, N-terminally processed], SAE1, AOS1 SUA1 UBLE1A
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| Species Reactivity | Human | ||||||
| Immunogen | A synthesized peptide derived from human SAE1 | ||||||
| Immunogen Species | Homo sapiens (Human) | ||||||
| Conjugate | Non-conjugated | ||||||
| Clonality | Monoclonal | ||||||
| Isotype | Rabbit IgG | ||||||
| Clone No. | 9D8 | ||||||
| Purification Method | Affinity-chromatography | ||||||
| Concentration | It differs from different batches. Please contact us to confirm it. | ||||||
| Buffer | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | ||||||
| Form | Liquid | ||||||
| Tested Applications | ELISA, WB, IF | ||||||
| Recommended Dilution |
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| Protocols | ELISA Protocol Western Blotting(WB) Protocol Immunofluorescence (IF) Protocol |
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| Troubleshooting and FAQs | Antibody FAQs | ||||||
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | ||||||
| Lead Time | Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
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| Function |
The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
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| Gene References into Functions |
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| Subcellular Location | Nucleus. |
| Protein Families | Ubiquitin-activating E1 family |
| Tissue Specificity | Expression level increases during S phase and drops in G2 phase (at protein level). |
| Database Links |
HGNC: 30660 OMIM: 613294 KEGG: hsa:10055 STRING: 9606.ENSP00000270225 UniGene: Hs.515500 |
