SAE1 Recombinant Monoclonal Antibody

Code CSB-RA987582A0HU
Size US$210
Order now
Image
  • Western Blot
    Positive WB detected in: K562 whole cell lysate
    All lanes: SAE1 Antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 39, 30, 34 kDa
    Observed band size: 39 kDa
  • Immunofluorescence staining of Hela Cells with CSB-RA987582A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Uniprot No.
Target Names
SAE1
Alternative Names
SUMO-activating enzyme subunit 1 (Ubiquitin-like 1-activating enzyme E1A) [Cleaved into: SUMO-activating enzyme subunit 1, N-terminally processed], SAE1, AOS1 SUA1 UBLE1A
Species Reactivity
Human
Immunogen
A synthesized peptide derived from human SAE1
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
9D8
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IF 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The process of producing the SAE1 recombinant antibody involves four main steps. Firstly, the SAE1 monoclonal antibody gene is sequenced. Next, the gene is cloned into a plasmid vector. After that, the recombinant vector is introduced into a host cell line. Then, the SAE1 recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography. Finally, the purified antibody is tested and characterized. The SAE1 monoclonal antibody is developed from the SAE1 antibody-producing hybridomas and a synthesized peptide derived from human SAE1 is used as the immunogen during its production. This SAE1 recombinant monoclonal antibody is recommended for use in ELISA, WB, and IF applications to detect human SAE1 protein.

The SAE1 protein plays a crucial role in the process of protein sumoylation, which can alter protein function, stability, localization, and interactions with other proteins. The SAE1 protein, along with its binding partner SAE2, forms the heterodimeric SUMO-activating enzyme (SAE) complex, which is responsible for activating SUMO proteins by catalyzing the formation of a thioester bond between the C-terminus of SUMO and a cysteine residue on SAE1. This activates the SUMO protein, allowing it to be transferred to a target protein by SUMO-conjugating enzymes, resulting in the sumoylation of the target protein. It also participates in regulating various cellular processes, including DNA repair, transcriptional regulation, and protein localization. Dysregulation of sumoylation has been associated with several human diseases, including cancer, neurodegenerative disorders, and viral infections, highlighting the importance of SAE1 in maintaining cellular homeostasis.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Gene References into Functions
  1. the expression of SENP8, SAE1, PIAS1, PIAS2 and ZMIZ1 is deregulated in the majority of PTC tissues, likely contributing to the PTC phenotype. PMID: 26403403
  2. as it has been shown by flow cytometry analysis, specific knockdown of SAE1 slowed down the cell population at G0/G1 phase and induced apoptosis of RKO cells. PMID: 25842831
  3. SUMO-1 controls the protein stability and the biological function of phosducin. PMID: 16421094
Subcellular Location
Nucleus.
Protein Families
Ubiquitin-activating E1 family
Tissue Specificity
Expression level increases during S phase and drops in G2 phase (at protein level).
Database Links

HGNC: 30660

OMIM: 613294

KEGG: hsa:10055

STRING: 9606.ENSP00000270225

UniGene: Hs.515500

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
webinars: DT3C facilitates antibody internalization X
Place an order now

I. Product details

*
*
*
*

II. Contact details

*
*

III. Ship To

*
*
*
*
*
*
*

IV. Bill To

*
*
*
*
*
*
*
*