ST13 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA116588A0HU
Size	US$210
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Image	IHC image of CSB-RA116588A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA116588A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing A431 cells stained with CSB-RA116588A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

P50502

Target Names

ST13

Alternative Names

Hsc70-interacting protein (Hip) (Aging-associated protein 2) (Progesterone receptor-associated p48 protein) (Protein FAM10A1) (Putative tumor suppressor ST13) (Renal carcinoma antigen NY-REN-33) (Suppression of tumorigenicity 13 protein), ST13, AAG2 FAM10A1 HIP SNC6

Species Reactivity

Human

Immunogen

A synthesized peptide from human ST13 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

7F12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

ST13, also known as Hsc70-interacting protein (Hip), functions as a co-chaperone that stabilizes the ADP-bound form of Hsc70, playing a critical role in protein folding and cellular stress responses. Originally identified as a putative tumor suppressor, ST13 has garnered significant research interest due to its involvement in cancer biology, with altered expression patterns observed across multiple malignancies. Its alternative designations as a progesterone receptor-associated protein and renal carcinoma antigen further underscore its relevance to hormone signaling and oncology research.

This recombinant monoclonal antibody, clone 7F12, offers the reproducibility essential for longitudinal studies and multi-site collaborations. Generated against a synthetic peptide derived from human ST13, the antibody is produced using recombinant technology that ensures sequence-defined consistency between lots, eliminating the variability that can compromise experimental reproducibility when working with traditional hybridoma-derived reagents.

Validation studies demonstrate reliable performance across multiple applications. Immunohistochemistry testing in paraffin-embedded human tissues reveals clear cytoplasmic staining in both breast cancer and pancreatic tissue sections at 1:100 dilution using citrate buffer antigen retrieval, providing researchers with optimized starting conditions for their own tissue studies. Flow cytometry analysis using A431 epidermoid carcinoma cells shows a distinct positive shift compared to isotype controls, confirming the antibody's utility for intracellular protein detection in fixed and permeabilized cells.

The unconjugated format and affinity-purified preparation provide flexibility for researchers to pair this antibody with their preferred detection systems. Whether investigating ST13's role in chaperone-mediated protein quality control, exploring its tumor suppressor functions, or examining its expression patterns in clinical specimens, this antibody delivers the consistency and validated performance that rigorous research demands.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

One HIP oligomer binds the ATPase domains of at least two HSC70 molecules dependent on activation of the HSC70 ATPase by HSP40. Stabilizes the ADP state of HSC70 that has a high affinity for substrate protein. Through its own chaperone activity, it may contribute to the interaction of HSC70 with various target proteins.

Gene References into Functions

A novel susceptibility gene, ST13, coding for a cochaperone of the glucocorticoid receptor, is associated with exacerbations in asthmatic children and young adults despite their ICS use. PMID: 25616159
St13 is a proliferation regulator that inhibits tumor growth in colorectal neoplasms and may affect cell migration. PMID: 23125081
Akt phosphorylation at Thr308 and Ser473 is required for CHIP-mediated ubiquitination of the kinase. PMID: 21767636
A single amino acid substitution (L211S) resulted in a loss of Hip function. This mutation also appears to disrupt the interaction of Hip with Hsp70 in vitro PMID: 21240662
Hip has been identified as a novel substrate of GRK5 in vitro and in cells, and phosphorylation of Hip by GRK5 plays a role in modulating CXCR4 internalization PMID: 21728385
These results strongly indicate ST13 mediated by adenovirus triggers colorectal cell apoptosis via ASK1-JNK signaling cascade PMID: 20512919
S-nitrosoglutathione corrects DeltaF508 CFTR trafficking by inhibiting Hop expression PMID: 20534503
The expression levels of ST13 cannot serve as a biomarker for early stages of PD. PMID: 20193743
Hip functions to promote the efficiency of glucocorticoid receptor maturation in cells PMID: 15071092
The expression of ST13 decreases in colorectal cancer tissue compared with that in adjacent normal tissue. ST13 is mostly expressed in colorectal epithelia and adenocarcinoma cells. PMID: 15637739
tetratricopeptide repeat domain is required for effects of CHIP on alpha-synuclein inclusion morphology, number of inclusions, and proteasomal degradation as well as the direct interaction of CHIP with Hsp70 implicates their cooperation in these processes PMID: 15845543
The expression levels of the ST13 gene were significantly decreased in primary tumors compared with adjacent mucosa (P<0.05). PMID: 16358374
results suggest that granzyme B-mediated loss of HIP expression occurs in vivo, and in a coordinate fashion with loss of BID, pro-caspase-8 and pro-caspase-3; data implicate the Hsp70 co-chaperone HIP in the proteolytic cascade of some apoptotic pathways PMID: 17013759
analysis of similarity between the chloroplast translocon component, Tic40, and the human co-chaperone, Hsp70-interacting protein PMID: 17535810

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Subcellular Location

Cytoplasm.

Protein Families

FAM10 family

Database Links

HGNC: 11343

OMIM: 606796

KEGG: hsa:6767

STRING: 9606.ENSP00000216218

UniGene: Hs.712713

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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