STK33 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA207790A0HU
Size	US$210
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Image	IHC image of CSB-RA207790A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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Product Details
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Target Background

Product Details

Uniprot No.

Q9BYT3

Target Names

STK33

Alternative Names

Serine/threonine-protein kinase 33 (EC 2.7.11.1), STK33

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human STK33

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

5H11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The production of the STK33 recombinant monoclonal antibody follows a stringent and well-defined process to ensure its quality and specificity. Initially, B cells are isolated from an immunized animal, with the synthesized peptide derived from human STK33 used as the immunogen. Subsequently, total RNA is extracted from the isolated B cells and converted into cDNA through reverse transcription. The STK33 antibody genes are then amplified using PCR with specific primers targeting the antibody constant regions and inserted into an expression vector. This vector is introduced into host cells, facilitating the production of the STK33 recombinant monoclonal antibody. The antibody is harvested from the cell culture supernatant and purified using affinity chromatography, resulting in a highly purified form. Rigorous characterization assays, including ELISA and IHC analysis, are performed to validate the antibody's specificity and functionality, ensuring its precise recognition of human STK33 protein.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Serine/threonine protein kinase which phosphorylates VIME. May play a specific role in the dynamic behavior of the intermediate filament cytoskeleton by phosphorylation of VIME. Not essential for the survival of KRAS-dependent AML cell lines.

Gene References into Functions

Dysregulated HIF1alpha/STK33 signaling promotes pancreatic ductal adenocarcinoma (PDAC) growth and progression, this suggests that STK33 is a candidate therapeutic target to improve PDAC treatment. PMID: 29038348
STK33 is a potential oncogene and plays an important role in tumorigenesis of HSCC via regulation of numerous genes. PMID: 27414193
STK33 plays an essential role in hepatocellular proliferation and liver tumorigenesis. STK33 may bind directly to c-Myc and increase its transcriptional activity. PMID: 25398772
Results show that STK33 is a potential oncogene and a promising diagnostic marker for hypopharyngeal squamous cell carcinoma (HSCC). STK33 may promote tumorigenesis and progression of HSCC, and serve as a valuable molecular target for treatment of HSCC. PMID: 25603720
STK33 can promote cell migration and invasion and suppress p53 gene expression in the NL9980 and L9981 large cell lung cancer cell lines. In addition, this protein also promotes epithelial-mesenchymal transition. PMID: 25662617
The STK33-linked SNP rs4929949 is associated with obesity and BMI in two independent cohorts of Swedish and Greek children. PMID: 23967198
STK33 downregulation or dominant mutant overexpression had no effect on KRAS signaling or survival of cancer cells. PMID: 21742770
STK33/Stk33 expression pattern resembles those of some related members of the calcium/calmodulin dependent kinase group PMID: 16176263

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Subcellular Location

Cytoplasm, perinuclear region.

Protein Families

Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CaMK subfamily

Tissue Specificity

Highly expressed in testis, fetal lung and heart, followed by pituitary gland, kidney, interventricular septum, pancreas, heart, trachea, thyroid gland and uterus. Weak hybridization signals were observed in the following tissues: amygdala, aorta, esophag

Database Links

HGNC: 14568

OMIM: 607670

KEGG: hsa:65975

STRING: 9606.ENSP00000320754

UniGene: Hs.501833

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Target Therapy

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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