Kanamycin Monoclonal Antibody

Code CSB-MA000511I0m
Size US$350 How to order?
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Product Details

Description

The mice spleens isolated from kanamycin-BSA-immunized mice are fused with myeloma cells to generate hybridomas. Hybridoma cells are further injected into the abdominal cavity of mice, producing and obtaining mouse ascites fluid containing kanamycin monoclonal antibody. This monoclonal antibody occurs as an unconjugated IgG1. Its purity is greater than 95% using protein G purified. The target kanamycin is an aminoglycoside antibiotic used to treat severe bacterial infections and tuberculosis. And this anti-kanamycin monoclonal antibody has been validated for use in the ELISA assay.

Target Names KA
Alternative Names Amkin, biograncetin, enterokan
Raised in mouse
Immunogen Kanamycin-BSA conjugate
Conjugate Non-conjugated
Clonality monoclonal
Isotype IgG1
Purification Method >95%,protein G purifed
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form liquid
Tested Applications ELISA
Protocols ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Applications : Determination of Antibody Affinity

Review: For testing the affinity of corresponding mAb towards kanamycin-BSA conjugate in an ELISA, determination of antibody affinity was performed. It suggested that high affinity of respective antibody against their antigen-conjugate could be detected with EC50-values of 177.7 ng·mL-1 for kanamycin-specific mAb. Absorbance was measured after 10 min of ABTS substrate incubation. n = 1.

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