SYNCRIP Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA984434A0HU
Size	US$210
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Image	IHC image of CSB-RA984434A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA984434A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela Cells with CSB-RA984434A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Product Details
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Target Background

Product Details

Uniprot No.

O60506

Target Names

SYNCRIP

Alternative Names

Heterogeneous nuclear ribonucleoprotein Q (hnRNP Q) (Glycine- and tyrosine-rich RNA-binding protein) (GRY-RBP) (NS1-associated protein 1) (Synaptotagmin-binding, cytoplasmic RNA-interacting protein), SYNCRIP, HNRPQ NSAP1

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human hnRNP Q

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9C7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:20-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SYNCRIP, also known as hnRNP Q or NSAP1)0, is an evolutionarily conserved RBP across eukaryotic organisms and participates in regulating various aspects of neuronal development and plasticity. It plays important role in neuronal, myeloid leukemia stem cell, and muscular development. Abnormal expression of SYNCRIP is associated with immune response disorders and neurodegenerative disorders. On the RNA side, SYNCRIP has multiple roles in the control of RNA metabolism through recognizing a variety of sequences and regulating pre-mRNA splicing, translation, transport as well as degradation.

The main steps in the production of this SYNCRIP recombinant antibody include immunization; harvest of positive spleen cells; obtaining the antibody sequence by screening and sequencing; expression of the target antibody in mammalian cells; purification. The SYNCRIP antibody was produced recombinantly and has many advantages: high reproducibility, specificity and scalability. And has been validated in ELISA, IHC, IF.

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Target Background

Function

Heterogenous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD-mediated complex that promotes MYC mRNA stability. Isoform 1, isoform 2 and isoform 3 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences. Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 3 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation; seems not to be essential for GAIT complex function.

Gene References into Functions

A cryptic RNA-binding domain mediates Syncrip recognition and exosomal partitioning of miRNA targets. PMID: 29483512
our data indicate that hnRNP Q1 is a novel trans-acting factor that binds to Aurora-A mRNA 5'-UTRs and regulates its translation, which increases cell proliferation and contributes to tumorigenesis in colorectal cancer PMID: 28079881
SYNCRIP is required for survival of leukemia cells. SYNCRIP controls the myeloid leukemia stem cell program. PMID: 28436985
The acidic domain is a unique structural feature of the splicing factor SYNCRIP. PMID: 27081926
hnRNPQ6 is required for APOBEC1-enhanced IL8 production. PMID: 25100733
The hnRNP Q is a novel substrate of SHP2 and the SHP2 activity may be also involved in RNA metabolisms via dephosphorylation of hnRNP Q. PMID: 23946508
The inhibitory effect of hnRNP Q on YB-1 mRNA translation can be explained by its ability to pro-mote YB-1 binding to the regulatory element within the YB-1 mRNA 3' UTR. PMID: 23980891
These data indicate that hnRNP Q can stimulate the protein production of HIV-1 Rev-dependent mRNAs without changing mRNA levels and mRNA export, respectively. PMID: 23679954
Data demonstrate that expression levels of hnRNP A1, Q, K, R, and U influence HIV-1 production by persistently infected PMID: 19808671
The overexpression of NSAP1 specifically enhanced HCV IRES-dependent translation, and knockdown of NSAP1 by use of a small interfering RNA specifically inhibited the translation of HCV mRNA. PMID: 15340051
SYNCRIP is transported within the dendrite as a component of mRNA granules PMID: 15475564
methylation of hnRNPQ is important for its nuclear localization PMID: 16765914
Results suggest hnRNPQ interacts via its acidic domain (AcD) with Apobec1 and that this interaction is regulated by AcD phosphorylation. PMID: 17010310
Data demonstrate that hnRNP Q is a splicing modulator of SMN2, further underscoring the potential of hnRNP Q as a therapeutic target for spinal muscular atrophy. PMID: 18794368
Results demonstrate that galectin-3 stabilizes hnRNP Q via complex formation, and reduction in the hnRNP Q level leads to slow proliferation and less susceptibility to 5-FU. PMID: 19137262
SYNCRIP participates in both RNA replication and translation in HCV life cycle. PMID: 19232660

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Subcellular Location

Cytoplasm. Microsome. Endoplasmic reticulum. Nucleus.; [Isoform 1]: Nucleus, nucleoplasm.; [Isoform 2]: Nucleus, nucleoplasm.; [Isoform 3]: Nucleus, nucleoplasm.

Tissue Specificity

Ubiquitously expressed. Detected in heart, brain, pancreas, placenta, spleen, lung, liver, skeletal muscle, kidney, thymus, prostate, uterus, small intestine, colon, peripheral blood and testis.

Database Links

HGNC: 16918

KEGG: hsa:10492

STRING: 9606.ENSP00000358635

UniGene: Hs.571177

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