TCP1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA056950A0HU
Size	US$210
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Image	Immunofluorescence staining of Hela cell with CSB-RA056950A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing jurkat cells stained with CSB-RA056950A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

P17987

Target Names

TCP1

Alternative Names

T-complex protein 1 subunit alpha (TCP-1-alpha) (CCT-alpha), TCP1, CCT1 CCTA

Species Reactivity

Human

Immunogen

A synthesized peptide from human TCP1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

20F10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IF, FC

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

TCP1, also known as T-complex protein 1 subunit alpha, serves as an essential component of the chaperonin-containing TCP1 complex (CCT), a molecular chaperone system critical for the proper folding of newly synthesized proteins. This complex plays a particularly important role in the folding of actin, tubulin, and other cytoskeletal proteins, making TCP1 a significant target for researchers investigating protein quality control, cytoskeletal dynamics, and cellular stress responses.

This recombinant monoclonal antibody, clone 20F10, offers the reproducibility and consistency that demanding experimental workflows require. Generated against a synthetic peptide derived from human TCP1 and produced using recombinant technology, this antibody provides sequence-defined specificity with minimal lot-to-lot variation, ensuring that your results remain comparable across extended studies and collaborative projects.

Validation studies demonstrate reliable performance in immunofluorescence and flow cytometry applications. Immunofluorescence staining of HeLa cells reveals clear cytoplasmic localization consistent with TCP1's known distribution pattern, with optimal results achieved at dilutions between 1:50 and 1:200. Flow cytometric analysis using Jurkat cells shows distinct positive population shifts compared to isotype controls, confirming the antibody's utility for quantitative single-cell analysis of TCP1 expression levels.

The unconjugated format provides flexibility for pairing with your preferred secondary detection systems, while the liquid formulation in glycerol-containing buffer ensures stability during long-term storage. Whether you are exploring chaperone biology, investigating proteostasis networks, or examining cytoskeletal protein maturation pathways, this antibody delivers the specificity and consistency needed for meaningful experimental outcomes.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. The TRiC complex plays a role in the folding of actin and tubulin.

Gene References into Functions

Chaperonin CCT interferes with fibre assembly by interaction of its CCTzeta and CCTgamma subunits with the A53T central hydrophobic region. This interaction is specific to NAC conformation, as it is produced once soluble alpha-synuclein A53T oligomers form and blocks the reaction before fibres begin to grow. PMID: 28102321
chaperonin-containing TCP-1 complex required for lysosomal functioning and autophagosome degradation PMID: 27929117
Result suggest the positive correlation between purinergic receptor P2X 7 (P2X7R) and T-complex protein 1 (TCP-1) in lymphoma patients. PMID: 26556873
Data suggest that biosynthesis and folding of leukemogenic fusion oncoprotein AML1-ETO/RUNX1-RUNX1T1 is facilitated by interaction with the chaperonin TRiC/CCT1/TCP1 and HSP70 (heat shock protein 70). PMID: 26706127
Changes for CRMP2, TCP1epsilon, TPM2 and 14-3-3gamma were confirmed in experimental tumors and in a series of 28 human SI-NETs. PMID: 25224486
A role for the TRiC subunits TCP1 and CCT2, and potentially the entire TRiC complex, in breast cancer. PMID: 25704758
CCT8 might be an oncogene and participate in HCC cell proliferation. PMID: 24862099
identified 6 of the 8 components of the chaperonin-containing TCP-1 (CCT) complex bound to LOX-1 cytoplasmic domain PMID: 24846140
Data suggest that specific molecular mediators involved in glucocerebrosidase maturation and degradation, and abnormal interaction with TCP1 and c-Cbl, could be responsible for phenotypic variation among patients with the same genotypes. PMID: 21098288
the membrane binding domain of CTP:phosphocholine cytidylyltransferase has a role in alveolar type II cells PMID: 19684306
Expression patterns of chaperone proteins in cerebral cortex of the fetus with Down syndrome: dysregulation of T-complex protein 1. PMID: 11771755
TRiC chaperonin binds to HIF prolyl hydroxylase PHD3 PMID: 15251459
the strong inhibitory action of PhLP(S) on Gbetagamma signaling is the result of a previously unrecognized mechanism of Gbetagamma-regulation, inhibition of Gbetagamma-folding by interference with TCP-1alpha PMID: 15745879
Data show that Plk1 associates with chaperonin-containing TCP1 complex (CCT) both in vitro and in vivo. PMID: 15923617
The specific and directed rearrangement of the beta-actin structure, seen in the natural beta-actin-TRiC system, is vital for guiding beta-actin to the native state. PMID: 17939680
a paper describing a pathway in which this gene product functions PMID: 1630491

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Subcellular Location

Cytoplasm, cytosol. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

Protein Families

TCP-1 chaperonin family

Database Links

HGNC: 11655

OMIM: 186980

KEGG: hsa:6950

STRING: 9606.ENSP00000317334

UniGene: Hs.363137

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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