SAE1 Antibody

Code CSB-RA987582A0HU
Size US$350
  • Western Blot
    Positive WB detected in: K562 whole cell lysate
    All lanes: SAE1 Antibody at 1:1000
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 39, 30, 34 kDa
    Observed band size: 39 kDa
  • Immunofluorescence staining of Hela Cells with CSB-RA987582A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Product Details


SAE1 is an E-activating enzyme that plays a vital role in SUMOylation, a post-translational modification involved in cellular events such as regulation of transcription, cell cycle, and apoptosis. SAE1 forms a thioester bond between SUMO protein and UBA2/SAE2 by regulating the activation of ATP-dependent SUMO protein and then participates in the process of SUMO protein modification. SAE1 mainly mediates acetylation and phosphorylation and plays an important role in chromosome division and apoptosis. Upregulated expression of SAE1 has been implicated in the tumorigenesis and progression of several human malignancies, including glioma and gastric cancer.

The main steps in the production of this SAE1 recombinant antibody include immunization; harvest of positive spleen cells; obtaining the antibody sequence by screening and sequencing; expression of the target antibody in mammalian cells; purification. The SAE1 antibody was produced recombinantly and has many advantages: high reproducibility, specificity and scalability. And has been validated in ELISA, WB, IF.

Uniprot No. Q9UBE0
Target Names SAE1
Alternative Names
SUMO-activating enzyme subunit 1 (Ubiquitin-like 1-activating enzyme E1A) [Cleaved into: SUMO-activating enzyme subunit 1, N-terminally processed], SAE1, AOS1 SUA1 UBLE1A
Species Reactivity Human
Immunogen A synthesized peptide derived from human SAE1
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype Rabbit IgG
Clone No. 9D8
Purification Method Affinity-chromatography
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Tested Applications ELISA, WB, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IF 1:20-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Gene References into Functions
  1. the expression of SENP8, SAE1, PIAS1, PIAS2 and ZMIZ1 is deregulated in the majority of PTC tissues, likely contributing to the PTC phenotype. PMID: 26403403
  2. as it has been shown by flow cytometry analysis, specific knockdown of SAE1 slowed down the cell population at G0/G1 phase and induced apoptosis of RKO cells. PMID: 25842831
  3. SUMO-1 controls the protein stability and the biological function of phosducin. PMID: 16421094
Subcellular Location Nucleus.
Protein Families Ubiquitin-activating E1 family
Tissue Specificity Expression level increases during S phase and drops in G2 phase (at protein level).
Database Links

HGNC: 30660

OMIM: 613294

KEGG: hsa:10055

STRING: 9606.ENSP00000270225

UniGene: Hs.515500

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