SNTA1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA047353A0HU
Size	US$210
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Image	IHC image of CSB-RA047353A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of HepG2 cell with CSB-RA047353A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing PC-3 cells stained with CSB-RA047353A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q13424

Target Names

SNTA1

Alternative Names

Alpha-1-syntrophin (59 kDa dystrophin-associated protein A1 acidic component 1) (Pro-TGF-alpha cytoplasmic domain-interacting protein 1) (TACIP1) (Syntrophin-1), SNTA1, SNT1

Species Reactivity

Human

Immunogen

A synthesized peptide from human SNTA1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

1F3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SNTA1, also known as alpha-1-syntrophin, serves as a critical scaffolding protein within the dystrophin-associated protein complex, where it anchors signaling molecules to the plasma membrane in muscle and neuronal tissues. This adapter protein plays essential roles in maintaining membrane integrity, modulating ion channel localization, and facilitating signal transduction pathways. Its involvement in muscular dystrophies and cardiac function makes SNTA1 a significant target for researchers investigating neuromuscular disorders, cardiac pathophysiology, and membrane-associated signaling networks.

This recombinant monoclonal antibody, clone 1F3, offers the reproducibility and consistency that demanding research applications require. Generated against a synthetic peptide derived from human SNTA1, the antibody is produced using recombinant technology, ensuring sequence-defined specificity and eliminating the lot-to-lot variability often encountered with traditional hybridoma-derived reagents. The rabbit IgG format provides excellent signal amplification while the affinity-chromatography purification delivers a clean reagent suitable for sensitive detection methods.

Validation across multiple platforms demonstrates this antibody's versatility in your experimental workflows. Immunohistochemistry studies in paraffin-embedded human cervical cancer tissue reveal clear target detection using citrate buffer antigen retrieval at dilutions between 1:50 and 1:200. Immunofluorescence analysis in HepG2 hepatocellular carcinoma cells shows distinct cellular localization patterns, while flow cytometry experiments using PC-3 prostate cancer cells confirm reliable detection in suspension-based assays with appropriate signal separation from isotype controls.

Whether you are investigating dystrophin complex assembly, exploring SNTA1's role as a TGF-alpha cytoplasmic domain-interacting protein, or examining its function in disease models, this antibody provides a dependable tool for advancing your understanding of membrane-associated scaffolding proteins.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Adapter protein that binds to and probably organizes the subcellular localization of a variety of membrane proteins. May link various receptors to the actin cytoskeleton and the extracellular matrix via the dystrophin glycoprotein complex. Plays an important role in synapse formation and in the organization of UTRN and acetylcholine receptors at the neuromuscular synapse. Binds to phosphatidylinositol 4,5-bisphosphate.

Gene References into Functions

not associated with sudden infant death syndrome PMID: 28520217
Low SNTA expression is associated with non-alcoholic steatohepatitis but is unchanged in hepatocellular carcinoma. PMID: 28941732
A novel SNTA1 variant is likely causative for drug induced long-QT syndrome by augmenting the late sodium current. PMID: 27028743
In a nonreferred nationwide Danish cohort of SIDS cases, up to 5/66 (7.5%) of SIDS cases can be explained by genetic variants in the sodium channel complex genes. PMID: 25757662
our results present a possible mechanism of Rac1 activation involving SNTA1 and emphasise its role in ROS generation, cell migration, and acquisition of malignancy. PMID: 24434436
Ordered disorder of the astrocytic dystrophin-associated protein complex in the norm and pathology. PMID: 24014171
alpha-Syntrophin, which resides in nuclei of myocytes, functions as the upstream mediator of nuclear nNOS translocation and nNOS-dependent mitochondrial biogenesis. PMID: 24235139
Calcium homeostasis mishandling in Duchenne muscular dystrophy myotubes depends on store operated calcium entry under the influence alpha1-syntrophin regulation as well as TRPV2-dependant cation influx. PMID: 23426965
The combined mutations of A261V-SNTA1 plus R800L-SCN5A increase the INa current late/peak ratio and time constants of current decay. PMID: 23376825
In contrast to stomach, lung, colon and rectal cancers, SNTA1 protein was found to be downregulated in esophageal cancers and upregulated in breast cancer. PMID: 21091386
alpha1D-adrenergic receptors are regulated by syntrophins through a PDZ domain-mediated interaction PMID: 16533813
These results establish an SNTA1-based nNOS complex attached to SCN5A as a key regulator of sodium current and suggest that SNTA1 be considered a rare long QT syndrome-susceptibility gene. PMID: 18591664
SNTA1 is a new susceptibility gene for LQTS. A257G-SNTA1 can cause gain-of-function of Na(v)1.5 similar to the LQT3. PMID: 19684871
Alpha1-syntrophin mutations identified in sudden infant death syndrome cause an increase in late cardiac sodium current. PMID: 20009079

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Involvement in disease

Long QT syndrome 12 (LQT12)

Subcellular Location

Cell membrane, sarcolemma; Peripheral membrane protein; Cytoplasmic side. Cell junction. Cytoplasm, cytoskeleton.

Protein Families

Syntrophin family

Tissue Specificity

High expression in skeletal muscle and heart. Low expression in brain, pancreas, liver, kidney and lung. Not detected in placenta.

Database Links

HGNC: 11167

OMIM: 601017

KEGG: hsa:6640

STRING: 9606.ENSP00000217381

UniGene: Hs.31121

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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