SUMO2/SUMO3 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA975045A0HU
Size	US$210
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Image	Immunofluorescence staining of HeLa cell with CSB-RA975045A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA975045A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Details
Customer Reviews and Q&A

Product Details

Uniprot No.

P61956 P55854

Target Names

SUMO2/SUMO3

Alternative Names

HSMT 3 antibody; HSMT3 antibody; MGC117191 antibody; Sentrin 2 antibody; Sentrin-2 antibody; Sentrin2 antibody; Small ubiquitin like modifier 2 antibody; Small ubiquitin related modifier 2 antibody; Small ubiquitin-related modifier 2 antibody; SMT 3B antibody; SMT3 homolog 2 antibody; SMT3 suppressor of mif two 3 homolog 2 antibody; SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae) antibody; SMT3, yeast, homolog 2 antibody; Smt3A antibody; SMT3B antibody; SMT3H2 antibody; SUMO-2 antibody; SUMO-3 antibody; SUMO2 antibody; SUMO2_HUMAN antibody; Sumo3 antibody; Ubiquitin like protein SMT3B antibody; Ubiquitin-like protein SMT3A antibody; Small ubiquitin like modifier 3 antibody; Small ubiquitin related modifier 3 antibody; Small ubiquitin-related modifier 3 antibody; SMT3 homolog 1 antibody; SMT3 suppressor of mif two 3 homolog 1 antibody; SMT3 suppressor of mif two 3 homolog 3 antibody; SMT3, yeast, homolog 1 antibody; SMT3A antibody; Smt3B antibody; SMT3H1 antibody; SUMO-2 antibody; SUMO-3 antibody; sumo3 antibody; SUMO3_HUMAN antibody; Ubiquitin like protein SMT3A antibody; Ubiquitin-like protein SMT3B antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human SUMO2/SUMO3 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

14A3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IF, FC

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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