SOCS7 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA594821A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA594821A0HU diluted at 1:50 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.43% DAB. Overlay Peak curve showing HepG2 cells stained with CSB-RA594821A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

O14512

Target Names

SOCS7

Alternative Names

Suppressor of cytokine signaling 7 (SOCS-7) (Nck, Ash and phospholipase C gamma-binding protein) (Nck-associated protein 4) (NAP-4), SOCS7, NAP4 SOCS6

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human SOCS7

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

23E4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SOCS7 plays a critical role in the negative regulation of cytokine and growth factor signaling pathways, functioning as part of the suppressor of cytokine signaling family that modulates JAK-STAT pathway activity. Also known as Nck-associated protein 4, this protein participates in complex signaling networks involving Nck, Ash, and phospholipase C gamma, making it a valuable target for researchers investigating signal transduction mechanisms, metabolic regulation, and immune response modulation.

This recombinant monoclonal antibody, clone 23E4, offers the consistency and reliability that demanding experimental workflows require. Produced using recombinant technology with a defined sequence, this rabbit IgG antibody eliminates the lot-to-lot variability that can compromise longitudinal studies and reproducibility. The antibody was raised against a synthetic peptide derived from human SOCS7 and purified by affinity chromatography to ensure high specificity for your target.

Validation studies demonstrate robust performance across multiple applications. Immunohistochemistry testing in paraffin-embedded human testis tissue using a Leica Bond system with citrate buffer antigen retrieval shows clear specific staining at dilutions between 1:50 and 1:200. Flow cytometry analysis in HepG2 cells confirms intracellular detection capability, with permeabilized cells showing a distinct positive shift compared to isotype controls when used at 1:50 dilution. The antibody is also validated for ELISA applications.

Supplied in a glycerol-containing buffer optimized for long-term stability at -20°C or -80°C, this unconjugated antibody provides flexibility for detection system selection. Whether you are mapping SOCS7 expression patterns in human tissues or quantifying protein levels in hepatocellular models, this antibody supports rigorous investigation of cytokine signaling regulation.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Regulates signaling cascades probably through protein ubiquitination and/or sequestration. Functions in insulin signaling and glucose homeostasis through IRS1 ubiquitination and subsequent proteasomal degradation. Inhibits also prolactin, growth hormone and leptin signaling by preventing STAT3 and STAT5 activation, sequestering them in the cytoplasm and reducing their binding to DNA. May be a substrate recognition component of a SCF-like E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

Gene References into Functions

This study showed that increased protein levels of SOCS-4 and SOCS-7 in Alzheimer's disease brains. PMID: 25286386
This SOCS7 knockdown-attributed effect could be due to a precise anti-PLCg-1 role. PMID: 25162020
genetic variants in the SOCS7 gene do not impact variation in glucose homeostasis traits and only minimally impact risk of T2DM in the Old Order Amish PMID: 23767996
Insulin Like Growth Factor I treatment and SOCS7 loss have synergistically resulted in increased growth and migration of MCF7 and in increased migration of MDA-MB-231 cells. PMID: 24046004
The present report describes for the first time associations between common variants in SOCS7 gene and obesity, central obesity, insulin resistance and disorders of lipid metabolism in nondiabetic men from Argentina. PMID: 22397880
SOCS7 functioned as an oncogene, the finding that revealed a novel mechanism of carcinogenesis in bladder cancer cells. PMID: 23392170
LNCaP-S17 cells are resistant to exogenous IL-6-induced neuroendocrine differentiation due to increased levels of CIS/SOCS7 that block activation of JAK2-STAT3 pathways. PMID: 22213096
Higher mRNA expression levels of SOCS1, 3, 4 and 7 are significantly associated with earlier tumour stage and better clinical outcome in human breast cancer. PMID: 20433750
findings show that PPAR-gamma is involved in the regulation of SOCS-7 expression by hepatitis C virus core protein genotype 3a PMID: 20357037
Taken together, our data indicate that SOCS-7 interacts with vinexin and the actin cytoskeleton. PMID: 15242778
Results indicate that SOCS-7 is a dysregulator of prolactin, leptin, and growth hormone signaling and that its mode of action is a variation of SOCS protein inhibition of cytokine-inducible STAT3 and 5-mediated signal transduction. PMID: 15677474
data suggest that SOCS7 is a potent regulator of glucose homeostasis and insulin signaling PMID: 16127460

Hide All

Subcellular Location

Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus. Note=Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Rapidly relocalizes to the nucleus after UV irradiation. Cytoplasmic location depends upon SEPT7 presence.

Tissue Specificity

Expressed in brain and leukocytes. Also in fetal lung fibroblasts and fetal brain.

Database Links

HGNC: 29846

OMIM: 608788

KEGG: hsa:30837

STRING: 9606.ENSP00000464034

UniGene: Hs.514132

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Jak-STAT signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address