STAG2 Recombinant Monoclonal Antibody

Code CSB-RA292183A0HU
Size US$210
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  • Western Blot
    Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate
    All lanes: SA2 antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 142, 146 kDa
    Observed band size: 142 kDa
  • Immunofluorescence staining of MCF7 Cells with CSB-RA292183A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Product Details

Uniprot No.
Target Names
STAG2
Alternative Names
Cohesin subunit SA-2 (SCC3 homolog 2) (Stromal antigen 2), STAG2, SA2
Species Reactivity
Human
Immunogen
A synthesized peptide derived from human SA2
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
3H4
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IF 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

STAG2 encodes a subunit of the cohesin complex and is one of the most mutated genes in human cancer. STAG2 inactivation has been demonstrated to cause aneuploidy via sister chromatid cohesion, as well as increased DNA damage, which could facilitate further mutagenesis. STAG2 is required for the progression of DNA replication forks. STAG2 deficiency disrupts the interaction of cohesin with the replication machinery, resulting in replication fork stalling and collapse, as well as failure to develop SMC3 acetylation. As a result, STAG2 loss causes synthetic lethality with particular DNA repair genes as well as increased chemotherapeutic susceptibility.

CUSABIO cloned STAG2 antibody-coding genes into plasma vectors and then transfected these vector clones into mammalian cells using a lipid-based transfection reagent. Following transient expression, the recombinant antibodies against STAG2 were harvested and characterized. The recombinant STAG2 antibody was purified by Affinity-chromatography from the culture medium. It can be used to detect STAG2 protein from Human in the ELISA, WB, IF.

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Target Background

Function
Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
Gene References into Functions
  1. STAG2 deficiency induces interferon responses via cGAS-STING pathway and restricts virus infection. PMID: 29662124
  2. STAG2 loss of Expression is Associated with Cancer Progression in Upper Urinary Tract Carcinoma. PMID: 28967037
  3. these data suggest that STAG2 acts as a tumor suppressor gene in bladder cancer and may be a potential therapeutic target in bladder cancer PMID: 28627627
  4. Extending the lifespan of normal human cells due to inactivation of STAG2 could promote tumorigenesis by extending the period during which tumor-driving mutations occur. PMID: 28819029
  5. TAG2 is the most commonly mutated subunit, and in a recent analysis was identified as one of only 12 genes that are significantly mutated in four or more cancer types. PMID: 27207471
  6. the clinical features of these three cases are remarkably similar to those observed in other well-established cohesinopathies. Herein, we suggest that STAG2 is a dosage-sensitive gene and that heterozygous loss-of-function variants lead to a cohesinopathy. PMID: 28296084
  7. results indicated that the complete loss of STAG2 expression was predictive for better recurrence-free survival and cancer-specific survival, suggesting its potential value as a prognostic biomarker in bladder cancer PMID: 26838030
  8. We suggest that increased STAG2 gene copy number and dysregulation of its downstream target genes may be responsible for the specific clinical findings of this syndrome. PMID: 25677961
  9. Characterization of C-terminal nuclear localization signal of the human SA2 stromalin PMID: 25979289
  10. Data show a significantly higher stromal antigen 2 (STAG2) mRNA and protein levels in normal bladder cells than bladder cancer cells. PMID: 25867412
  11. Microduplication of chromosome Xq25 encompassing STAG2 gene in a boy with intellectual disability PMID: 25450604
  12. STAG2 promotes the correction of kMT attachment errors to ensure faithful chromosome segregation during mitosis. PMID: 25074805
  13. Genomic landscape of Ewing sarcoma defines an aggressive subtype with co-association of STAG2 and TP53 mutations PMID: 25223734
  14. Loss of STAG2 expression occurs in 15% of tumors and is associated with metastatic disease, suggesting a potential genetic vulnerability in Ewing sarcoma PMID: 25186949
  15. our study identifies the duplication of XIAP and STAG2 as the minimal duplicated region leading to the ID, facial morphological anomalies, and speech delay, specific to the patients with Xq25 duplication. PMID: 24733578
  16. In an independent EFT tissue microarray cohort, we show that STAG2 loss as detected by immunohistochemistry may be associated with more advanced disease (p = 0.15) and a modest decrease in overall survival (p = 0.10). PMID: 25010205
  17. Aneuploidy in human salivary gland carcinomas is not driven by loss of expression of STAG2. PMID: 24822266
  18. These data suggest that PARP is a potential target for tumors harboring inactivating mutations in STAG2, and strongly recommend that STAG2 status be determined and correlated with therapeutic response to PARP inhibitors PMID: 24356817
  19. Cross-sectional deep-sequencing analysis for clonal hierarchy demonstrated STAG2, SMC3, and RAD21 mutations to be ancestral in 18%, 18%, and 47% of cases, respectively, and each expanded to clonal dominance concordant with disease transformation PMID: 25006131
  20. Loss of STAG2 function is associated with non-invasive bladder cancer. PMID: 24270882
  21. Mutations in STAG2 is associated with acute myeloid leukemia. PMID: 24335498
  22. STAG2 is one of the most commonly mutated genes in bladder cancer. PMID: 24121789
  23. STAG2 is a new urothelial bladder cancer tumor suppressor acting through mechanisms that are different from its role in preventing aneuploidy. PMID: 24121791
  24. Inactivating point mutations in the STAG2 gene are not common in neuroblastoma tumors PMID: 24088605
  25. Somatic mutation of STAG2, an aneuploidy-related gene, is rare in acute leukemias. PMID: 22132872
  26. Low STAG2 expression and not mutation is associated with neoplasms. PMID: 22668012
  27. study has shown that diverse human cancers harbor mutations in the X-linked chromatid cohesion gene STAG2 and that these mutations cause aneuploidy PMID: 21852505
  28. evidence suggests STAG2 functions as a transcriptional co-activator by a mechanism involving protein-protein interactions with transcription factors PMID: 14660624
  29. Phosphorylation of SA2 is essential for cohesin dissociation during prophase and prometaphase, but is not required for cohesin cleavage by separase. PMID: 15737063
  30. Cohesion between sister chromatids is essential for their bi-orientation on mitotic spindles is mediated by a multisubunit complex called cohesin. PMID: 15737064

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Subcellular Location
Nucleus. Chromosome. Chromosome, centromere. Note=Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK1, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex.
Protein Families
SCC3 family
Database Links

HGNC: 11355

OMIM: 300826

KEGG: hsa:10735

STRING: 9606.ENSP00000218089

UniGene: Hs.496710

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