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| Code | CSB-MA000511I0m |
| Size | US$350 |
| Have Questions? | Leave a Message or Start an on-line Chat |
| Description |
The mice spleens isolated from kanamycin-BSA-immunized mice are fused with myeloma cells to generate hybridomas. Hybridoma cells are further injected into the abdominal cavity of mice, producing and obtaining mouse ascites fluid containing kanamycin monoclonal antibody. This monoclonal antibody occurs as an unconjugated IgG1. Its purity is greater than 95% using protein G purified. The target kanamycin is an aminoglycoside antibiotic used to treat severe bacterial infections and tuberculosis. And this anti-kanamycin monoclonal antibody has been validated for use in the ELISA assay. |
| Target Names | KA |
| Alternative Names | Amkin, biograncetin, enterokan |
| Raised in | mouse |
| Immunogen | Kanamycin-BSA conjugate |
| Conjugate | Non-conjugated |
| Clonality | monoclonal |
| Isotype | IgG1 |
| Purification Method | >95%，protein G purifed |
| Concentration | It differs from different batches. Please contact us to confirm it. |
| Buffer | Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Form | liquid |
| Tested Applications | ELISA |
| Protocols | ELISA Protocol |
| Troubleshooting and FAQs | Antibody FAQs |
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Lead Time | Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
Applications : Determination of Antibody Affinity
Review: For testing the affinity of corresponding mAb towards kanamycin-BSA conjugate in an ELISA, determination of antibody affinity was performed. It suggested that high affinity of respective antibody against their antigen-conjugate could be detected with EC50-values of 177.7 ng·mL-1 for kanamycin-specific mAb. Absorbance was measured after 10 min of ABTS substrate incubation. n = 1.
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