VAMP8 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA843784A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: HL-60 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), SW620 whole cell lysate(30µg), K562 whole cell lysate(30µg), HeLa whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg), Mouse liver tissue lysate(30µg), Mouse kidney tissue lysate(30µg) All lanes: VAMP8 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 11 kDa Observed band size: 15 kDa Exposure time: 2min Overlay Peak curve showing Hela cells stained with CSB-RA843784A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q9BV40

Target Names

VAMP8

Alternative Names

Vesicle-associated membrane protein 8 (VAMP-8) (Endobrevin) (EDB), VAMP8

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human VAMP8

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

2B8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

VAMP8, also known as endobrevin, is a SNARE protein that plays essential roles in membrane fusion events critical to vesicular trafficking, autophagy, and regulated secretion. As a key mediator of endosomal and lysosomal fusion processes, VAMP8 has become an important target for researchers investigating autophagy pathways, platelet degranulation, mast cell exocytosis, and intracellular trafficking mechanisms.

This recombinant monoclonal antibody, generated from clone 2B8, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and produced recombinantly in rabbit host cells, researchers benefit from lot-to-lot uniformity that eliminates the variability often encountered with traditional hybridoma-derived antibodies. Affinity chromatography purification ensures high specificity for your VAMP8 detection needs.

Validation studies demonstrate reliable performance across multiple applications and sample types. In western blot experiments, this antibody detects VAMP8 in a diverse panel of human cell lines including HL-60, MCF-7, SW620, K562, and HeLa cells, with cross-species reactivity confirmed in mouse NIH/3T3 cells as well as mouse liver and kidney tissue lysates. The observed band at approximately 15 kDa runs slightly higher than the predicted 11 kDa molecular weight, a shift commonly attributed to post-translational modifications such as glycosylation. Flow cytometry validation in HeLa cells confirms utility for intracellular protein detection in fixed and permeabilized samples, providing flexibility for researchers studying VAMP8 expression at the single-cell level.

Whether investigating autophagosome-lysosome fusion, secretory pathway dynamics, or immune cell degranulation, this antibody provides a dependable tool for advancing your understanding of VAMP8 biology.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex. Also required for dense-granule secretion in platelets. Plays also a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes. Participates also in the activation of type I interferon antiviral response through a TRIM6-dependent mechanism.

Gene References into Functions

our study suggested that VAMP8 gene variants might not contribute to glioma susceptibility and associated with glioma in the Chinese Han population. PMID: 26191281
Vesicle-associated membrane protein 8 as a novel oncogene by promoting cell proliferation and therapeutic resistance in glioma. Targeting VAMP8 may serve as a potential therapeutic regimen for the treatment of glioma. PMID: 25209430
Starvation-induced MTMR13 and RAB21 activity regulates VAMP8 to promote autophagosome-lysosome fusion. PMID: 25648148
Cytotoxic granule exocytosis is a sequential, multivesicle fusion process requiring VAMP8-mediated recycling endosome fusion before cytotoxic granule fusion. PMID: 26124288
Inhibition of VAMP8, but not VAMP7, significantly reduces viral entry. PMID: 24550300
VAMP8 regulates mucin granule exocytosis in airway goblet cells, and reduction of its expression may provide a novel therapeutic target to ameliorate airway mucus obstruction in lung diseases PMID: 22144578
Assessment of KIF6 genotype is not useful in predicting low density lipoprotein cholesterol lowering response to pravastatin, and heart disease risk reduction in the elderly. PMID: 22192511
The VAMP8 rs1010 polymorphism was associated with CHD risk in Chinese Han population, the A allele might serve as a genetic risk factor of coronary heart disease. PMID: 20533276
Results suggest that the combinational SNARE proteins VAMP8 and Vti1b mediate the fusion of antimicrobial and canonical autophagosomes with lysosomes, an essential event for autophagic degradation. PMID: 20089838
Support a role for VAMP8/endobrevin in the heterogeneity of platelet reactivity, and suggest a role for microRNA-96 in the regulation of VAMP8 expression. PMID: 19943878
VAMP8 is not essential for constitutive exocytosis, although it can be involved in the exocytosis PMID: 19738360
VAMP-3 and VAMP-8 are present in human platelets and are required for granule secretion. PMID: 12130530
Our findings indicate that VAMP5 and VAMP8 are not involved in POAG in the Dutch population. PMID: 16110299
VAMP-8 is unlikely to be a v-SNARE for constitutive exocytosis in HSY cells. PMID: 16195891
Variants in 2 genes were associated with early-onset myocardial infarct: VAMP8, which is involved in platelet degranulation, and HNRPUL1, which encodes a ribonuclear protein. PMID: 16690874
Data show that mature human mast cells express a specific pattern of SNARE and that VAMP-7 and VAMP-8, but not VAMP-2, are required for rapid degranulation. PMID: 18253931
A putative association between a VAMP8 gene variant and platelet functions ex vivo, was tested. PMID: 19374688

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Subcellular Location

Lysosome membrane; Single-pass type IV membrane protein. Early endosome membrane; Single-pass type IV membrane protein. Late endosome membrane; Single-pass type IV membrane protein. Cell membrane; Single-pass type IV membrane protein. Zymogen granule membrane; Single-pass type IV membrane protein.

Protein Families

Synaptobrevin family

Tissue Specificity

Platelets.

Database Links

HGNC: 12647

OMIM: 603177

KEGG: hsa:8673

STRING: 9606.ENSP00000263864

UniGene: Hs.714302

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Autophagy

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No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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