SOCS7 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA594821A0HU
Size	US$210
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Image	IHC image of CSB-RA594821A0HU diluted at 1:50 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.43% DAB. Overlay Peak curve showing HepG2 cells stained with CSB-RA594821A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

O14512

Target Names

SOCS7

Alternative Names

Suppressor of cytokine signaling 7 (SOCS-7) (Nck, Ash and phospholipase C gamma-binding protein) (Nck-associated protein 4) (NAP-4), SOCS7, NAP4 SOCS6

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human SOCS7

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

23E4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The SOCS7 recombinant monoclonal antibody is generated via in vitro cloning, involving the integration of genes encoding both SOCS7 antibody's heavy and light chains into expression vectors. These vectors are then introduced into host cells to facilitate the recombinant antibody's expression within a cell culture context. Following expression, the SOCS7 recombinant monoclonal antibody is purified from the supernatant of transfected host cell lines, utilizing an affinity-chromatography-based purification method. Notably, this antibody exhibits high specificity in binding to the human SOCS7 protein and is remarkably versatile and suitable for three applications, including ELISA, IHC, and FC.

SOCS7 is a negative regulator of cytokine and growth factor signaling pathways, primarily through its involvement in the JAK-STAT pathway. Its function is to prevent excessive immune responses, regulate cell growth and differentiation, and maintain homeostasis. Dysregulation of SOCS7 can have implications for various diseases and conditions.

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Target Background

Function

Regulates signaling cascades probably through protein ubiquitination and/or sequestration. Functions in insulin signaling and glucose homeostasis through IRS1 ubiquitination and subsequent proteasomal degradation. Inhibits also prolactin, growth hormone and leptin signaling by preventing STAT3 and STAT5 activation, sequestering them in the cytoplasm and reducing their binding to DNA. May be a substrate recognition component of a SCF-like E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

Gene References into Functions

This study showed that increased protein levels of SOCS-4 and SOCS-7 in Alzheimer's disease brains. PMID: 25286386
This SOCS7 knockdown-attributed effect could be due to a precise anti-PLCg-1 role. PMID: 25162020
genetic variants in the SOCS7 gene do not impact variation in glucose homeostasis traits and only minimally impact risk of T2DM in the Old Order Amish PMID: 23767996
Insulin Like Growth Factor I treatment and SOCS7 loss have synergistically resulted in increased growth and migration of MCF7 and in increased migration of MDA-MB-231 cells. PMID: 24046004
The present report describes for the first time associations between common variants in SOCS7 gene and obesity, central obesity, insulin resistance and disorders of lipid metabolism in nondiabetic men from Argentina. PMID: 22397880
SOCS7 functioned as an oncogene, the finding that revealed a novel mechanism of carcinogenesis in bladder cancer cells. PMID: 23392170
LNCaP-S17 cells are resistant to exogenous IL-6-induced neuroendocrine differentiation due to increased levels of CIS/SOCS7 that block activation of JAK2-STAT3 pathways. PMID: 22213096
Higher mRNA expression levels of SOCS1, 3, 4 and 7 are significantly associated with earlier tumour stage and better clinical outcome in human breast cancer. PMID: 20433750
findings show that PPAR-gamma is involved in the regulation of SOCS-7 expression by hepatitis C virus core protein genotype 3a PMID: 20357037
Taken together, our data indicate that SOCS-7 interacts with vinexin and the actin cytoskeleton. PMID: 15242778
Results indicate that SOCS-7 is a dysregulator of prolactin, leptin, and growth hormone signaling and that its mode of action is a variation of SOCS protein inhibition of cytokine-inducible STAT3 and 5-mediated signal transduction. PMID: 15677474
data suggest that SOCS7 is a potent regulator of glucose homeostasis and insulin signaling PMID: 16127460

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Subcellular Location

Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus. Note=Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Rapidly relocalizes to the nucleus after UV irradiation. Cytoplasmic location depends upon SEPT7 presence.

Tissue Specificity

Expressed in brain and leukocytes. Also in fetal lung fibroblasts and fetal brain.

Database Links

HGNC: 29846

OMIM: 608788

KEGG: hsa:30837

STRING: 9606.ENSP00000464034

UniGene: Hs.514132

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Jak-STAT signaling pathway

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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