A Novel Method for Antibiotic Detection in Milk Based on Competitive Magnetic Immunodetection. Jan Pietschmann,Foods (Basel, Switzerland),2020
Applications: Determination of Antibody Affinity
Review: For testing the affinity of corresponding mAb towards kanamycin-BSA conjugate in an ELISA, determination of antibody affinity was performed. It suggested that high affinity of respective antibody against their antigen-conjugate could be detected with EC50-values of 177.7 ng·mL-1 for kanamycin-specific mAb. Absorbance was measured after 10 min of ABTS substrate incubation. n = 1.