Product Details

Uniprot No.

Target Names

TP53INP1

Alternative Names

DKFZp434M1317 antibody; FLJ22139 antibody; p53 damage inducible nuclear protein 1 antibody; p53 dependent damage inducible nuclear protein 1 antibody; p53 inducible nuclear protein 1 antibody; p53 inducible p53DINP1 antibody; p53-dependent damage-inducible nuclear protein 1 antibody; p53DINP1 antibody; SIP antibody; Stress induced protein antibody; Stress-induced protein antibody; T53I1_HUMAN antibody; Teap antibody; TP53 DINP1 antibody; TP53 INP1 antibody; TP53DINP1 antibody; TP53INP1 antibody; TP53INP1A antibody; TP53INP1B antibody; Tumor protein p53 inducible nuclear protein 1 antibody; Tumor protein p53-inducible nuclear protein 1 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human TP53INP1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

20H7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, FC

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Protocols

ELISA Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

TP53INP1, also known as tumor protein p53-inducible nuclear protein 1 or stress-induced protein, serves as a critical mediator of p53-dependent cellular stress responses. This nuclear protein is upregulated following genotoxic stress and oxidative damage, where it functions as a key regulator of cell cycle arrest and apoptosis. Its involvement in p53-mediated tumor suppression pathways makes TP53INP1 a significant target for researchers investigating DNA damage responses, cellular stress mechanisms, and cancer biology.

This recombinant monoclonal antibody, clone 20H7, offers the reproducibility and consistency that demanding research applications require. Developed using recombinant technology with a sequence-defined approach, this rabbit IgG antibody eliminates the lot-to-lot variability that can complicate longitudinal studies and multi-site collaborations. The antibody was raised against a synthesized peptide derived from human TP53INP1 protein and subsequently purified through affinity chromatography to ensure high specificity.

Validation studies demonstrate reliable performance in flow cytometry applications using human samples. Testing in PC-3 cells, a well-established human prostate cancer cell line, showed clear positive signal separation from isotype control when cells were fixed, permeabilized, and stained at a 1:100 dilution. This validation confirms the antibody's utility for intracellular detection of TP53INP1 in fixed cell populations, with recommended working dilutions ranging from 1:50 to 1:200 for flow cytometry experiments.

For researchers exploring stress response pathways, p53 signaling networks, or tumor suppressor mechanisms, this antibody provides a dependable tool for characterizing TP53INP1 expression at the single-cell level through flow cytometric analysis.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Antiproliferative and proapoptotic protein involved in cell stress response which acts as a dual regulator of transcription and autophagy. Acts as a positive regulator of autophagy. In response to cellular stress or activation of autophagy, relocates to autophagosomes where it interacts with autophagosome-associated proteins GABARAP, GABARAPL1/L2, MAP1LC3A/B/C and regulates autophagy. Acts as an antioxidant and plays a major role in p53/TP53-driven oxidative stress response. Possesses both a p53/TP53-independent intracellular reactive oxygen species (ROS) regulatory function and a p53/TP53-dependent transcription regulatory function. Positively regulates p53/TP53 and p73/TP73 and stimulates their capacity to induce apoptosis and regulate cell cycle. In response to double-strand DNA breaks, promotes p53/TP53 phosphorylation on 'Ser-46' and subsequent apoptosis. Acts as a tumor suppressor by inducing cell death by an autophagy and caspase-dependent mechanism. Can reduce cell migration by regulating the expression of SPARC.

Gene References into Functions

Upregulated miR-200a enhances treatment resistance via antagonizing TP53INP1 and YAP1 in breast cancer. PMID: 29329575
these results revealed that TP53INP1 is a target gene of miR-504 and that miR-504 enhances osteosarcoma growth and promotes distant metastases by targeting TP53INP1. Thus, miR-504/TP53INP1 may be associated with osteosarcoma size and clinical stage. PMID: 29048685
Low TP53INP1 expression is associated with Metastasis of Hepatocellular Carcinoma. PMID: 28674078
Negative TP53INP1 protein levels correlated with a poor outcome in pediatric ependymoma. Direct binding of miR-124-3p to its target TP53INP1 is demonstrated. PMID: 28437838
Results showed that TP53INP1 and E-cadherin mRNA and protein were significantly down-regulated in glioma tumors and positively correlated with higher grade and poor survival. Also, the study provides evidence that TP53INP1 3'-UTR could inhibit the epithelial-mesenchymal transition, thus hindering the migration and invasion of glioma via acting as a competitive endogenous RNA for E-cadherin. PMID: 27341836
miR-205/TP53INP1 mediated autophagy pathway might be an important molecular mechanism regulating radiosensitivity of prostate cancer cells PMID: 26813458
silencing of TP53inp1 leads radiation induced autophagy impairment and induces accumulation of damaged mitochondria in primary human fibroblasts. PMID: 26512655
MicroRNA205 promotes the tumorigenesis of nasopharyngeal carcinoma through targeting TP53INP1 protein. PMID: 26252115
FOXP1, TP53INP1, TNFAIP3, and TUSC2 were identified as miR-19a targets. PMID: 26367773
GWAS identifies TP53INP1 as new susceptibility gene for Alzheimer's disease. PMID: 24922517
TP53INP1 SUMOylation is essential for the regulation of p53 activity induced by oxidative stress. PMID: 24608790
TGFB1 induced miR-155 regulates TP53INP1 expression, epithelial-mesenchymal transition and acquisition of a stem cell phenotype. PMID: 25633840
miR-155 may play an important role in promoting the generation of stem cell-like cells and their self-renewal by targeting the gene TP53INP1. PMID: 25601564
Results demonstrate that miR-569 is overexpressed in a subset of ovarian and breast cancers and alters cell survival and proliferation through downregulation of TP53INP1 expression. PMID: 25490449
Study reveals that miR-155 acts as an oncogene by targeting TP53INP1 in esophageal squamous cell carcinoma. PMID: 24551280
Expression of miR-155 is significantly higher in MCF-7 cells compared with MDA-MB-231 cells. Ectopic expression of TP53INP1 inhibits growth of MCF-7 cells by inducing cell apoptosis and inhibiting cell cycle progression. PMID: 24152184
miR-182/TP53INP1 signaling represents a novel pathway regulating chemoresistance in cisplatin-treated hepatocellular carcinoma PMID: 24447717
Upregulation of miR-155, mediated by estradiol, and consequent downregulation of TP53INP1 potentially promotes breast cancer development and progression. PMID: 23568502
Fndings indicate that miR-17-5p/20a promote gastric cancer cell proliferation and inhibit cell apoptosis via post-transcriptional modulation of p21 and TP53INP1. PMID: 23333058
miR-17-5p functions as a tumor suppressor in cervical cancer cells by targeting tumor protein p53-induced nuclear protein 1. PMID: 22730212
TP53INP1 antisense oligonucleotide inhibits proliferation and induces apoptosis in castration-sensitive LNCaP tumor cells. PMID: 22213058
TP53INP1 identified two conserved regions in the DOR family that concentrate multiple functions crucial for autophagy and transcription. PMID: 22470510
In normal prostate tissues, TP53INP1 is only expressed in prostate basal cells. There is a de novo TP53INP1 expression in prostate luminal cells in inflammatory prostate tissues, high grade PIN lesions and in prostate cancer. PMID: 21538421
This novel TP53INP1 activity on the regulation of SPARC expression could explain in part its tumor suppressor function in pancreatic adenocarcinoma by modulating cellular spreading during the metastatic process PMID: 21339733
TP53INP1 is a tumor suppressor in esophageal squamous cell carcinoma (ESCC0; c-Myc-mediated DNA methylation-associated silencing of TP53INP1 contributed to the pathogenesis of human ESCC. PMID: 21219856
Data show that induction of the stress-induced proteins (SIPs) SIP18 and SIP27, in human- and mouse-derived cell lines, is absent from cells with deleted, mutated or inactive p53, suggesting that regulation of SIP gene expression is dependent on p53. PMID: 12067065
Assignment of tumor protein p53 induced nuclear protein 1 (TP53INP1) gene to human chromosome band 8q22 by in situ hybridization. PMID: 12438758
TP53INP1s and HIPK2 could be partners in regulating p53 activity. PMID: 12851404
TP53INP1s are functionally associated with p73 to regulate cell cycle progression and apoptosis. PMID: 16044147
TP53INP1 protein negativity is significantly associated with aggressive pathological phenotypes of gastric cancer. PMID: 16521180
TP531NP1 plays a significant role in the progression of anaplastic carcinoma or contributes to anaplastic transformation from papillary or follicular carcinoma, which is in sharp contrast to findings in previous in vitro and in vivo studies. PMID: 17393983
PLZF upregulates apoptosis-inducer TP53INP1, ID1, and ID3 genes, and downregulates the apoptosis-inhibitor TERT gene PMID: 17537403
The p53DINP1 mRna expression in the antral mucosa was significantly lower in the gastric cancer patients than in the chronic gastritis patients. PMID: 18277906
P53DINP1 mRNA expression promotes Ser46 phophlrylation of p53, without finding a significant correlation between mRNA expression of p53AIP1. PMID: 18277909
findings implicate a miR-93/miR-130b-TP53INP1 axis that affects the proliferation and survival of HTLV-1-infected/transformed cells PMID: 18974142

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Subcellular Location

Cytoplasm, cytosol. Nucleus. Nucleus, PML body. Cytoplasmic vesicle, autophagosome. Note=Shuttles between the nucleus and the cytoplasm, depending on cellular stress conditions, and re-localizes to autophagosomes on autophagy activation.

Tissue Specificity

Ubiquitously expressed.

Database Links

HGNC: 18022

OMIM: 606185

KEGG: hsa:94241

STRING: 9606.ENSP00000344215

UniGene: Hs.492261

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Code	CSB-RA277581A0HU
Size	US$210
	Order now
Image	Overlay Peak curve showing PC-3 cells stained with CSB-RA277581A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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TP53INP1 Recombinant Monoclonal Antibody

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