Code | CSB-RA249987A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:200 |
In efforts to develop a recombinant monoclonal antibody specific to SATB2, the initial step involved the immunization of a rabbit with a synthesized peptide derived from human SATB2 protein. Following immunization, B cells were isolated from the rabbit, and RNA was extracted from these cells. The extracted RNA was reverse-transcribed into cDNA, which served as a template for extending SATB2 antibody genes using degenerate primers. These engineered SATB2 antibody genes were then integrated into a plasmid vector and introduced into host cells for expression. Subsequently, the SATB2 recombinant monoclonal antibody was isolated from the cell culture supernatant via affinity chromatography and assessed for its suitability in ELISA and IHC applications. It only shows reactivity with human SATB2 protein.
SATB2 is a multifunctional protein that plays crucial roles in development, cell differentiation, and gene regulation. Its functions are particularly important in craniofacial development, bone formation, neuronal development, and the maintenance of chromatin structure. Dysregulation of SATB2 can have significant implications for health and development.
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