Product Details

Uniprot No.

Q12846

Target Names

STX4

Alternative Names

Syntaxin-4 (Renal carcinoma antigen NY-REN-31), STX4, STX4A

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human Syntaxin 4

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4E1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Syntaxin-4 plays a central role in membrane fusion events that underpin cellular communication and signal transduction. As a SNARE protein, STX4 facilitates the docking and fusion of transport vesicles with target membranes, making it essential for processes ranging from neurotransmitter release to glucose transporter translocation in insulin-responsive tissues. Its identification as the renal carcinoma antigen NY-REN-31 has also drawn interest from researchers investigating tumor immunology and cancer biology.

This recombinant monoclonal antibody, generated from clone 4E1, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and production occurs in a controlled recombinant system, researchers can expect uniform performance across different lots, eliminating a common source of variability that can complicate long-term studies or multi-site collaborations.

Validation studies confirm robust performance in Western blot applications, where the antibody detects a band at the predicted molecular weight of 35 kDa. Testing across a diverse panel of human cell lines, including 293, Jurkat, K562, HeLa, HL-60, A375, and MCF-7, demonstrates reliable detection in both adherent and suspension cell types spanning epithelial, hematopoietic, and melanoma origins. This breadth of validated samples provides confidence when working with varied experimental models. The recommended working dilution range of 1:500 to 1:5000 for Western blot offers flexibility to optimize signal intensity based on your specific sample conditions.

Whether investigating SNARE-mediated vesicle trafficking, insulin signaling pathways, or neuronal communication mechanisms, this antibody provides a dependable tool for advancing your research into STX4 biology.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. In neurons, recruited at neurite tips to membrane domains rich in the phospholipid 1-oleoyl-2-palmitoyl-PC (OPPC) which promotes neurite tip surface expression of the dopamine transporter SLC6A3/DAT by facilitating fusion of SLC6A3-containing transport vesicles with the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes and in docking of synaptic vesicles at presynaptic active zones.

Gene References into Functions

these results indicate that the activation of beta-ARs induces secretory granules and cell membrane fusion via the interaction of VAMP-2 and syntaxin-4 in a PKA- and F-actin-dependent manner in human submandibular gland. Up-regulated beta-ARs might participate in altering protein secretion in transplanted submandibular gland by promoting the interaction of VAMP-2 with syntaxin-4. PMID: 29358308
Confocal immunofluorescence microscopy revealed a colocalization of syntaxin 4 with a MVB-specific marker, exosomes and hepatitis C virus (HCV) core, which suggests a fraction of syntaxin 4 is associated with exosomes loaded with HCV. Altogether, it is assumed that syntaxin 4 is a novel essential cellular factor for the release of HCV. PMID: 28624237
The authors found that activation of dendritic cells by bacterial lipopolysaccharide leads to increased Forster resonance energy transfer-fluorescence of fluorescently labeled syntaxin 4 with VAMP3 specifically at the plasma membrane, indicating increased SNARE complex formation, whereas FRET with other tested SNAREs was unaltered. PMID: 28524818
Data suggest MUNC18C is required for STX4-mediated invadopodium formation and tumor invasion of extracellular matrix; N-terminal STX4 fragment binds to MUNC18C and inhibits interactions of STX4 with synaptosome-associated protein 23 (SNAP23) and vesicle-associated membrane protein 2 (VAMP2). Fibrosarcoma/adenocarcinoma cell lines were used in these studies. (MUNC18C = syntaxin binding protein MUNC18C; STX4 = syntaxin 4) PMID: 28798239
The analysis revealed three candidate genes GSK3B, PTPN1, STX4 that are differentially expressed in study subjects. GSK3B was highly significant in Ps-T2D (P=0.00018, FR=-26.6), followed by Ps (P=0.0028, FR=-14.5) and T2D groups (P=0.032, FR=-5.9). PTPN1 showed significant association only with PS-T2D (P=0.00027, FR=-8.5). STX4 showed significant association with both Ps (P=0.0002, FR=-20) and Ps-T2D (P=0.0016, FR=-11.2). PMID: 27530212
When the expression of STX4 mRNA was inhibited with short or small interfering, or silencing, RNA in macrophages, the survival of Brucella melitensis was significantly reduced. PMID: 25673907
Syntaxin-4 has a role in mediating exocytosis of pre-docked and newcomer insulin granules underlying biphasic glucose-stimulated insulin secretion in human pancreatic beta cells PMID: 25762204
Increased level of SNAP23-Syntaxin4-VAMP7 interaction correlates with decreased Syntaxin4 phosphorylation and trafficking of MT1-MMP to invadopodia during cellular invasion. PMID: 24807903
upregulation of Syntaxin 4 is sufficient to significantly improve insulin secretory function to human type 2 diabetes islets retaining low levels of residual function PMID: 24552216
STX4 is implicated in the antibody secretion. PMID: 24146186
Syntaxin-4 plays a vital role in exocytosis of IgE from plasma cells. Knock-down of syntaxin-4, but not VAMP3 dramatically reduced IgE secretion from U266 plasma cells causing it to accumulate in the cell. PMID: 24055037
These results revealed, for the first time, the extracellular role of syntaxin4 and shed light on the division of the extracellular effects exerted by epimorphin and syntaxin4 on keratinocyte cornification. PMID: 22226963
Syntaxin 4 activation and insulin release in the absence of the glucose stimulus, consistent with nitrosative stress and dysfunctional exocytosis PMID: 21393240
Stx4 is an essential postsynaptic component for synaptic plasticity in hippocampal neurons. PMID: 20959521
Syntaxin 4 is required for acid sphingomyelinase activity and apoptotic function. PMID: 20956541
dysferlin and syntaxin-4 similarly transit a common endosomal pathway in skeletal muscle cells. PMID: 20595382
Stx4 defines an exocytic zone that directs membrane fusion for postsynaptic plasticity, revealing a novel specialization for local membrane traffic in dendritic spines. PMID: 20434989
Syntaxin 4 and Synip (syntaxin 4 interacting protein) regulate insulin secretion in the pancreatic beta HC-9 cells PMID: 12855681
syntaxin 4 is involved in the intracellular transport of MT1-MMP toward the plasma membrane PMID: 15351710

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Subcellular Location

Cell membrane; Single-pass type IV membrane protein. Cell projection, neuron projection.

Protein Families

Syntaxin family

Tissue Specificity

Expressed in neutrophils and neutrophil-differentiated HL-60 cells. Expression in neutrophils increases with differentiation.

Database Links

HGNC: 11439

OMIM: 186591

KEGG: hsa:6810

STRING: 9606.ENSP00000317714

UniGene: Hs.83734

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Code	CSB-RA290392A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: 293 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, HL-60 whole cell lysate, A375 whole cell lysate, MCF-7 whole cell lysate All lanes: Syntaxin 4 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35, 34 kDa Observed band size: 35 kDa
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STX4 Recombinant Monoclonal Antibody

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